The targeted delivery of multicomponent cargos to cancer cells by nanoporous particle-supported lipid bilayers.

Encapsulation of drugs within nanocarriers that selectively target malignant cells promises to mitigate side effects of conventional chemotherapy and to enable delivery of the unique drug combinations needed for personalized medicine. To realize this potential, however, targeted nanocarriers must simultaneously overcome multiple challenges, including specificity, stability and a high capacity for disparate cargos. Here we report porous nanoparticle-supported lipid bilayers (protocells) that synergistically combine properties of liposomes and nanoporous particles. Protocells modified with a targeting peptide that binds to human hepatocellular carcinoma exhibit a 10,000-fold greater affinity for human hepatocellular carcinoma than for hepatocytes, endothelial cells or immune cells. Furthermore, protocells can be loaded with combinations of therapeutic (drugs, small interfering RNA and toxins) and diagnostic (quantum dots) agents and modified to promote endosomal escape and nuclear accumulation of selected cargos. The enormous capacity of the high-surface-area nanoporous core combined with the enhanced targeting efficacy enabled by the fluid supported lipid bilayer enable a single protocell loaded with a drug cocktail to kill a drug-resistant human hepatocellular carcinoma cell, representing a 10(6)-fold improvement over comparable liposomes

Versatile transporter apparatus for experiments with optically trapped Bose-Einstein condensates

We describe a versatile and simple scheme for producing magnetically and optically-trapped Rb-87 Bose-Einstein condensates, based on a moving-coil transporter apparatus. The apparatus features a TOP trap that incorporates the movable quadrupole coils used for magneto-optical trapping and long-distance magnetic transport of atomic clouds. As a stand-alone device, this trap allows for the stable production of condensates containing up to one million atoms. In combination with an optical dipole trap, the TOP trap acts as a funnel for efficient loading, after which the quadrupole coils can be retracted, thereby maximizing optical access. The robustness of this scheme is illustrated by realizing the superfluid-to-Mott insulator transition in a three-dimensional optical lattice

Creation of gap solitons in Bose-Einstein condensates

We discuss a method to launch gap soliton-like structures in atomic Bose-Einstein condensates confined in optical traps. Bright vector solitons consisting of a superposition of two hyperfine Zeeman sublevels can be created for both attractive and repulsive interactions between the atoms. Their formation relies on the dynamics of the atomic internal ground states in two far-off resonant counterpropagating sigma^+ sigma^- polarized laser beams which form the optical trap. Numerical simulations show that these solitons can be prepared from a one-component state provided with an initial velocity.Comment: 6 pages, 3 figure

Intelligent Software for Ecological Building Design

Building design is a complex process because of the number of elements and issues involved and the number of relationships that exist among them. Adding sustainability issues to the list increases the complexity of design by an order of magnitude. There is a need for computer assistance to manage the increased complexity of design and to provide intelligent collaboration in formulating acceptable design solutions. Software development technology today offers opportunities to design and build an intelligent software system environment that can serve as a reliable intelligent partner to the human designer. In this paper the authors discuss the requirements for an intelligent software design environment, explain the major challenges in designing this environment, propose an architecture for an intelligent design support system for sustainable design and present the existing technologies that can be used to implement that architecture

Physics with Coherent Matter Waves

This review discusses progress in the new field of coherent matter waves, in particular with respect to Bose-Einstein condensates. We give a short introduction to Bose-Einstein condensation and the theoretical description of the condensate wavefunction. We concentrate on the coherence properties of this new type of matter wave as a basis for fundamental physics and applications. The main part of this review treats various measurements and concepts in the physics with coherent matter waves. In particular we present phase manipulation methods, atom lasers, nonlinear atom optics, optical elements, interferometry and physics in optical lattices. We give an overview of the state of the art in the respective fields and discuss achievements and challenges for the future

Atom Interferometers

Interference with atomic and molecular matter waves is a rich branch of atomic physics and quantum optics. It started with atom diffraction from crystal surfaces and the separated oscillatory fields technique used in atomic clocks. Atom interferometry is now reaching maturity as a powerful art with many applications in modern science. In this review we first describe the basic tools for coherent atom optics including diffraction by nanostructures and laser light, three-grating interferometers, and double wells on AtomChips. Then we review scientific advances in a broad range of fields that have resulted from the application of atom interferometers. These are grouped in three categories: (1) fundamental quantum science, (2) precision metrology and (3) atomic and molecular physics. Although some experiments with Bose Einstein condensates are included, the focus of the review is on linear matter wave optics, i.e. phenomena where each single atom interferes with itself.Comment: submitted to Reviews of Modern Physic

Immunodetection of retinoblastoma-related protein and its phosphorylated form in interphase and mitotic alfalfa cells

Plant retinoblastoma-related (RBR) proteins are primarily considered as key regulators of G1/S phase transition, with functional roles in a variety of cellular events during plant growth and organ development. Polyclonal antibody against the C-terminal region of the Arabidopsis RBR1 protein also specifically recognizes the alfalfa 115 kDa MsRBR protein, as shown by the antigen competition assay. The MsRBR protein was detected in all cell cycle phases, with a moderate increase in samples representing G2/M cells. Antibody against the human phospho-pRb peptide (Ser807/811) cross-reacted with the same 115 kDa MsRBR protein and with the in vitro phosphorylated MsRBR protein C-terminal fragment. Phospho-MsRBR protein was low in G1 cells. Its amount increased upon entry into the S phase and remained high during the G2/M phases. Roscovitine treatment abolished the activity of alfalfa MsCDKA1;1 and MsCDKB2;1, and the phospho-MsRBR protein level was significantly decreased in the treated cells. Colchicine block increased the detected levels of both forms of MsRBR protein. Reduced levels of the MsRBR protein in cells at stationary phase or grown in hormone-free medium can be a sign of the division-dependent presence of plant RBR proteins. Immunolocalization of the phospho-MsRBR protein indicated spots of variable number and size in the labelled interphase nuclei and high signal intensity of nuclear granules in prophase. Structures similar to phospho-MsRBR proteins cannot be recognized in later mitotic phases. Based on the presented western blot and immunolocalization data, the possible involvement of RBR proteins in G2/M phase regulation in plant cells is discussed