Increased optical pathlength through aqueous media for the infrared microanalysis of live cells

The study of live cells using Fourier transform infrared spectroscopy (FTIR) and FTIR microspectroscopy (FT-IRMS) intrinsically yields more information about cell metabolism than comparable experiments using dried or chemically fixed samples. There are, however, a number of barriers to obtaining high-quality vibrational spectra of live cells, including correction for the significant contributions of water bands to the spectra, and the physical stresses placed upon cells by compression in short pathlength sample holders. In this study, we present a water correction method that is able to result in good-quality cell spectra from water layers of 10 and 12 μm and demonstrate that sufficient biological detail is retained to separate spectra of live cells based upon their exposure to different novel anti-cancer agents. The IR brilliance of a synchrotron radiation (SR) source overcomes the problem of the strong water absorption and provides cell spectra with good signal-to-noise ratio for further analysis. Supervised multivariate analysis (MVA) and investigation of average spectra have shown significant separation between control cells and cells treated with the DNA cross-linker PL63 on the basis of phosphate and DNA-related signatures. Meanwhile, the same control cells can be significantly distinguished from cells treated with the protein kinase inhibitor YA1 based on changes in the amide II region. Each of these separations can be linked directly to the known biochemical mode of action of each agent. Keywords: Synchrotron radiation (SR), Fourier transform infrared spectroscopy (FTIR), Infrared microspectroscopy (IRMS), Cancer, Single cell, Drug-cell interaction

Open-flow mixing: Experimental evidence for strange eigenmodes

We investigate experimentally the mixing dynamics in a channel flow with a finite stirring region undergoing chaotic advection. We study the homogenization of dye in two variants of an eggbeater stirring protocol that differ in the extent of their mixing region. In the first case, the mixing region is separated from the side walls of the channel, while in the second it extends to the walls. For the first case, we observe the onset of a permanent concentration pattern that repeats over time with decaying intensity. A quantitative analysis of the concentration field of dye confirms the convergence to a self-similar pattern, akin to the strange eigenmodes previously observed in closed flows. We model this phenomenon using an idealized map, where an analysis of the mixing dynamics explains the convergence to an eigenmode. In contrast, for the second case the presence of no-slip walls and separation points on the frontier of the mixing region leads to non-self-similar mixing dynamics.Comment: 12 pages, 8 figures

Consistent thermodynamics for spin echoes

Spin-echo experiments are often said to constitute an instant of anti-thermodynamic behavior in a concrete physical system that violates the second law of thermodynamics. We argue that a proper thermodynamic treatment of the effect should take into account the correlations between the spin and translational degrees of freedom of the molecules. To this end, we construct an entropy functional using Boltzmann macrostates that incorporates both spin and translational degrees of freedom. With this definition there is nothing special in the thermodynamics of spin echoes: dephasing corresponds to Hamiltonian evolution and leaves the entropy unchanged; dissipation increases the entropy. In particular, there is no phase of entropy decrease in the echo. We also discuss the definition of macrostates from the underlying quantum theory and we show that the decay of net magnetization provides a faithful measure of entropy change.Comment: 15 pages, 2 figs. Changed figures, version to appear in PR

Boltzmann-Shannon Entropy: Generalization and Application

The paper deals with the generalization of both Boltzmann entropy and distribution in the light of most-probable interpretation of statistical equilibrium. The statistical analysis of the generalized entropy and distribution leads to some new interesting results of significant physical importance.Comment: 5 pages, Accepted in Mod.Phys.Lett.

Teleworking practice in small and medium-sized firms: Management style and worker autonomy

In an empirical study of teleworking practices amongst small and medium-sized enterprises (SMEs) in West London, organisational factors such as management attitudes, worker autonomy and employment flexibility were found to be more critical than technological provision in facilitating successful implementation. Consequently, we argue that telework in most SMEs appears as a marginal activity performed mainly by managers and specialist mobile workers

Deconvolving Smooth Residence Time Distributions from Raw Solute Transport Data

A residence time distribution (RTD) provides a complete model of longitudinal mixing effects that can be robustly derived from experimental solute transport data. Maximum entropy deconvolution has been shown to recover RTDs from preprocessed laboratory data. However, data preprocessing is time consuming and may introduce errors. Assuming data were recorded using sensors with a linear response, it should be possible to deconvolve raw data without preprocessing. This paper uses synthetically generated raw data to demonstrate that the quality of the deconvolved RTD remains satisfactory when preprocessing steps involving data cropping or calibration are skipped. Provided noise levels are relatively low, filtering steps may also be omitted. However, a rough subtraction of background concentration is recommended as a minimal preprocessing step. Deconvolved RTDs often include small-scale fluctuations that are inconsistent with a well-mixed fully turbulent system. These are believed to be associated with oversampling and/or unsuitable interpolation functions used in the maximum entropy deconvolution process. This paper describes a new interpolation function—linear interpolation with an automatic moving average (LAMA)—and demonstrates that, in combination with fewer sample points (e.g., 20), it enables smoother RTDs to be generated. The two improvements, to deconvolve raw data and to generate smoother RTDs, have been validated with experimental data. Raw solute transport traces collected from a river were deconvolved after background subtraction. The deconvolved RTDs compare favorably with those generated from the more traditional advection-dispersion equation (ADE) and aggregated dead zone (ADZ) models, but provide more detail of mixing processes. A laboratory manhole solute transport data set was deconvolved with and without preprocessing using 40 sample points and linear interpolation. The raw data were also deconvolved using 20 sample points and LAMA interpolation. The two sets of RTDs deconvolved from the raw data show the same mixing trends as those deconvolved from preprocessed data. However, those deconvolved with LAMA interpolation and 20 sample points are significantly smoother

Application of Information Theory in Nuclear Liquid Gas Phase Transition

Information entropy and Zipf's law in the field of information theory have been used for studying the disassembly of nuclei in the framework of the isospin dependent lattice gas model and molecular dynamical model. We found that the information entropy in the event space is maximum at the phase transition point and the mass of the cluster show exactly inversely to its rank, i.e. Zipf's law appears. Both novel criteria are useful in searching the nuclear liquid gas phase transition experimentally and theoretically.Comment: 5 pages, 5 figure

Mobility Edge in Aperiodic Kronig-Penney Potentials with Correlated Disorder: Perturbative Approach

It is shown that a non-periodic Kronig-Penney model exhibits mobility edges if the positions of the scatterers are correlated at long distances. An analytical expression for the energy-dependent localization length is derived for weak disorder in terms of the real-space correlators defining the structural disorder in these systems. We also present an algorithm to construct a non-periodic but correlated sequence exhibiting desired mobility edges. This result could be used to construct window filters in electronic, acoustic, or photonic non-periodic structures.Comment: RevTex, 4 pages including 2 Postscript figure

Thermodynamic optimization of steady-flow industrial chemical processes

© 2018, The Author(s). Industrial steady-flow chemical processes are generally organised as a sequence of individually optimised operations. However, this may not achieve overall optimization since material (as recycle), heat and work transfers overall may not be well balanced. We introduce the idea of a preliminary overall thermodynamic balance to produce a reversible process, with the objective of minimising, for both economic and environmental reasons, the quality and quantity of energy used. This balance may later require adjustment to account for the realities of available materials and equipment. For this purpose, we introduce (i) a Carnot temperature, TCarnot, by which a Carnot machine (an engine which can operate as either a heat pump or a turbine) can supply the required heat at the correct temperature for a process to operate reversibly, that is with least energy, and (ii) the GH Diagram on which Carnot temperature-based processes are plotted in ?G–?H space. We demonstrate the utility of this analysis by simple application to the Haber–Bosch process for ammonia synthesis and by a sequence of operations for the synthesis of methanol. We also briefly introduce the state function exergy, which uses the natural environment as the reference base for energy in place of pure elements under standard conditions

Isospin influences on particle emission and critical phenomenon in nuclear dissociation

Features of particle emission and critical point behavior are investigated as functions of the isospin of disassembling sources and temperature at a moderate freeze-out density for medium-size Xe isotopes in the framework of isospin dependent lattice gas model. Multiplicities of emitted light particles, isotopic and isobaric ratios of light particles show the strong dependence on the isospin of the dissociation source, but double ratios of light isotope pairs and the critical temperature determined by the extreme values of some critical observables are insensitive to the isospin of the systems. Values of the power law parameter of cluster mass distribution, mean multiplicity of intermediate mass fragments ($IMF$), information entropy ($H$) and Campi's second moment ($S_2$) also show a minor dependence on the isospin of Xe isotopes at the critical point. In addition, the slopes of the average multiplicites of the neutrons ($N_n$), protons ($N_p$), charged particles ($N_{CP}$), and IMFs ($N_{imf}$), slopes of the largest fragment mass number ($A_{max}$), and the excitation energy per nucleon of the disassembling source ($E^*/A$) to temperature are investigated as well as variances of the distributions of $N_n$, $N_p$, $N_{CP}$, $N_{IMF}$, $A_{max}$ and $E^*/A$. It is found that they can be taken as additional judgements to the critical phenomena.Comment: 9 Pages, 8 figure