Effect of material, geometry, surface treatment and environment on the shear strength of single lap joints

The single lap joint is the most studied type of adhesive joint in the literature. However, the joint strength prediction of such joints is still a controversial issue as it involves a lot of factors that are difficult to quantify such as the overlap length, the yielding of the adherend, the plasticity of the adhesive and the bondline thickness. The most complicated case is that where the adhesive is brittle and the overlap long. In any case, there is still a problem that is even more difficult to take into account which is the durability. There is a lack of experimental data and design criteria when the joint is subjected to high, low or variable temperature and/or humidity. The objective of this work is to carry out and quantify the various variables affecting the strength of single lap joints in long term, especially the effect of the surface preparation. The Taguchi method is used to decrease the number of experimental tests. The effect of material, geometry, surface treatment and environment is studied and it is shown that the main effect is that of the overlap length. In order to quantify the influence of the adhesive (toughness and thickness), the adherend (yield strength and thickness), the overlap, the test speed, the surface preparation and durability on the lap shear strength, the experimental design technique of Taguchi was used in the present study. An experimental matrix of eighteen tests was designed and each test was repeated three times. The influence of the eight previously-mentioned variables could be assessed using the statistical software Statview®. In this paper a simple predictive equation is proposed for the design of single lap joints

Development of A Novel Site-Specific Mutagenesis Assay Using MALDI-ToF MS (SSMA-MS).

We have developed and validated a novel site-specific mutagenesis assay, termed SSMA-MS, which incorporates MALDI-ToF mass spectrometry (MALDI-MS) analysis as a means of determining the mutations induced by a single DNA adduct. The assay involves ligating an adducted deoxyoligonucleotide into supF containing pSP189 plasmid. The plasmid is transfected into human Ad293 kidney cells allowing replication and therefore repair or a mutagenic event to occur. Escherichia coli indicator bacteria are transformed with recovered plasmid and plasmids containing the insert are identified colormetrically, as they behave as frameshift mutations. The plasmid is then amplified and digested using a restriction cocktail of Mbo11 and Mnl1 to yield 12 bp deoxyoligonucleotides, which are characterized by MALDI-MS. MALDI-MS takes advantage of the difference in molecular weight between bases to identify any induced mutations. This analysis method therefore provides qualitative and quantitative information regarding the type and frequency of mutations induced. This assay was developed and validated using an O6-methyl-2′-deoxyguanosine adduct, which induced the expected GC→AT substitutions, when replicated in human or bacterial cells. This approach can be applied to the study of any DNA adduct in any biologically relevant gene sequence (e.g. p53) in human cells and would be particularly amenable to high-throughput analysis

ESM_RW2 from Advancing national climate change risk assessment to deliver national adaptation plans

Supplementary Material: Worked example and information about UKCP0

Additional file 1 of Reducing gut microbiome-driven adipose tissue inflammation alleviates metabolic syndrome

Additional file 1: Supplementary Figure S1. Myeloid cells are the primary cell type affected by TXN in the adipose tissue. Supplementary Figure S2. TXN treatment alters fecal microbiota composition and some Oscillibacter features. Supplementary Figure S3.  TXN treatment increases mitochondrial gene expression. Figure S4. Identification of overlapping gene expression between adipose tissue from TXN-treated mice vs. O.valericigenes supernatant-treated macrophage cell lines. Figure S5. Gene expression from in vitro titration of O. valericigenes and L. gasseri cell free supernatant on a IMM cell line

Additional file 3 of Reducing gut microbiome-driven adipose tissue inflammation alleviates metabolic syndrome

Additional file 3. Processed data of the single cell RNA sequencing from adipose tissue (WAT_filtered_feature_bc_matrix.h5)

Additional file 2 of Reducing gut microbiome-driven adipose tissue inflammation alleviates metabolic syndrome

Additional file 2: Table S1. Number of parameters per data type in each category in the network. Table S2. Summary table of parameters, including statistical information, cell type information, microbiota dependence/independence for genes, abundance of microbes, and network information. Table S3. IMM/RAW cell with Oscillibacter supplementation (data from GSE203488, GSE203516). Table S4. In vivo validation of O. valericigenes gavage (data from GSE215226). Table S5. RT-PCR  data from in vitro cell free  Oscillibacter supernatant titration on IMM cells

What is new for an old molecule? systematic review and recommendations on the use of Resveratrol

What is new for an old molecule? systematic review and recommendations on the use of Resveratro