Androgen receptor signalling in Vascular Endothelial cells is dispensable for spermatogenesis and male fertility

<p>Abstract</p> <p>Background</p> <p>Androgen signalling is essential both for male development and function of the male reproductive system in adulthood. Within the adult testis, Germ cells (GC) do not express androgen receptor (AR) suggesting androgen-mediated promotion of spermatogenesis must act via AR-expressing somatic cell-types. Several recent studies have exploited the Cre/lox system of conditional gene-targeting to ablate AR function from key somatic cell-types in order to establish the cell-specific role of AR in promotion of male fertility. In this study, we have used a similar approach to specifically ablate AR-signalling from Vascular Endothelial (VE) cells, with a view to defining the significance of androgen signalling within this cell-type on spermatogenesis.</p> <p>Findings</p> <p>AR expression in VE cells of the testicular vasculature was confirmed using an antibody against AR. A Cre-inducible fluorescent reporter line was used to empirically establish the utility of a mouse line expressing Cre Recombinase driven by the Tie2-Promoter, for targeting VE cells. Immunofluorescent detection revealed expression of YFP (and therefore Cre Recombinase function) limited to VE cells and an interstitial population of cells, believed to be macrophages, that did not express AR. Mating of Tie2-Cre males to females carrying a floxed AR gene produced Vascular Endothelial Androgen Receptor Knockout (VEARKO) mice and littermate controls. Ablation of AR from all VE cells was confirmed; however, no significant differences in bodyweight or reproductive tissue weights could be detected in VEARKO animals and spermatogenesis and fertility was unaffected.</p> <p>Conclusions</p> <p>We demonstrate the successful generation and empirical validation of a cell-specific knockout of AR from VE cells, and conclude that AR expression in VE cells is not essential for spermatogenesis or male fertility.</p

Innovative eLearning- und ePrüfungsanwendungen für die Aus-, Weiter- und Fortbildung in Medizin und Zahnmedizin

Matthies HK, Krückeberg J, von Jan U, Albrecht U-V. Innovative eLearning- und ePrüfungsanwendungen für die Aus-, Weiter- und Fortbildung in Medizin und Zahnmedizin. In: Vornberger O, ed. Teaching Trends: neue Konzepte des Technologie-Einsatzes in der Hochschule. Osnabrücker Beiträge zum medienbasierten Lernen. Vol 3. Osnabrück: epOs-media; 2012: 50-54

Getting around Antarctica: new high-resolution mappings of the grounded and freely-floating boundaries of the Antarctic ice sheet created for the International Polar Year.

The boundary of grounded ice and the location of ice transitioning to a freely floating state are mapped at 15-m resolution around the entire continent of Antarctica. These data products are produced by participants of the International Polar Year project ASAID using customized software combining Landsat-7 imagery and ICESat laser altimetry. The grounded ice boundary is 53 610 km long; 74% of it abuts to floating ice shelves or outlet glaciers, 19% is adjacent to open or sea-ice covered ocean, and 7% of the boundary are land terminations with bare rock. Elevations along each line are selected from 6 candidate digital elevation models: two created from the input ICESat laser altimetry and Landsat data, two from stereo satellite imagery, and two from compilations of primarily radar altimetry. Elevation selection and an assignment of confidence in the elevation value are based on agreement with ICESat elevation values and shape of the surface inferred from the Landsat imagery. Elevations along the freely-floating boundary (called the hydrostatic line) are converted to ice thicknesses by applying a firn-correction factor and a flotation criterion. The relationship between the seaward offset of the hydrostatic line from the grounding line only weakly matches a prediction based on beam theory. Airborne data are used to validate the technique of grounding line mapping, elevation selection and ice thickness derivation. The mapped products along with the customized software to generate them and a variety of intermediate products are available from the National Snow and Ice Data Center

Status of the down-regulated canine testis using two different GNRH agonist implants in comparison with the juvenile testis

Testicular function in the dog was down-regulated using two different GNRH agonist implants, with adult and juvenile testes serving as controls. Treatment resulted in an increased percentage of the interstitial area and decreased area of Leydig cell nuclei. Expression of StAR and the steroidogenic enzymes cytochrome P450 side-chain cleavage enzyme (P450scc, CYP11A1) and cytochrome P450 17α-hydroxylase-17,20-lyase (P450c17, CYP17A1) in Leydig cells was blocked at the mRNA and protein level, showing no differences between the two agonists. Staining for androgen receptor (AR) by immunohistochemistry was positive in Sertoli, Leydig and peritubular cells and some spermatogonia, with in situ hybridization confirming expression in Sertoli cells. At the mRNA level, expression of AR was not affected; however, translation was blocked (reduced percentage of AR-positive Sertoli cells), with the number of nuclei in basal position being decreased. In the juvenile testes, mRNA expression of StAR, CYP11A1 and CYP17A1 was higher compared with the other groups but distinctly lower for the AR. At the protein level, the expression was at the limit of detection for StAR; AR-positive Sertoli cells were not detected. Our observations show that the down-regulated testis is different from the juvenile one rather resembling the testicular status in seasonal breeders out of season