Pelatihan Training Circles 15 Repetisi 4 Set dan 10 Repetisi 6 Set terhadap Peningkatan Kekuatan Otot Perut Siswa Putri Kelas VIII SMP Negeri 2 Kuta Tahun Pelajaran 2017/2018

Berdasarkan pengamatan, prestasi cabang olahraga atletik maupun permainan sampai saat ini masih belum mampu menunjukkan hasil yang optimal hal ini karena siswa kurangnya mendapat pelatihan otot Perut, kurang menguasai teknik latihan otot Perut serta kurangnya minat dalam melatih otot Perut. Populasi dalam penelitian ini adalah siswa putri kelas VIII SMP Negeri 2 Kuta berjumlah 58 orang dan seluruhnya digunakan sebagai sempel dengan teknik populasi study yang meneliti seluruh subjek penelititan. Alat ukur yang digunakan adalah tes perbuatan dengan melakukan baring duduk (sit-up) selama 30 detik. Analisis data yang dipergunakan adalah metode analisis statistic dengan rumus t-tes. Berdasarkan analisis data diperoleh t-tes kelompok eksperimen I sebesar 5,742 sedangkan t-tabel didapat sebesar 2,048 dengan taraf signifikansi 5% dandb = 28. Demikian juga pada kelompok eksperimen II diperoleh t-tes sebesar 7,061 sedangkan t-tabel didapat sebesar 2,048 dengan taraf signifikansi 5% dandb = 28. Perbedaan kelompok eksperimen I dan II di peroleh t-tes sebesar 1,318 sedangkan t-tabel didapat sebesar 2,000 dengan taraf signifikansi 5% dan db = 56. Berdasarkan hasil penelitian ini, dapat disimpulkan  bahwa eksperimen pertama dan eksperimen kedua sama-sama mempunyai pengaruh yang signifikan terhadap peningkatan kekuatan otot Perut siswa putrid kelas VIII SMP Negeri 2 Kuta. Sehingga eksperimen pertama dan eksperimen kedua, hipotesis nol ditolak dan hipotesis alternative diterima. Untuk perbedaan pengaruh eksperimen pertama dan eksperimen kedua, hipotesis nol diterima dan hipotesis alternative ditolak. Karena kedua bentuk pelatihan tidak ada perbedaan pengaruh yang signifikan maka aplikasi di lapangan dapat dipergunakan salah satu atau kedua bentuk pelatihan training circles 15 repetisi 4 set dan 10 repetisi 6 set terhadap peningkatan kekuatan otot Perut siswa putrid kelas VIII SMP Negeri 2 Kuta

COMBINATION OF MOMORDICA CHARANTIA AND PHYLLANTHUS AMARUS FOR HEPATOPROTECTIVE ACTIVITY IN ETHANOL AND ANTI-TUBERCULAR DRUGS INDUCED HEPATOTOXICITY IN RATS

Objective: To evaluate the synergistic protective effect of Momordica charantia and Phyllanthus amarus combination (MC+PA) of doses 200 and 400 mg/kg on the liver in different experimental models of hepatotoxicity. Methods: The hepatoprotective activity was evaluated in ethanol and anti-tubercular drugs (isoniazid-INH, rifampicin-RIF) induced hepatotoxicity models. Hepatotoxicity in both models was induced to all groups except the normal control. Intoxicated rats were treated with silymarin and various doses of MC+PA for 8 d in ethanol-induced and 21 d in INH+RIF induced hepatotoxicity models. At the completion of study, the biochemical markers and the anti-oxidant status (SOD and MDA) were measured and also the histopathological evaluation of the liver tissue was carried out. Results: Combination therapy remarkably reduced the elevated profile of the biochemical markers and thereby improved the anti-oxidant status, thus exhibiting the synergistic hepatoprotective effect when compared with the positive control group (p<0.001). Histopathological evaluation demonstrated that MC+PA decreased the liver damage significantly in comparison with the positive group. Conclusion: The current work suggests that the combined extract showed synergistic effects on ethanol and anti-tubercular drugs induced hepatotoxicity models by significantly decreasing the liver damage

Ascorbic Acid is an Abettor in Calcium Urolithiasis: An Experimental Study

Two sets of animal experiments using guinea pigs were planned to evaluate the effect of ascorbic acid supplementation on the lithogenic process. In the first set of experiments, 10, 40, and 60 mg doses of ascorbic acid/100g body weight/day were given for 105 days. Neither of the ascorbic acid doses given induced crystalluria, calcification or stone formation, thereby confirming our previous findings that ascorbic acid in the doses used by clinicians does not cause urolith formation. In the second set of experiments, ascorbic acid was supplemented in hypercalciuric (induced by calcium carbonate feeding) and hyperoxaluric (induced by sodium oxalate feeding) animals for 45 days. The results indicated that it exacerbated the calcification process in renal and bladder tissue

Synthesizing non-natural parts from natural genomic template

<p>Abstract</p> <p>Background</p> <p>The current knowledge of genes and proteins comes from 'naturally designed' coding and non-coding regions. It would be interesting to move beyond natural boundaries and make user-defined parts. To explore this possibility we made six non-natural proteins in <it>E. coli</it>. We also studied their potential tertiary structure and phenotypic outcomes.</p> <p>Results</p> <p>The chosen intergenic sequences were amplified and expressed using pBAD 202/D-TOPO vector. All six proteins showed significantly low similarity to the known proteins in the NCBI protein database. The protein expression was confirmed through Western blot. The endogenous expression of one of the proteins resulted in the cell growth inhibition. The growth inhibition was completely rescued by culturing cells in the inducer-free medium. Computational structure prediction suggests globular tertiary structure for two of the six non-natural proteins synthesized.</p> <p>Conclusion</p> <p>To our best knowledge, this is the first study that demonstrates artificial synthesis of non-natural proteins from existing genomic template, their potential tertiary structure and phenotypic outcome. The work presented in this paper opens up a new avenue of investigating fundamental biology. Our approach can also be used to synthesize large numbers of non-natural RNA and protein parts for useful applications.</p

Si nanoparticle interfaces in Si/SiO2 solar cell materials

Novel solar cell materials consisting of Si nanoparticles embedded in SiO2 layers have been studied using positron annihilation spectroscopy in Doppler broadening mode and photoluminescence. Two positron-trapping interface states are observed after high temperature annealing at 1100 °C. One of the states is attributed to the (SiO2/Si bulk) interface and the other to the interface between the Si nanoparticles and SiO2. A small reduction in positron trapping into these states is observed after annealing the samples in N2 atmosphere with 5% H2. Enhanced photoluminescence is also observed from the samples following this annealing step.Peer reviewe

Automated design of DNA origami

Peer reviewe

Identification of novel homozygous SLURP1 mutation in a Javanese family with Mal de Meleda

Background Mal de Meleda (OMIM# 248300; keratosis palmoplantaris transgrediens) is an autosomal recessive form of palmoplantar keratoderma, clinically characterized by sharp demarcated erythema and hyperkeratosis of the palms and soles that progress with age and extend to the dorsal aspects of the hands and feet. The mal de Meleda is caused by mutations in the SLURP1 gene that encodes secreted lymphocyte antigen 6/urokinasetype plasminogen receptor-related protein 1 (SLURP1). To date no reported cases from Indonesia. The aims of the study were to describe the typical features of mal de Meleda cases in a Javanese family in Indonesia and identify the mutation in the ARS B gene which encodes SLURP1. Patients and Methods In this study, three Javanese patients, siblings from nonconsanguineous nonaffected parents, presented with classical symptoms of mal de Meleda. Genetic analysis screening SLURP1 gene was conducted for the specimens from the patients and other family members. Results A novel homozygous three-nucleotide deletion in exon 3, i.e. c.271-273TCTdel, was identified in the patients. Subcloning and sequencing revealed both parents (I.2 and I.3) and one of the father’s siblings (I.1) carry heterozygous c.271-273TCTdel, while the other father’s sibling (I.2), the mother’s sister (I.4), and a healthy control matched the ethnicity of the family, showing normal sequence of the entire SLURP1. Conclusion This is the first mal de Meleda case of Javanese ethnicity to be documented, and the unique mutation has not previously been reported. The finding supports the notion that despite the rarity, SLURP1 mutation causing mal de Meleda is ubiquitous