The Half-lives of 132^{132}La and 135^{135}La

The half-lives of $^{135}$La and $^{132}$La were determined via gamma spectroscopy and high-precision ionization chamber measurements. The results are 18.930(6) h for $^{135}$La and 4.59(4) h for $^{132}$La compared to the previously compiled values of 19.5(2) h and 4.8(2) h, respectively. The new results represent an improvement in the precision and accuracy of both values. These lanthanum isotopes comprise a medically interesting system with positron emitter $^{132}$La and Auger electron emitter $^{135}$La forming a matched pair for internal diagnostics and therapeutics. The precise half-lives are necessary for proper evaluation of their value in medicine and for a more representative tabulation of nuclear data.Comment: 11 pages, 3 figure

METHODOLOGICAL CONSIDERATIONS FOR COMPARISONS OF UPPER EXTREMITY EMG BETWEEN INDIVIDUALS WITH AND WITHOUT PARAPLEGIA

This study compared normalization methods for surface electromyography (sEMG) for comparing individuals with (Para) and without (AB) paraplegia. Participants (Para, n=7, AB, n=11) performed 4 minutes of arm-cycling at several submaximal intensities, and an incremental maximal test to exhaustion, while sEMG of the right biceps brachii was recorded. This study analyzed sEMG at two intensities: rate of perceived exertion (RPE) 13 and at 60 W, with four methods of normalization: non-normalized, against a maximal voluntary contraction (MVIC), against a rate of perceived exertion (RPE) 9, and against the max test. Using submaximal exercise intensity based on RPE or power output will affect the results when comparing sEMG of Para and AB groups, regardless of which normalization method is used to inspect the data

Biomimetic Catalysis with Immobilised Organometallic Ruthenium(II) Complexes: Substrate and Regioselective Transfer Hydrogenation of Ketones

Chloro-(h6-arene) complexes of Ru(II) with N-sulfonyl-1,2-ethylenediamine ligands that have one or two styrene side chains were synthesized and characterized. The chloro ligand was substituted with a diphenylphosphinato ligand and the resulting organometallic complexes are transition state analogs for the Ru-catalyzed transfer hydrogenation of benzophenone. Following the protocol of mol. imprinting, these complexes were copolymd. with ethylene glycol dimethacrylate (EGDMA) in the presence of a porogen. The polymers were ground and sieved, and the phosphinato ligand was substituted with a chloro ligand, thus generating a shape-selective cavity in close proximity to the catalytically active metal center. When tested for their ability to catalyze the redn. of benzophenone, the imprinted polymers showed a significantly higher activity (up to a factor of seven) than control polymers without cavities. Out of a mixt. of seven different arom. and aliph. ketones, benzophenone was preferentially reduced when the imprinted polymer was used. Also, the specificity of the catalyst for diaryl ketones was confirmed in a reaction with a bifunctional substrate, 4-acetyl-benzophenone; the diaryl ketone was reduced faster with the imprinted catalyst than the acetyl group. The opposite regioselectivity was obsd. with the control polymer. Both the activity and the selectivity of the imprinted catalysts are dependent on how the Ru complexes are attached to the polymer backbone. A double connection proved to give superior results

The In-Feed Antibiotic Carbadox Induces Phage Gene Transcription in the Swine Gut Microbiome

Carbadox is a quinoxaline-di-N-oxide antibiotic fed to over 40% of young pigs in the United States that has been shown to induce phage DNA transduction in vitro; however, the effects of carbadox on swine microbiome functions are poorly understood. We investigated the in vivo longitudinal effects of carbadox on swine gut microbial gene expression (fecal metatranscriptome) and phage population dynamics (fecal dsDNA viromes). Microbial metagenome, transcriptome, and virome sequences were annotated for taxonomic inference and gene function by using FIGfam (isofunctional homolog sequences) and SEED subsystems databases. When the beta diversities of microbial FIGfam annotations were compared, the control and carbadox communities were distinct 2 days after carbadox introduction. This effect was driven by carbadox-associated lower expression of FIGfams (n = 66) related to microbial respiration, carbohydrate utilization, and RNA metabolism (q \u3c 0.1), suggesting bacteriostatic or bactericidal effects within certain populations. Interestingly, carbadox treatment caused greater expression of FIGfams related to all stages of the phage lytic cycle 2 days following the introduction of carbadox (q ≤0.07), suggesting the carbadox-mediated induction of prophages and phage DNA recombination. These effects were diminished by 7 days of continuous carbadox in the feed, suggesting an acute impact. Additionally, the viromes included a few genes that encoded resistance to tetracycline, aminoglycoside, and beta-lactam antibiotics but these did not change in frequency over time or with treatment. The results show decreased bacterial growth and metabolism, prophage induction, and potential transduction of bacterial fitness genes in swine gut bacterial communities as a result of carbadox administration

Neodymium-140 DOTA-LM3:Evaluation of an <i>In Vivo</i> Generator for PET with a Non-Internalizing Vector

140Nd (t1/2 = 3.4 days), owing to its short-lived positron emitting daughter 140Pr (t1/2 = 3.4 min), has promise as an in vivo generator for positron emission tomography (PET). However, the electron capture decay of 140Nd is chemically disruptive to macrocycle-based radiolabeling, meaning that an in vivo redistribution of the daughter 140Pr is expected before positron emission. The purpose of this study was to determine how the delayed positron from the de-labeled 140Pr affects preclinical imaging with 140Nd. To explore the effect, 140Nd was produced at CERN-ISOLDE, reacted with the somatostatin analogue, DOTA-LM3 (1,4,7,10- tetraazacyclododecane, 1,4,7- tri acetic acid, 10- acetamide N - p-Cl-Phecyclo(d-Cys-Tyr-d-4-amino-Phe(carbamoyl)-Lys-Thr-Cys)d-Tyr-NH2) and injected into H727 xenograft bearing mice. Comparative pre- and post-mortem PET imaging at 16 h postinjection was used to quantify the in vivo redistribution of 140Pr following 140Nd decay. The somatostatin receptor-positive pancreas exhibited the highest tissue accumulation of 140Nd-DOTA-LM3 (13% ID/g at 16 h) coupled with the largest observed redistribution rate, where 56 ± 7% (n = 4, mean ± SD) of the in situ produced 140Pr washed out of the pancreas before decay. Contrastingly, the liver, spleen, and lungs acted as strong sink organs for free 140Pr3+. Based upon these results, we conclude that 140Nd imaging with a non-internalizing vector convolutes the biodistribution of the tracer with the accumulation pattern of free 140Pr. This redistribution phenomenon may show promise as a probe of the cellular interaction with the vector, such as in determining tissue dependent internalization behavior