A Time-Variant Value-Focused Methodology for Supporting Pre-Acquisition

Military operations are dynamic in nature, as time-dependent requirements or adversary actions can contribute to differing levels of mission performance among systems. Future military operations commonly use multi-criteria decision analysis techniques that rely on value-focused thinking (VFT) to analyze and ultimately rank alternatives during the Analysis of Alternatives phase of the acquisition process. Traditional VFT approaches are not typically employed with the intention of analyzing time-variant performance of alternatives. In this research, a holistic approach towards integrating fundamental practices such as VFT, systems architecture, and modeling and simulation is used to analyze time-dependent data outputs of an alternative’s performance within an operational environment. Incorporating this approach prior to Milestone A of the acquisition process allows for the identification of time-based capability gaps and additional dynamic analysis of possible alternatives that can be implemented as a flexible means of assessment. As part of this research, the pre-acquisition methodology is implemented with a hypothetical multi-domain Intelligence, Surveillance, and Reconnaissance mission in order to exemplify multiple time-dependent analysis possibilities

Image Analysis to Determine Intramuscular Fat in Muscle

The area of intramuscular fat in Holstein steer longissimus was determined using an image analyzing system. Slaughter weights of 500, 636 and 773 kg differed (p \u3c 0.05) for intramuscular fat area, marbling score, and ether extractable lipid . Repeated measurements of intramuscular fat area in a given section showed high accuracy . However, comparing two sections from the same sample, there was often a large difference in fat content between the sections. Fat content determined by the imaging system was correlated significantl y with marbling score (r = 0.49) and ether extractable lipid (r = 0.34). Sampling is critical, and in order to obtain a high correlation several samples would be required from each muscle

Shared autonomic pathways connect bone marrow and peripheral adipose tissues across the central neuraxis

Bone marrow adipose tissue (BMAT) is increased in both obesity and anorexia. This is unique relative to white adipose tissue (WAT), which is generally more attuned to metabolic demand. It suggests that there may be regulatory pathways that are common to both BMAT and WAT and also those that are specific to BMAT alone. The central nervous system (CNS) is a key mediator of adipose tissue function through sympathetic adrenergic neurons. Thus, we hypothesized that central autonomic pathways may be involved in BMAT regulation. To test this, we first quantified the innervation of BMAT by tyrosine hydroxylase (TH) positive nerves within the metaphysis and diaphysis of the tibia of B6 and C3H mice. We found that many of the TH+ axons were concentrated around central blood vessels in the bone marrow. However, there were also areas of free nerve endings which terminated in regions of BMAT adipocytes. Overall, the proportion of nerve-associated BMAT adipocytes increased from proximal to distal along the length of the tibia (from ~3-5 to ~14-24%), regardless of mouse strain. To identify the central pathways involved in BMAT innervation and compare to peripheral WAT, we then performed retrograde viral tract tracing with an attenuated pseudorabies virus (PRV) to infect efferent nerves from the tibial metaphysis (inclusive of BMAT) and inguinal WAT (iWAT) of C3H mice. PRV positive neurons were identified consistently from both injection sites in the intermediolateral horn of the spinal cord, reticular formation, rostroventral medulla, solitary tract, periaqueductal gray, locus coeruleus, subcoeruleus, Barrington\u27s nucleus, and hypothalamus. We also observed dual-PRV infected neurons within the majority of these regions. Similar tracings were observed in pons, midbrain, and hypothalamic regions from B6 femur and tibia, demonstrating that these results persist across mouse strains and between skeletal sites. Altogether, this is the first quantitative report of BMAT autonomic innervation and reveals common central neuroanatomic pathways, including putative command neurons, involved in coordinating multiple aspects of sympathetic output and facilitation of parallel processing between bone marrow/BMAT and peripheral adipose tissue

Metallic Coulomb Blockade Thermometry down to 10 mK and below

We present an improved nuclear refrigerator reaching 0.3 mK, aimed at microkelvin nanoelectronic experiments, and use it to investigate metallic Coulomb blockade thermometers (CBTs) with various resistances R. The high-R devices cool to slightly lower T, consistent with better isolation from the noise environment, and exhibit electron-phonon cooling ~ T^5 and a residual heat-leak of 40 aW. In contrast, the low-R CBTs display cooling with a clearly weaker T-dependence, deviating from the electronphonon mechanism. The CBTs agree excellently with the refrigerator temperature above 20 mK and reach a minimum-T of 7.5 +/- 0.2 mK.Comment: 3 pages, 3 (color) figure

Deregulation of the endogenous C/EBPβ LIP isoform predisposes to tumorigenesis

Two long and one truncated isoforms (termed LAP*, LAP, and LIP, respectively) of the transcription factor CCAAT enhancer binding protein beta (C/EBPbeta) are expressed from a single intronless Cebpb gene by alternative translation initiation. Isoform expression is sensitive to mammalian target of rapamycin (mTOR)-mediated activation of the translation initiation machinery and relayed through an upstream open reading frame (uORF) on the C/EBPbeta mRNA. The truncated C/EBPbeta LIP, initiated by high mTOR activity, has been implied in neoplasia, but it was never shown whether endogenous C/EBPbeta LIP may function as an oncogene. In this study, we examined spontaneous tumor formation in C/EBPbeta knockin mice that constitutively express only the C/EBPbeta LIP isoform from its own locus. Our data show that deregulated C/EBPbeta LIP predisposes to oncogenesis in many tissues. Gene expression profiling suggests that C/EBPbeta LIP supports a pro-tumorigenic microenvironment, resistance to apoptosis, and alteration of cytokine/chemokine expression. The results imply that enhanced translation reinitiation of C/EBPbeta LIP promotes tumorigenesis. Accordingly, pharmacological restriction of mTOR function might be a therapeutic option in tumorigenesis that involves enhanced expression of the truncated C/EBPbeta LIP isoform. KEY MESSAGE: Elevated C/EBPbeta LIP promotes cancer in mice. C/EBPbeta LIP is upregulated in B-NHL. Deregulated C/EBPbeta LIP alters apoptosis and cytokine/chemokine networks. Deregulated C/EBPbeta LIP may support a pro-tumorigenic microenvironment