A reference human induced pluripotent stem cell line for large-scale collaborative studies

Human induced pluripotent stem cell (iPSC) lines are a powerful tool for studying development and disease, but the considerable phenotypic variation between lines makes it challenging to replicate key findings and integrate data across research groups. To address this issue, we sub-cloned candidate human iPSC lines and deeply characterized their genetic properties using whole genome sequencing, their genomic stability upon CRISPR-Cas9-based gene editing, and their phenotypic properties including differentiation to commonly used cell types. These studies identified KOLF2.1J as an all-around well-performing iPSC line. We then shared KOLF2.1J with groups around the world who tested its performance in head-to-head comparisons with their own preferred iPSC lines across a diverse range of differentiation protocols and functional assays. On the strength of these findings, we have made KOLF2.1J and its gene-edited derivative clones readily accessible to promote the standardization required for large-scale collaborative science in the stem cell field

Hybrid meta-heuristic algorithms for a supply chain network considering different carbon emission regulations using big data characteristics

Big data (BD) approach has significantly impacted on the development and expansion of supply chain network management and design. The available problems in the supply chain network (SCN) include production, distribution, transportation, ordering, and inventory holding problems. These problems under the BD environment are challenging and considerably affect the efficiency of the SCN. The drastic environmental and regulatory changes around the world and the rising concerns about carbon emissions have increased the awareness of customers regarding the carbon footprint of the products they are consuming. This has enforced supply chain managers to change strategies to reframe carbon emissions. The decisions such as an optimization of the suitable network of the proper lot sizes can play a crucial role in minimizing the whole carbon emissions in the SCN. In this paper, a new integrated production–transportation–ordering–inventory holding problem for SCN is developed. In this regard, a mixed-integer nonlinear programming (MINLP) model in the multi-product, multi-level, and multi-period SCN is formulated based on the minimization of the total costs and the related cost of carbon emissions. The research also uses a chance-constrained programming approach. The proposed model needs a range of real-time parameters from capacities, carbon caps, and costs. These parameters along with the various sizes of BD, namely velocity, variety, and volume, have been illustrated. A lot-sizing policy along with carbon emissions is also provided in the proposed model. One of the important contributions of this paper is the three various carbon regulation policies that include carbon capacity-and-trade, the strict capacity on emission, and the carbon tax on emissions in order to assess the carbon emissions. As there is no benchmark available in the literature, this study contributes toward this aspect by proposing two hybrid novel meta-heuristics (H-1) and (H-2) to optimize the large-scale problems with the complex structure containing BD. Hence, a generated random dataset possessing the necessary parameters of BD, namely velocity, variety, and volume, is provided to validate and solve the suggested model. The parameters of the proposed algorithms are calibrated and controlled using the Taguchi approach. In order to evaluate hybrid algorithms and find optimal solutions, the study uses 15 randomly generated data examples having necessary features of BD and T test significance. Finally, the effectiveness and performance of the presented model are analyzed by a set of sensitivity analyses. The outcome of our study shows that H-2 is of higher efficiency