Circular Dichroism of Partially Purified Cytochrome P450 from Rabbit Liver Microsomes

The heme-related circular dichroic bands of solubilized cytochrome P450 from rabbit liver microsomes and some of its liganded derivatives were measured in the Soret region. All P450 derivatives exhibit negative circular dichroic bands in the region of the Soret absorption. The wavelengths of the dichroic bands and their ellipticities vary with ligand substitution and the oxidation state of the iron. The results are compared with CD-data from other hemoproteins and discussed with respect to stereochemical conclusions concerning the geometry and the physicochemical character of the vicinity of the heme group with regard to results obtained from other studies

Evidence of the Existence of a High Spin Low Spin Equilibrium in Liver Microsomal Cytochrome P450, and its Role in the Enzymatic Mechanism

In rabbit liver microsomal cytochrome P450 a high spin (S = = 5/2) low spin (S = 1/2) equilibrium has been proved to exist by recording temperature difference spectra in the Soret and in the visible region of the absorption spectrum of solubilized cytochrome P450. In the presence of type II substrates the predominantly low spin state of cytochrome P450 is maintained, only a very small shift to lower spin is observed. Ligands of the heme iron, such as cyanide and imidazole, pr9duce a pure low spin state and therefore in the presence of these ligands no temperature difference spectra can be obtained. In the presence of type I substrate, however, the spin equilibrium is shifted to the high spin state. The extent of this shift (1) depends on specific properties of the substrate and (2) it is generally relatively small, up to about 80/o in the case of substrates investigated so far

Determination of the laser-induced damage threshold of polymer optical fibers

Investigating the properties of manufactured polymer optical fibers is essential to determine proper areas of application. Using pulsed laser radiation, especially with respect to laser activity in optical fibers, the maximum acceptable transmittable energy without inducing damage is of particular interest. Therefore, this work is related to laser-induced damage in polymer optical fibers at a wavelength of 532 nm and a pulse duration of 7.3 ns. In particular, the influence of the coupling condition on the transmittable pulse energy and the damage behavior applying an R-on-1 test procedure are analyzed in this study. The obtained results give information about the long-Term behavior and will be used to optimize the manufacturing process. © COPYRIGHT SPI

Miniaturized thin-film filters to connect multiple self-written waveguides

Self-written waveguides (SWWs) have been well investigated within the last decades. In most cases, they are used as low-loss coupling structures, i.e., to connect buried optical structures in photonic integrated circuits. In this work, we extend the field of possible applications for SWWs by embedding a novel thin-film filter to split the beam and connect multiple output ports simultaneously. The multilayer design of the dielectric filter can be customized to enable its application as a dichroic beamsplitter for photonic networks. The embedded thin-film filter was characterized in detail and used to connect an additional optical sensing element, which is also based on SWWs, to demonstrate its usability for measurement of physical quantities

Laser-induced degradation and damage morphology in polymer optical fibers

The radiation of pulsed laser systems can generate changes in various materials. On the one hand, these modifications can be used for a variety of applications i.e. laser welding, cutting and many more [1]. The precision and quality depends on the material and laser parameters. On the other hand, material changes are not always desired in other applications. When using optical materials such as optical fibers as a light guide or as a sensor, laser-induced damage effects inside the fiber are to be prevented to ensure constant light guidance and the reliable monitoring of a desired parameter. Therefore, investigations for quality assurance need to be performed. For this reason, this work investigates laserinduced damage in polymer optical fibers (POF) using a nanosecond pulsed laser system at a wavelength of 532 nm. The impact of different laser and fiber parameters on the long-term degradation behavior is observed. In addition, the overall degradation behavior as well as the knowledge gained by analyzing the damage morphology and distribution will be used to obtain a better understanding of the damage mechanisms

Substrate-free miniaturized thin-film filters for single-element coarse wavelength division multiplexing fibers

We have created high-precision, miniaturized, substrate-free filters, based on ion beam sputtering on a sacrificial substrate. The sacrificial layer is cost efficient and environmentally friendly and can be dissolved using only water. We demonstrate an improved performance compared to filters on thin polymer layers from the same coating run. With these filters, a single-element coarse wavelength division multiplexing transmitting device for telecommunication applications can be realized by inserting the filter between fiber ends

Evidence of the Existence of a High Spin Low Spin Equilibrium in Liver Microsomal Cytochrome P450, and its Role in the Enzymatic Mechanism

In rabbit liver microsomal cytochrome P450 a high spin (S = = 5/2) low spin (S = 1/2) equilibrium has been proved to exist by recording temperature difference spectra in the Soret and in the visible region of the absorption spectrum of solubilized cytochrome P450. In the presence of type II substrates the predominantly low spin state of cytochrome P450 is maintained, only a very small shift to lower spin is observed. Ligands of the heme iron, such as cyanide and imidazole, pr9duce a pure low spin state and therefore in the presence of these ligands no temperature difference spectra can be obtained. In the presence of type I substrate, however, the spin equilibrium is shifted to the high spin state. The extent of this shift (1) depends on specific properties of the substrate and (2) it is generally relatively small, up to about 80/o in the case of substrates investigated so far

Circular Dichroism of Partially Purified Cytochrome P450 from Rabbit Liver Microsomes

The heme-related circular dichroic bands of solubilized cytochrome P450 from rabbit liver microsomes and some of its liganded derivatives were measured in the Soret region. All P450 derivatives exhibit negative circular dichroic bands in the region of the Soret absorption. The wavelengths of the dichroic bands and their ellipticities vary with ligand substitution and the oxidation state of the iron. The results are compared with CD-data from other hemoproteins and discussed with respect to stereochemical conclusions concerning the geometry and the physicochemical character of the vicinity of the heme group with regard to results obtained from other studies