58 research outputs found
Interferon-γ suppresses S100A4 transcription independently of apoptosis or cell cycle arrest
Validation of podocalyxin-like protein as a biomarker of poor prognosis in colorectal cancer
Prognostic and predictive significance of podocalyxin-like protein expression in pancreatic and periampullary adenocarcinoma
The Role of Podocalyxin in Health and Disease
Podocalyxin, a sialomucin most closely related to CD34 and endoglycan, is expressed
by kidney podocytes, hematopoietic progenitors, vascular endothelia, and
a subset of neurons; aberrant expression has recently been implicated in a range
of cancers. Through interactions with several intracellular proteins and at least one
extracellular ligand, podocalyxin regulates both adhesion and cell morphology. In
the developing kidney, podocalyxin plays an essential role in the formation and
maintenance of podocyte foot processes, and its absence results in perinatal
lethality. Podocalyxin expression in the hematopoietic system correlates with cell
migration and the seeding of new hematopoietic tissues. In addition, it is abnormally
expressed in subsets of breast, prostate, liver, pancreatic, and kidney cancer
as well as leukemia. Strikingly, it is often associated with the most aggressive cases,
and it is likely involved in metastasis. Thus, a thorough investigation of the normal
activities of podocalyxin may facilitate the development of new cancer treatment
strategies.
J Am Soc Nephrol 20: 1669–1676, 2009. doi: 10.1681/ASN.200807078
Regulation of eosinophil-specific gene expression by a C/EBP-Ets complex and GATA-1.
The EOS47 antigen is an early and specific marker of eosinophil differentiation in the chicken haematopoietic system. To elucidate the transciptional events controlling commitment to the eosinophil lineage, we studied the regulation of the eosinophil-specific EOS47 promoter. This promoter is TATA-less, and binds trancription factors of the Ets, C/EBP, GATA and Myb families. These sites are contained within a 309 bp promoter fragment which is sufficient for specific high level transcription in an eosinophil cell line. Co-transfection experiments in Q2bn fibroblasts showed cooperative activation of the EOS47 proximal promoter by c-Myb, Ets-1/Fli-1, GATA-1 and C/EBPalpha. The Ets-1/Fli-1 and C/EBPalpha proteins were the most potent activators, and acted with high synergy through juxtaposed binding sites located approximately 60 bp upstream of the transcription start site. The Ets-1 and C/EBPalpha proteins were found to associate physically via their DNA-binding domains and to bind their combined binding site cooperatively. GATA-1 showed biphasic regulation of the EOS47 promoter, activating at low and repressing at high protein concentrations. These results demonstrate combinatorial activation of an eosinophil-specific promoter by ubiquitous and lineage-restricted haematopoietic transcription factors. They also indicate that direct interactions between C/EBPs and specific Ets family members, together with GATA-1, are important for eosinophil lineage determination
Podocalyxin: a marker of blasts in acute leukemia
Copyright 2005 American Society for Clinical Patholog
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