The Unusual Substrate Specificity of a Virulence Associated Serine Hydrolase from the Highly Toxic Bacterium, \u3cem\u3eFrancisella tularensis\u3c/em\u3e

Francisella tularensis is the causative agent of the highly, infectious disease, tularemia. Amongst the genes identified as essential to the virulence of F. tularensis was the proposed serine hydrolase FTT0941c. Herein, we purified FTT0941c to homogeneity and then characterized the folded stability, enzymatic activity, and substrate specificity of FTT0941c. Based on phylogenetic analysis, FTT0941c was classified within a divergent Francisella subbranch of the bacterial hormone sensitive lipase (HSL) superfamily, but with the conserved sequence motifs of a bacterial serine hydrolase. FTT0941c showed broad hydrolase activity against diverse libraries of ester substrates, including significant hydrolytic activity across alkyl ester substrates from 2 to 8 carbons in length. Among a diverse library of fluorogenic substrates, FTT0941c preferred α-cyclohexyl ester substrates, matching with the substrate specificity of structural homologues and the broad open architecture of its modeled binding pocket. By substitutional analysis, FTT0941c was confirmed to have a classic catalytic triad of Ser115, His278, and Asp248 and to remain thermally stable even after substitution. Its overall substrate specificity profile, divergent phylogenetic homology, and preliminary pathway analysis suggested potential biological functions for FTT0941c in diverse metabolic degradation pathways in F. tularensis

Radiative corrections to the lattice gluon action for highly improved staggered quarks (HISQ) and the effect of such corrections on the static potential

We perform a perturbative calculation of the influence of dynamical HISQ fermions on the perturbative improvement of the gluonic action in the same way as we have previously done for asqtad fermions. We find the fermionic contributions to the radiative corrections in the Luescher-Weisz gauge action to be somewhat larger for HISQ fermions than for asqtad. Using one-loop perturbation theory as a test, we estimate that omission of the fermion-induced radiative corrections in dynamical asqtad simulations will give a measurable effect. The one-loop result gives a systematic shift of about -0.6% in (r_1/a) on the coarsest asqtad improved staggered ensembles. This is the correct sign and magnitude to explain the scaling violations seen in Phi_B on dynamical lattice ensembles.Comment: 10 pages, 5 figures. Minor corrections suggested by refere

The monopole mass in the three-dimensional Georgi-Glashow model

We study the three-dimensional Georgi-Glashow model to demonstrate how magnetic monopoles can be studied fully non-perturbatively in lattice Monte Carlo simulations, without any assumptions about the smoothness of the field configurations. We examine the apparent contradiction between the conjectured analytic connection of the `broken' and `symmetric' phases, and the interpretation of the mass (i.e., the free energy) of the fully quantised 't Hooft-Polyakov monopole as an order parameter to distinguish the phases. We use Monte Carlo simulations to measure the monopole free energy and its first derivative with respect to the scalar mass. On small volumes we compare this to semi-classical predictions for the monopole. On large volumes we show that the free energy is screened to zero, signalling the formation of a confining monopole condensate. This screening does not allow the monopole mass to be interpreted as an order parameter, resolving the paradox.Comment: 12 pages, 7 figures, uses revtex. Minor changes made to the text to match with the published version at http://link.aps.org/abstract/PRD/v65/e12500

Extent of regretted sexual intercourse among young teenagers in Scotland: a cross sectional survey

No abstract available

Comparative performance of diffused junction indium phosphide solar cells

A comparison is made between indium phosphide solar cells whose p-n junctions were processed by open tube capped diffusion, and closed tube uncapped diffusion, of sulfur into Czochralski grown p-type substrates. Air mass zero, total area, efficiencies ranged from 10 to 14.2 percent, the latter value attributed to cells processed by capped diffusion. The radiation resistance of these latter cells was slightly better, under 1 MeV electron irradiation. However, rather than being process dependent, the difference in radiation resistance could be attributed to the effects of increased base dopant concentration. In agreement with previous results, both cells exhibited radiation resistance superior to that of gallium arsenide. The lowest temperature dependency of maximum power was exhibited by the cells prepared by open tube capped diffusion. Contrary to previous results, no correlation was found between open circuit voltage and the temperature dependency of Pmax. It was concluded that additional process optimization was necessary before concluding that one process was better than another

Voltage controlling mechanisms in low resistivity silicon solar cells: A unified approach

An experimental technique capable of resolving the dark saturation current into its base and emitter components is used as the basis of an analysis in which the voltage limiting mechanisms were determined for a variety of high voltage, low resistivity silicon solar cells. The cells studied include the University of Florida hi-low emitter cell, the NASA and the COMSAT multi-step diffused cells, the Spire Corporation ion-implanted emitter cell, and the University of New South Wales MINMIS and MINP cells. The results proved to be, in general, at variance with prior expectations. Most surprising was the finding that the MINP and the MINMIS voltage improvements are due, to a considerable extent, to a previously unrecognized optimization of the base component of the saturation current. This result is substantiated by an independent analysis of the material used to fabricate these devices

Ground-layer wavefront reconstruction from multiple natural guide stars

Observational tests of ground layer wavefront recovery have been made in open loop using a constellation of four natural guide stars at the 1.55 m Kuiper telescope in Arizona. Such tests explore the effectiveness of wide-field seeing improvement by correction of low-lying atmospheric turbulence with ground-layer adaptive optics (GLAO). The wavefronts from the four stars were measured simultaneously on a Shack-Hartmann wavefront sensor (WFS). The WFS placed a 5 x 5 array of square subapertures across the pupil of the telescope, allowing for wavefront reconstruction up to the fifth radial Zernike order. We find that the wavefront aberration in each star can be roughly halved by subtracting the average of the wavefronts from the other three stars. Wavefront correction on this basis leads to a reduction in width of the seeing-limited stellar image by up to a factor of 3, with image sharpening effective from the visible to near infrared wavelengths over a field of at least 2 arc minutes. We conclude that GLAO correction will be a valuable tool that can increase resolution and spectrographic throughput across a broad range of seeing-limited observations.Comment: 25 pages, 8 figures, to be published in Astrophys.

The Two Phases of Galaxy Formation

Cosmological simulations of galaxy formation appear to show a two-phase character with a rapid early phase at z>2 during which in-situ stars are formed within the galaxy from infalling cold gas followed by an extended phase since z<3 during which ex-situ stars are primarily accreted. In the latter phase massive systems grow considerably in mass and radius by accretion of smaller satellite stellar systems formed at quite early times (z>3) outside of the virial radius of the forming central galaxy. These tentative conclusions are obtained from high resolution re-simulations of 39 individual galaxies in a full cosmological context with present-day virial halo masses ranging from 7e11 M_sun h^-1 < M_vir < 2.7e13 M_sun h^-1 and central galaxy masses between 4.5e10 M_sun h^-1 < M_* < 3.6e11 M_sun h^-1. The simulations include the effects of a uniform UV background, radiative cooling, star formation and energetic feedback from SNII. The importance of stellar accretion increases with galaxy mass and towards lower redshift. In our simulations lower mass galaxies ($M_* < 9e10 M_sun h^-1) accrete about 60 per cent of their present-day stellar mass. High mass galaxy ($M_* > 1.7e11 M_sun h^-1) assembly is dominated by accretion and merging with about 80 per cent of the stars added by the present-day. In general the simulated galaxies approximately double their mass since z=1. For massive systems this mass growth is not accompanied by significant star formation. The majority of the in-situ created stars is formed at z>2, primarily out of cold gas flows. We recover the observational result of archaeological downsizing, where the most massive galaxies harbor the oldest stars. We find that this is not in contradiction with hierarchical structure formation. Most stars in the massive galaxies are formed early on in smaller structures, the galaxies themselves are assembled late.Comment: 13 pages, 13 figures, accepted for publication in Ap

The potential therapeutic effects of creatine supplementation on body composition and muscle function in cancer

Low muscle mass in individuals with cancer has a profound impact on quality of life and independence and is associated with greater treatment toxicity and poorer prognosis. Exercise interventions are regularly being investigated as a means to ameliorate treatment-related adverse effects, and nutritional/supplementation strategies to augment adaptations to exercise are highly valuable. Creatine (Cr) is a naturally-occurring substance in the human body that plays a critical role in energy provision during muscle contraction. Given the beneficial effects of Cr supplementation on lean body mass, strength, and physical function in a variety of clinical populations, there is therapeutic potential in individuals with cancer at heightened risk for muscle loss. Here, we provide an overview of Cr physiology, summarize the evidence on the use of Cr supplementation in various aging/clinical populations, explore mechanisms of action, and provide perspectives on the potential therapeutic role of Cr in the exercise oncology setting