172 research outputs found
Molecular Maps in Cereals: Methodology and Progress
Cereals provide for our major food crops, and therefore have been a subject
of detailed genetic and cytogenetic studies during major part of the last
century. These studies led to the preparation of linkage maps, which were
also assigned to individual chromosomes, thus leading to the construction of
chromosome maps in all major cereals. In some cases, the availability of
cytogenetic stocks (e.g. deletion stocks in bread wheat) also allowed
construction of physical maps. In the past, a major limitation in the
construction of genetic maps has been the non-availability of mutants for
majority of individual genes, so that only handful of genes could be mapped.
However, during 1980s, the availability of molecular markers and the high
level of DNA polymorphism, which they detect, led to renewed emphasis on
genetic and physica.......
Characterization of Hypertension Risk Factors at the Committee on Temporary Shelter
Introduction: The health of homeless populations is at risk due to a high prevalence of undiagnosed hypertension (HTN) and cardiovascular disease (CVD). The interaction of housing and socioeconomic status with the risk factors for HTN and CVD remains unclear. Prevention of HTN through a healthy diet, exercise, adequate sleep, and avoidance of tobacco has been well described, but financial limitations and competing priorities for shelter and food make blood pressure (BP) control difficult for this population. By characterizing the risk factors and awareness of hypertension within the homeless population at the Committee on Temporary Shelter Daystation (COTS) in Burlington, Vermont, we may be able to identify promising avenues for therapeutic intervention.https://scholarworks.uvm.edu/comphp_gallery/1226/thumbnail.jp
Single nucleotide polymorphisms in rye (Secale cereale L.): discovery, frequency, and applications for genome mapping and diversity studies
To elucidate the potential of single nucleotide
polymorphism (SNP) markers in rye, a set of 48
barley EST (expressed sequence tag) primer pairs was
employed to amplify from DNA prepared from five
rye inbred lines. A total of 96 SNPs and 26 indels
(insertionβdeletions) were defined from the sequences
of 14 of the resulting amplicons, giving an estimated
frequency of 1 SNP per 58 bp and 1 indel per 214 bp
in the rye transcriptome. A mean of 3.4 haplotypes per
marker with a mean expected heterozygosity of 0.66
were observed. The nucleotide diversity index (p) was
estimated to be in the range 0.0059β0.0530. To improve
assay cost-effectiveness, 12 of the 14 SNPs were
converted to a cleaved amplified polymorphic sequence
(CAPS) format. The resulting 12 SNP loci
mapped to chromosomes 1R, 3R, 4R, 5R, 6R, and 7R,
at locations consistent with their known map positions
in barley. SNP genotypic data were compared with
genomic simple sequence repeat (SSR) and EST-derived
SSR genotypic data collected from the same
templates. This showed a broad equivalence with respect
to genetic diversity between these different data
types
Radiation monitoring using imaging plate technology: A case study of leaves affected by the Chernobyl nuclear power plant and JCO criticality accidents
This paper describes the use of a photostimulable phosphor screen imaging technique to detect radioactive contamination in the leaves of wormwood (Artemisia vulgaris L) and fern (Dryopteris filix-max CL. Schoff) plants affected by the Chernobyl nuclear power plant accident. The imaging plate technology is well known for many striking performances in two-dimensional radiation detection. Since imaging plate comprises an integrated detection system, it has been extensively applied to surface contamination distribution studies. In this study, plant samples were collected from high- and low-contaminated areas of Ukraine and Belarus, which were affected due to the Chernobyl accident and exposed to imaging technique. Samples from the highly contaminated areas revealed the highest photo-stimulated luminescence on the imaging plate. Moreover, the radio nuclides detected in the leaves by gamma and beta ray spectroscopy were 137Cs and 90Sr, respectively. Additionally, in order to assess contamination, a comparison was also made with leaves of plants affected during the JCO criticality accident in Japan. Based on the results obtained, the importance of imaging plate technology in environmental radiation monitoring has been suggested
ΠΠΠΠ«Π¨ΠΠΠΠ ΠΠ€Π€ΠΠΠ’ΠΠΠΠΠ‘Π’Π ΠΠ‘ΠΠΠΠ¬ΠΠΠΠΠΠΠ― ΠΠΠΠΠΠ ΠΠ‘Π’ΠΠΠΠΠ§ΠΠ‘ΠΠΠΠ ΠΠ ΠΠΠΠΠ― ΠΠ Π Π ΠΠΠΠ Π‘ΠΠΠ’ΠΠΠ ΠΠ ΠΠΠΠ‘Π’ΠΠΠ«
The paper presents a comparative analysis pertaining to cutting mono-crystal silicon by a diamond wheel with internal cutting-edge (DWIC) and a multi-stranded tool. It has been shown that in accordance to the output percentage of good plates usage of multi-stranded cutting method is on a par with DWIC; and in accordance to an actual consumption of silicon per one plate the first method exceeds the wheel cutting method. The process of multi-stranded cutting method is characterized by high accuracy, reproducibility, productivity and ensures significant saving of high-priced mono-crystal silicon.ΠΡΠ΅Π΄ΡΡΠ°Π²Π»Π΅Π½Ρ ΡΡΠ°Π²Π½ΠΈΡΠ΅Π»ΡΠ½ΡΠ΅ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΡ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΡ ΡΠ΅Π·ΠΊΠΈ ΠΌΠΎΠ½ΠΎΠΊΡΠΈΡΡΠ°Π»Π»ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΊΡΠ΅ΠΌΠ½ΠΈΡ Π°Π»ΠΌΠ°Π·Π½ΡΠΌ ΠΊΡΡΠ³ΠΎΠΌ Ρ Π²Π½ΡΡΡΠ΅Π½Π½Π΅ΠΉ ΡΠ΅ΠΆΡΡΠ΅ΠΉ ΠΊΡΠΎΠΌΠΊΠΎΠΉ (ΠΠΠΠ ) ΠΈΒ ΠΌΠ½ΠΎΠ³ΠΎΠΏΡΠΎΠ²ΠΎΠ»ΠΎΡΠ½ΠΎΠΉ ΡΠ΅Π·ΠΊΠΈ. ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ ΠΏΠΎ ΠΏΡΠΎΡΠ΅Π½ΡΡ Π²ΡΡ
ΠΎΠ΄Π° Π³ΠΎΠ΄Π½ΡΡ
ΠΏΠ»Π°ΡΡΠΈΠ½ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΌΠ΅ΡΠΎΠ΄Π° ΠΌΠ½ΠΎΠ³ΠΎΠΏΡΠΎΠ²ΠΎΠ»ΠΎΡΠ½ΠΎΠΉ ΡΠ΅Π·ΠΊΠΈ Π½Π΅ ΡΡΡΡΠΏΠ°Π΅Ρ ΠΌΠ΅ΡΠΎΠ΄Ρ ΡΠ΅Π·ΠΊΠΈ ΠΊΡΡΠ³Π°ΠΌΠΈ ΠΠΠΠ , Π° ΠΏΠΎ ΡΠ°ΠΊΡΠΈΡΠ΅ΡΠΊΠΎΠΌΡ ΡΠ°ΡΡ
ΠΎΠ΄Ρ ΠΊΡΠ΅ΠΌΠ½ΠΈΡ Π½Π° ΠΎΠ΄Π½Ρ ΠΏΠ»Π°ΡΡΠΈΠ½Ρ ΠΏΡΠ΅Π²ΠΎΡΡ
ΠΎΠ΄ΠΈΡ ΠΌΠ΅ΡΠΎΠ΄ Π΄ΠΈΡΠΊΠΎΠ²ΠΎΠΉ ΡΠ΅Π·ΠΊΠΈ. ΠΡΠΎΡΠ΅ΡΡ ΠΌΠ½ΠΎΠ³ΠΎΠΏΡΠΎΠ²ΠΎΠ»ΠΎΡΠ½ΠΎΠΉ ΡΠ΅Π·ΠΊΠΈ Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΠ·ΡΠ΅ΡΡΡ Π²ΡΡΠΎΠΊΠΎΠΉ ΡΠΎΡΠ½ΠΎΡΡΡΡ, Π²ΠΎΡΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄ΠΈΠΌΠΎΡΡΡΡ, ΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄ΠΈΡΠ΅Π»ΡΠ½ΠΎΡΡΡΡ ΠΈ ΠΎΠ±Π΅ΡΠΏΠ΅ΡΠΈΠ²Π°Π΅Ρ ΡΡΡΠ΅ΡΡΠ²Π΅Π½Π½ΡΡ ΡΠΊΠΎΠ½ΠΎΠΌΠΈΡ Π΄ΠΎΡΠΎΠ³ΠΎΡΡΠΎΡΡΠ΅Π³ΠΎ ΠΌΠΎΠ½ΠΎΠΊΡΠΈΡΡΠ°Π»Π»ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΊΡΠ΅ΠΌΠ½ΠΈΡ.
Single nucleotide polymorphisms in rye (Secale cereale L.): discovery, frequency, and applications for genome mapping and diversity studies
Abscisic Acid Flux Alterations Result in Differential Abscisic Acid Signaling Responses and Impact Assimilation Efficiency in Barley under Terminal Drought Stress
Abscisic acid (ABA) is a central player in plant responses to drought stress. How variable levels of ABAabscisic acid under short-term versus long-term drought stress impact assimilation and growth in crops is unclear. We addressed this through comparative analysis, using two elite breeding lines of barley (Hordeum vulgare) that show senescence or stay-green phenotype under terminal drought stress and by making use of transgenic barley lines that express Arabidopsis (Arabidopsis thaliana) 9-cis-epoxycarotenoid dioxygenase (AtNCED6) coding sequence or an RNA interference (RNAi) sequence of ABA 8β²-hydroxylase under the control of a drought-inducible barley promoter. The high levels of ABA and its catabolites in the senescing breeding line under long-term stress were detrimental for assimilate productivity, whereas these levels were not perturbed in the stay-green type that performed better. In transgenic barley, drought-inducible AtNCED expression afforded temporal control in ABA levels such that the ABA levels rose sooner than in wild-type plants but also subsided, unlike as in the wild type , to near-basal levels upon prolonged stress treatment due to down-regulation of endogenous HvNCED genes. Suppressing of ABA catabolism with the RNA interference approach of ABA 8β²-hydroxylase caused ABA flux during the entire period of stress. These transgenic plants performed better than the wild type under stress to maintain a favorable instantaneous water use efficiency and better assimilation. Gene expression analysis, protein structural modeling, and protein-protein interaction analyses of the members of the PYRABACTIN RESISTANCE1/PYRABACTIN RESISTANCE1-LIKE/REGULATORY COMPONENT OF ABA RECEPTORS, TYPE 2C PROTEIN PHOSPHATASE Sucrose non-fermenting1-related protein kinase2, and ABA-INSENSITIVE5/ABA-responsive element binding factor family identified specific members that could potentially impact ABA metabolism and stress adaptation in barley
Π€ΠΠΠΠΠ-Π₯ΠΠΠΠ§ΠΠ‘ΠΠΠ Π‘ΠΠΠΠ‘Π’ΠΠ Π£ΠΠ¬Π’Π ΠΠΠΠ‘ΠΠΠ Π‘ΠΠ«Π₯ ΠΠΠ ΠΠ¨ΠΠΠ ΠΠ ΠΠΠΠΠ―
Experimental investigations of ultradispersed silicon powder produced by mechanical grinding are conducted. The process of scaling powders using mechanochemical grinding was studied. It was found that the dimension of crystallites of the silicon powder determines their physical and chemical properties. Finely divided powders of silicon with a particle size β€10 nm easily decompose water at room temperature into hydrogen and oxygen, they are easily oxidized by air oxygen and actively interact with hydroxides and acids with the release of significant amount of heat. The rate of saturation of micro- and ultradispersed powders with hydrogen at a constant rate of flow in the flow system depends on the temperature of the hydrogen hydrogenation process. It was found that the hydride formed at a low temperature (β€573 K) is in an equilibrium state when it is being hydrogenated in a flow reactor. The treated powder passes into a metastable state with the termination of the thermal action after hydrogenation. Hydrogen, entering the volume of micro- and ultradispersed silicon powder in the temperature range 373β723 K due to the diffusion process, can react not only with silicon atoms to form hydrides of the SiH2 type, but also with hydrides of less active radicals of the type of Si2H4, Si2H6. The hydrogenation process has two distinct stages. It is shown that the temperature of the transition between the stages is determined by the dispersion of micro- and ultradispersed silicon powders and by the structure of hydrides on the particle surface. As a result, it is established that the optimum temperature of hydrogenation should be taken at a temperature of 623 K, at which the maximum mobility of silicon atoms is reached during the formation of hydride. Sharp decrease in the hydrogen content is observed at a temperature above 723 K for the Si2H4 (SiH2).ΠΡΠΎΠ²Π΅Π΄Π΅Π½Ρ ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°Π»ΡΠ½ΡΠ΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΡΠ»ΡΡΡΠ°Π΄ΠΈΡΠΏΠ΅ΡΡΠ½ΠΎΠ³ΠΎ ΠΏΠΎΡΠΎΡΠΊΠ° ΠΊΡΠ΅ΠΌΠ½ΠΈΡ, ΠΏΠΎΠ»ΡΡΠ΅Π½Π½ΠΎΠ³ΠΎ ΠΏΡΡΠ΅ΠΌ ΠΏΠΎΠΌΠΎΠ»Π° ΠΏΠΎΠ»ΡΠΏΡΠΎΠ²ΠΎΠ΄Π½ΠΈΠΊΠΎΠ²ΠΎΠ³ΠΎ ΠΌΠΎΠ½ΠΎΠΊΡΠΈΡΡΠ°Π»Π»ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΊΡΠ΅ΠΌΠ½ΠΈΡ, ΠΈΠ·ΡΡΠ΅Π½ ΠΏΡΠΎΡΠ΅ΡΡ ΠΌΠ°ΡΡΡΠ°Π±ΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΠΏΠΎΡΠΎΡΠΊΠΎΠ² Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ ΠΌΠ΅Ρ
Π°Π½ΠΎΡ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΈΠ·ΠΌΠ΅Π»ΡΡΠ΅Π½ΠΈΡ. Π£ΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ, ΡΡΠΎ ΡΠ°Π·ΠΌΠ΅ΡΠ½ΠΎΡΡΡ ΠΊΡΠΈΡΡΠ°Π»Π»ΠΈΡΠΎΠ² ΠΏΠΎΡΠΎΡΠΊΠΎΠ² ΠΊΡΠ΅ΠΌΠ½ΠΈΡ ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΠ΅Ρ ΠΈΡ
ΡΠΈΠ·ΠΈΠΊΠΎ-Ρ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΡΠ²ΠΎΠΉΡΡΠ²Π°. Π£Π»ΡΡΡΠ°Π΄ΠΈΡΠΏΠ΅ΡΡΠ½ΡΠ΅ ΠΏΠΎΡΠΎΡΠΊΠΈ ΠΊΡΠ΅ΠΌΠ½ΠΈΡ Ρ ΡΠ°Π·ΠΌΠ΅ΡΠΎΠΌ ΡΠ°ΡΡΠΈΡ β€10 Π½ΠΌ Π»Π΅Π³ΠΊΠΎ ΡΠ°Π·Π»Π°Π³Π°ΡΡ Π²ΠΎΠ΄Ρ ΠΏΡΠΈ ΠΊΠΎΠΌΠ½Π°ΡΠ½ΠΎΠΉ ΡΠ΅ΠΌΠΏΠ΅ΡΠ°ΡΡΡΠ΅ Π½Π° Π²ΠΎΠ΄ΠΎΡΠΎΠ΄ ΠΈ ΠΊΠΈΡΠ»ΠΎΡΠΎΠ΄, ΠΎΠ½ΠΈ Π»Π΅Π³ΠΊΠΎ ΠΎΠΊΠΈΡΠ»ΡΡΡΡΡ ΠΊΠΈΡΠ»ΠΎΡΠΎΠ΄ΠΎΠΌ Π²ΠΎΠ·Π΄ΡΡ
Π° ΠΈ Π°ΠΊΡΠΈΠ²Π½ΠΎ Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΡΡΡ Ρ Π³ΠΈΠ΄ΡΠΎΠΎΠΊΡΠΈΠ΄Π°ΠΌΠΈ ΠΈ ΠΊΠΈΡΠ»ΠΎΡΠ°ΠΌΠΈ Ρ Π²ΡΠ΄Π΅Π»Π΅Π½ΠΈΠ΅ΠΌ Π·Π½Π°ΡΠΈΡΠ΅Π»ΡΠ½ΠΎΠ³ΠΎ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π° ΡΠ΅ΠΏΠ»Π°. Π‘ΠΊΠΎΡΠΎΡΡΡ Π½Π°ΡΡΡΠ΅Π½ΠΈΡ ΠΌΠΈΠΊΡΠΎ- ΠΈ ΡΠ»ΡΡΡΠ°Π΄ΠΈΡΠΏΠ΅ΡΡΠ½ΡΡ
ΠΏΠΎΡΠΎΡΠΊΠΎΠ² Π²ΠΎΠ΄ΠΎΡΠΎΠ΄ΠΎΠΌ ΠΏΡΠΈ ΠΏΠΎΡΡΠΎΡΠ½Π½ΠΎΠΉ ΡΠΊΠΎΡΠΎΡΡΠΈ ΠΏΠΎΡΠΎΠΊΠ° Π² ΠΏΡΠΎΡΠΎΡΠ½ΠΎΠΉ ΡΠΈΡΡΠ΅ΠΌΠ΅ Π·Π°Π²ΠΈΡΠΈΡ ΠΎΡ ΡΠ΅ΠΌΠΏΠ΅ΡΠ°ΡΡΡΡ ΠΏΡΠΎΡΠ΅ΡΡΠ° Π³ΠΈΠ΄ΡΠΈΡΠΎΠ²Π°Π½ΠΈΡ Π²ΠΎΠ΄ΠΎΡΠΎΠ΄Π°. Π£ΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ, ΡΡΠΎ Π³ΠΈΠ΄ΡΠΈΠ΄, ΠΎΠ±ΡΠ°Π·ΡΡΡΠΈΠΉΡΡ ΠΏΡΠΈ Π½ΠΈΠ·ΠΊΠΎΠΉ ΡΠ΅ΠΌΠΏΠ΅ΡΠ°ΡΡΡΠ΅ (β€573 Π) Π½Π°Ρ
ΠΎΠ΄ΠΈΡΡΡ Π² ΡΠ°Π²Π½ΠΎΠ²Π΅ΡΠ½ΠΎΠΌ ΡΠΎΡΡΠΎΡΠ½ΠΈΠΈ ΠΏΡΠΈ Π³ΠΈΠ΄ΡΠΈΡΠΎΠ²Π°Π½ΠΈΠΈ Π² ΠΏΡΠΎΡΠΎΡΠ½ΠΎΠΌ ΡΠ΅Π°ΠΊΡΠΎΡΠ΅. Π‘ ΠΏΡΠ΅ΠΊΡΠ°ΡΠ΅Π½ΠΈΠ΅ΠΌ ΡΠ΅ΡΠΌΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ Π²ΠΎΠ·Π΄Π΅ΠΉΡΡΠ²ΠΈΡ ΠΏΠΎΡΠ»Π΅ Π³ΠΈΠ΄ΡΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΠΎΠ±ΡΠ°Π±ΠΎΡΠ°Π½Π½ΡΠΉ ΠΏΠΎΡΠΎΡΠΎΠΊ ΠΏΠ΅ΡΠ΅Ρ
ΠΎΠ΄ΠΈΡ Π² ΠΌΠ΅ΡΠ°ΡΡΠ°Π±ΠΈΠ»ΡΠ½ΠΎΠ΅ ΡΠΎΡΡΠΎΡΠ½ΠΈΠ΅. ΠΠΎΠ΄ΠΎΡΠΎΠ΄, ΠΏΠΎΡΡΡΠΏΠ°ΡΡΠΈΠΉ Π² ΠΎΠ±ΡΠ΅ΠΌ ΠΌΠΈΠΊΡΠΎ- ΠΈ ΡΠ»ΡΡΡΠ°Π΄ΠΈΡΠΏΠ΅ΡΡΠ½ΠΎΠ³ΠΎ ΠΏΠΎΡΠΎΡΠΊΠ° ΠΊΡΠ΅ΠΌΠ½ΠΈΡ Π² Π΄ΠΈΠ°ΠΏΠ°Π·ΠΎΠ½Π΅ ΡΠ΅ΠΌΠΏΠ΅ΡΠ°ΡΡΡ 373β723 Π Π·Π° ΡΡΠ΅Ρ Π΄ΠΈΡΡΡΠ·ΠΈΠΎΠ½Π½ΠΎΠ³ΠΎ ΠΏΡΠΎΡΠ΅ΡΡΠ°, ΠΌΠΎΠΆΠ΅Ρ ΡΠ΅Π°Π³ΠΈΡΠΎΠ²Π°ΡΡ Π½Π΅ ΡΠΎΠ»ΡΠΊΠΎ Ρ Π°ΡΠΎΠΌΠ°ΠΌΠΈ ΠΊΡΠ΅ΠΌΠ½ΠΈΡ Ρ ΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ Π³ΠΈΠ΄ΡΠΈΠ΄ΠΎΠ² ΡΠΈΠΏΠ° SiH2, Π½ΠΎ ΠΈ Ρ Π³ΠΈΠ΄ΡΠΈΠ΄Π°ΠΌΠΈ ΠΌΠ΅Π½Π΅Π΅ Π°ΠΊΡΠΈΠ²Π½ΡΡ
ΡΠ°Π΄ΠΈΠΊΠ°Π»ΠΎΠ² ΡΠΈΠΏΠ° Si2H4, Si2H6. ΠΡΠΎΡΠ΅ΡΡ Π³ΠΈΠ΄ΡΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΠΈΠΌΠ΅Π΅Ρ Π΄Π²Π΅ ΡΠ²Π½ΠΎ Π²ΡΡΠ°ΠΆΠ΅Π½Π½ΡΠ΅ ΡΡΠ°Π΄ΠΈΠΈ. ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ ΡΠ΅ΠΌΠΏΠ΅ΡΠ°ΡΡΡΠ° ΠΏΠ΅ΡΠ΅Ρ
ΠΎΠ΄Π° ΠΌΠ΅ΠΆΠ΄Ρ ΡΡΠ°Π΄ΠΈΡΠΌΠΈ ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΠ΅ΡΡΡ Π΄ΠΈΡΠΏΠ΅ΡΡΠ½ΠΎΡΡΡΡ ΠΌΠΈΠΊΡΠΎ- ΠΈ ΡΠ»ΡΡΡΠ°Π΄ΠΈΡΠΏΠ΅ΡΡΠ½ΡΡ
ΠΏΠΎΡΠΎΡΠΊΠΎΠ² ΠΊΡΠ΅ΠΌΠ½ΠΈΡ ΠΈ ΡΡΡΡΠΊΡΡΡΠΎΠΉ Π³ΠΈΠ΄ΡΠΈΠ΄ΠΎΠ² Π½Π° ΠΏΠΎΠ²Π΅ΡΡ
Π½ΠΎΡΡΠΈ ΡΠ°ΡΡΠΈΡ. Π ΠΈΡΠΎΠ³Π΅ ΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ, ΡΡΠΎ Π·Π° ΠΎΠΏΡΠΈΠΌΠ°Π»ΡΠ½ΡΡ ΡΠ΅ΠΌΠΏΠ΅ΡΠ°ΡΡΡΡ Π³ΠΈΠ΄ΡΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΡΠ»Π΅Π΄ΡΠ΅Ρ ΠΏΡΠΈΠ½ΡΡΡ ΡΠ΅ΠΌΠΏΠ΅ΡΠ°ΡΡΡΡ 623 Π, ΠΏΡΠΈ ΠΊΠΎΡΠΎΡΠΎΠΉ Π΄ΠΎΡΡΠΈΠ³Π°Π΅ΡΡΡ ΠΌΠ°ΠΊΡΠΈΠΌΠ°Π»ΡΠ½Π°Ρ ΠΏΠΎΠ΄Π²ΠΈΠΆΠ½ΠΎΡΡΡ Π°ΡΠΎΠΌΠΎΠ² ΠΊΡΠ΅ΠΌΠ½ΠΈΡ ΠΏΡΠΈ ΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΠΈ Π³ΠΈΠ΄ΡΠΈΠ΄Π°. ΠΡΠΈ ΡΠ΅ΠΌΠΏΠ΅ΡΠ°ΡΡΡΠ΅ Π²ΡΡΠ΅ 723 Π Π΄Π»Ρ Π³ΠΈΠ΄ΡΠΈΠ΄Π° Si2H4 (SiH2) Π½Π°Π±Π»ΡΠ΄Π°Π΅ΡΡΡ ΡΠ΅Π·ΠΊΠΎΠ΅ ΡΠΌΠ΅Π½ΡΡΠ΅Π½ΠΈΠ΅ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΡ Π²ΠΎΠ΄ΠΎΡΠΎΠ΄Π°
Spatial Structure of <i>Yersinia pestis</i> Population Belonging to Altai Biovar, Subspecies central asiatica Acording to Genome-Wide Sequencing Data
The aim of the work was to conduct phylogenetic analysis of Y. pestis strains, biovar altaica ssp. central asiatica, isolated in Gorno-Altai high-mountain and Sailyugem natural plague foci on the territory of Russia and Mongolia in 1965β2020, by full-genome sequencing data.Materials and methods. To determine the population structure of the biovar altaica ssp. central asiatica, 34 whole genome sequences were used (including 20 Y. pestis strains of the biovar altaica, 18 of which were sequenced by us). To isolate DNA from the Y. pestis strains, a PureLink Genomic DNA Mini Kit (Invitrogen, USA) was applied. Sequencing of the nucleotide sequences of Y. pestis strains was carried out in Ion PGM system Lifetechnologies. The analysis and processing of the obtained data were performed with the help of Newblergs Assembler 2.6 and IonTorrent Suite software package, 3.4.2. The search for SNPs was performed using the Wombac 2.0 program. The Maximum Likelihood dendrogram was built in the PhyML 3.1. The dendrogram was visualized using the FigTree 1.4.3 software.Results and discussion. Based on the data of whole genome analysis, taking into account the 1871 revealed polymorphic nucleotides, the spatial structure of the biovar altaica ssp. central asiatica has been determined. It includes several phylogeographic branches: the Kurai-Tarkhatinskaya (cluster 0.PE4a-1) and the Ulandryk-Mongolian (0.PE4a-2), which is in agreement with the geographical regions of the isolation of strains forming these branches in the Altai Mountains. The Kurai-Tarkhatinskaya branch is further divided into the Kurai (sub-cluster 0.PE4a-1-1, formed by the strains of 2009β2018) and Tarkhatinskaya (subcluster 0.PE4a-1-2, formed by the strains of 2012β2020) sub-branches, while the Ulandryk-Mongolian branch of evolution is split into sub-branches represented by strains from the Ulandryk meso focus (sub-cluster 0.PE4a-2-2, strains 1965β2010) and the Sailyugem focus of Mongolia (sub-cluster 0.PE4a-2-1, strains 1964β1990)
- β¦