5-Acetyl-4-(4-methoxy­phen­yl)-6-methyl-3,4-dihydro­pyrimidin-2(1H)-one

In the title mol­ecule, C14H16N2O3, the heterocyclic ring adopts a flattened boat conformation, and the plane through its four coplanar atoms makes a dihedral angle of 89.65 (7)° with the benzene ring. The non-H atoms of the carbonyl, acetyl and methyl groups are nearly coplanar with the attached heterocyclic ring. Inter­molecular N—H⋯O and C—H⋯O hydrogen bonds are present in the crystal structure

5-Acetyl-4-(2-chloro­phen­yl)-6-methyl-3,4-dihydro­pyrimidin-2(1H)-one

In the title mol­ecule, C13H13ClN2O2, the heterocyclic ring adopts a flattened boat conformation with the plane through the four coplanar atoms making a dihedral angle of 89.16 (5)° with the benzene ring, which adopts an axial orientation. The carbonyl, acetyl and methyl groups each have an equatorial orientation. In the crystal structure, inter­molecular N—H⋯O hydrogen bonds lead to a tape motif. The H atoms of the methyl group at position 6 are disordered over two positions of opposite orientation

Novel approaches to whole sporozoite vaccination against malaria

AbstractThe parasitic disease malaria threatens more than 3 billion people worldwide, resulting in more than 200 million clinical cases and almost 600,000 deaths annually. Vaccines remain crucial for prevention and ultimately eradication of infectious diseases and, for malaria, whole sporozoite based immunization has been shown to be the most effective in experimental settings. In addition to immunization with radiation-attenuated sporozoites, chemoprophylaxis and sporozoites (CPS) is a highly efficient strategy to induce sterile protection in humans. Genetically attenuated parasites (GAP) have demonstrated significant protection in rodent studies, and are now being advanced into clinical testing. This review describes the existing pre-clinical and clinical data on CPS and GAP, discusses recent developments and examines how to transform these immunization approaches into vaccine candidates for clinical development

Ethyl 6-methyl-2-sulfanyl­idene-4-[4-(trifluoro­meth­yl)phen­yl]-1,2,3,4-tetra­hydro­pyrimidine-5-carboxyl­ate

The title compound, C15H15F3N2O2S, adopts a conformation with an intra­molecular C—H⋯π inter­action. The dihedral angles between the planes of the 4-(trifluoro­meth­yl)phenyl and ester groups with the plane of the six-membered tetra­hydro­pyrimidine ring are 81.8 (1) and 16.0 (1)°, respectively. In the crystal structure, inter­molecular N—H⋯S hydrogen bonds link pairs of mol­ecules into dimers and N—H⋯O inter­actions generate hydrogen-bonded mol­ecular chains along the crystallographic a axis

Ethyl 4-(1,3-benzodioxol-5-yl)-6-methyl-2-sulfanylidene-1,2,3,4-tetra­hydro­pyrimidine-5-carboxyl­ate

In the title compound, C15H16N2O4S, the dihedral angles between the planes of the benzodioxole and ester groups and the plane of the six-membered tetra­hydro­pyrimidine ring are 89.5 (1) and 20.2 (1)°, respectively. Inter­molecular N—H⋯S hydrogen bonds assemble the mol­ecules into dimers, which are further connected via N—H⋯O inter­actions into chains parallel to [010]. Weak C—H⋯S and C—H⋯π inter­actions enhance the stability of the crystal structure

Collective Excitation of Spatio-Spectrally Distinct Quantum Dots Enabled by Chirped Pulses

For a scalable photonic device producing entangled photons, it is desirable to have multiple quantum emitters in an ensemble that can be collectively excited, despite their spectral variability. For quantum dots, Rabi rotation, the most popular method for resonant excitation, cannot assure a universal, highly efficient excited state preparation, because of its sensitivity to the excitation parameters. In contrast, Adiabatic Rapid Passage (ARP), relying on chirped optical pulses, is immune to quantum dot spectral inhomogeneity. Here, we advocate the robustness of ARP for simultaneous excitation of the biexciton states of multiple quantum dots. For positive chirps, we find that there is also regime of phonon advantage that widens the tolerance range of spectral detunings. Using the same laser pulse we demonstrate the simultaneous excitation of energetically and spatially distinct quantum dots. Being able to generate spatially multiplexed entangled photon pairs is a big step towards the scalability of photonic devices

ERNST: Demonstrating Advanced Infrared Detection from a 12U CubeSat

The ERNST mission will demonstrate complex infrared detection capabilities using a 12U CubeSat platform. ERNST’s main payload is an advanced cryogenically-cooled infrared imager that implicates demanding requirements in terms of power demand, heat dissipation, and vibration response for a nanosatellite. The optical bench that integrates optics, a filter-wheel for switching between spectral bands, and the detector-cooler system has been additively designed and manufactured, giving it a bionic appearance and combined with a highly efficient radiator. An onboard radiation monitor and a COTS camera complete the mission payloads. The ERNST 12U platform is based on high-performance CubeSat subsystems for avionics, UHF, and X-band communication, attitude control, and power management. The commercial components are made compatible through a backplane solution. In-house developments include a fast DPU and an autonomous de-orbit dragsail. The platform provides 30 Watt (OAP) and \u3e6U payload volume. After comprehensive environmental and functional testing of the Engineering Model, the Flight Model is currently being integrated. Starting operations in February 2023, ERNST will verify early warning concepts and technology

A Tandem Mass Spectrometry Sequence Database Search Method for Identification of O-Fucosylated Proteins by Mass Spectrometry.

Thrombospondin type 1 repeats (TSRs), small adhesive protein domains with a wide range of functions, are usually modified with O-linked fucose, which may be extended to O-fucose-β1,3-glucose. Collision-induced dissociation (CID) spectra of O-fucosylated peptides cannot be sequenced by standard tandem mass spectrometry (MS/MS) sequence database search engines because O-linked glycans are highly labile in the gas phase and are effectively absent from the CID peptide fragment spectra, resulting in a large mass error. Electron transfer dissociation (ETD) preserves O-linked glycans on peptide fragments, but only a subset of tryptic peptides with low m/ z can be reliably sequenced from ETD spectra compared to CID. Accordingly, studies to date that have used MS to identify O-fucosylated TSRs have required manual interpretation of CID mass spectra even when ETD was also employed. In order to facilitate high-throughput, automatic identification of O-fucosylated peptides from CID spectra, we re-engineered the MS/MS sequence database search engine Comet and the MS data analysis suite Trans-Proteomic Pipeline to enable automated sequencing of peptides exhibiting the neutral losses characteristic of labile O-linked glycans. We used our approach to reanalyze published proteomics data from Plasmodium parasites and identified multiple glycoforms of TSR-containing proteins