Pre-weaning growth performance and carcass traits of Awassi and Chios-Awassi lambs

One hundred and three lambs (26 Chios-Awassi (CA) and 77 Awassi (AA)) were used to evaluate the effect of crossbreeding on growth performance, body dimensions and carcass characteristics. At weaning, 8 CA and 8 AA male lambs were randomly selected to examine carcass traits. Crossbred CA lambs were heavier at birth than their AA counterparts, while average daily gain (ADG) and weaning weights were not affected by genotype. CA lamb genotype had greater diagonal body length, hip height and heart girth than AA lambs. Hot and cold carcass weight, dressing percentage, body length, leg length, maximum shoulder width, and width behind shoulder were greater in CA lambs compared with AA lambs. AA lambs had significantly heavier fat tail and leg cut weights, while shoulder cut weights were heavier in the CA lambs. CA lambs had greater rib fat depth and tissue depth thickness than AA lambs. Longissimus muscle area was significantly greater in CA than in AA lambs. In conclusion, CA lambs had greater birth weights, body dimensions and dressing percentage than AA lambs. Additionally, aside from the tail and leg cuts, carcass components were heavier in CA crossbred lambs.Keywords: Carcass characteristics; crossbreeding; weaning weigh

Neosporosis in Sheep and Different Breeds of Goats from Southern Jordan: Prevalence and Risk Factors Analysis

A cross sectional study was performed to investigate the epidemiology of Neospora caninum infection in Southern Jordan. A total of 320 sheep and 300 goats from 38 and 24, sheep and goat flocks, respectively, were randomly sampled and assayed for presence of antibodies against N. caninum. A structured pre-tested questionnaire was administered to collect information on flocks' health and management. A multivariable logistic regression model was constructed to investigate risk factors associated with seropositivity to N. caninum. The individual true seroprevalence of N. caninum in sheep and goats was 4.3 and 5.7%, respectively. The sheep and goat flock level true seroprevalence for N. caninum was 45.8 and 48.7%, respectively. The logistic regression model revealed small herd size, having more than one dog and grazing in communal pastures as risk factors for N. caninum seropositivity in both sheep and goats. Damascus breed goats were more likely to be seropositive than goats of other breeds.A.M. Al-Majali, K.I. Jawasreh, H.A. Talafha and A.Q. Talafh

Investigation of scrotal circumference, testicular dimensions and semen characteristics of the vulnerable Arabian oryx (Oryx leucoryx): an approach supports the future use of artificial insemination

Data regarding semen collection and characteristics in Arabian oryx are not available so far. Therefore, the current study aimed to evaluate scrotal circumference, testicular dimension and semen characteristics in four age groups of this species. Eleven male Arabian oryx bulls were divided into four groups according to age: group 1: 3 years old (n = 2); group 2: 4 years old (n = 2); group 3: 5 years old (n = 2) and group 4: 10 years old (n = 3). After immobilisation, scrotal circumference, testicular dimensions, volume and weight and gonadosomatic index (GSI) were estimated for each animal. Semen was collected from each male on a weekly basis for 3 weeks. Immediately after collection, semen was evaluated for volume, pH, osmolality total motility (TM), forward progressive motilities (PM) using a phase microscope. Furthermore, TM, PM, progressive velocity (PV), curvilinear velocity (VCL) and pathway velocity (VAP) have been evaluated using computer-assisted sperm analyser (CASA). Sperm concentration was evaluated using a haemocytometer, whereas sperm morphology was detected using phase contrast microscopy and staining methods by eosin-nigrosin and Spermac stains. Sperm viability was assessed using eosin-nigrosin stain under light microscopy and Sybr14/PI stain under fluorescence microscopy. The current findings indicated a positive correlation between body weight versus scrotal circumference, testicular weight, testicular volume and GSI. However, age did not have a positive association with percentage of normal sperm in Arabian oryx. Conclusively, the current findings provide a valuable data for future use of artificial insemination, which will be crucial for propagation and conservation of Arabian oryx

Genotype and Allelic Frequencies of a Newly Identified Mutation Causing Blindness in Jordanian Awassi Sheep Flocks

A total of 423 blood samples were collected (during 2009 and 2010) from all the ram holdings at three major Jordanian governmental Awassi breeding stations (Al-Khanasry, Al-Mushairfa and Al-Fjaje) and two private flocks. All blood samples were screened for the presence of mutations at the CNGA3 gene (responsible for day blindness in Awassi sheep) using RFLP-PCR. The day blindness mutation was detected in all studied flocks. The overall allele and genotype frequencies of all studied flocks of the day blindness mutation were 0.088 and 17.49%, respectively. The genotype and allele frequencies were higher in station flocks than the farmer flocks (0.121, 24.15 and 0.012, 2.32, respectively). Al-Mushairfa and Al-Khanasry stations have the highest genotype and allele frequencies for the day blindness mutation that were 27.77, 30.00% and 0.14, 0.171, respectively. The investigated farmer flocks have low percentages (0.03, 5.88% at Al-Shoubak and 0.005 and 1.05%, at Al-Karak, respectively for genotype and allele frequencies) compared with the breeding stations. Ram culling strategy was applied throughout the genotyping period in order to gradually eradicate this newly identified day blindness mutation from Jordanian Breeding station, since they annually distribute a high percentage of improved rams to farmer’s flocks