Exploring the Pharmacological Potential of the Chemically Characterized Essential Oil from Clinopodium nepeta subsp. ascendens: A Combined In Vitro and In Silico Analysis

This thorough examination explores the various biological characteristics present in the essential oil derived from Clinopodium nepeta subsp. ascendens (CNEO), a subspecies previously unrecognized and indigenous to the eastern part of Morocco. This subspecies is distinguished from C. nepeta by the distinctive feature of having purple or pale pinkish-white flowers. The study initiates with a thorough scrutiny of the phytochemical composition of CNEO via gas chromatography-mass spectrometry (GC-MS), revealing a nuanced spectrum of 24 terpene compounds. Among these, noteworthy constituents such as linalyl acetate (23.28%), Trifluoroacetyl-α-terpineol (13.66%), camphor (13.28%), and menthol (9.22%) are identified. These compounds, acknowledged for their notable biological and pharmacological attributes, serve as focal points for subsequent analyses. Beyond compositional elucidation, the study systematically investigates the diverse biological activities of CNEO. The essential oil exhibits substantial antioxidant potential, as substantiated by robust total antioxidant capacity (TAC) of 315.07 μg AA/mg and effective inhibition of DPPH free radicals (IC50 = 112.97 ± 2.67 µg/ml). Demonstrating promising antibacterial efficacy against various strains, occasionally surpassing gentamicin, positions CNEO as a potential antibacterial agent. Equally notable antifungal efficacy, surpassing that of cycloheximide, with low minimum inhibitory concentrations (MIC) is obtained, underscoring its potent antifungal properties. The antidiabetic potential of CNEO manifests through significant inhibition of xanthine oxidase (IC50 = 30.82 ± 0.78 µg/ml), α-amylase (IC50 = 40.13 ± 1.60 µg/ml), and α-glucosidase (IC50 = 45.30 ± 0.69 µg/ml) activities, suggesting therapeutic prospects in glycemic regulation. Furthermore, the essential oil showcases compelling anti-tyrosinase activity (IC50 = 29.78 ± 1.01 µg/ml), indicating potential dermatoprotective applications in melanin regulation. The implications of these findings provide a robust foundation for future investigations, unraveling the full therapeutic potential of CNEO within medical, cosmetic, and industrial contexts

Bilan d'hospitalisation du service de dermatologie-v&#233n&3233rologie du CHU Ibn Sina Rabat Maroc

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