For the Love of Pete: An Orphan Train Story

Review of: "For the Love of Pete: An Orphan Train Story," by Ethel Barker

Rapid Cue-Specific Remodeling of the Nascent Axonal Proteome.

Axonal protein synthesis and degradation are rapidly regulated by extrinsic signals during neural wiring, but the full landscape of proteomic changes remains unknown due to limitations in axon sampling and sensitivity. By combining pulsed stable isotope labeling of amino acids in cell culture with single-pot solid-phase-enhanced sample preparation, we characterized the nascent proteome of isolated retinal axons on an unparalleled rapid timescale (5 min). Our analysis detects 350 basally translated axonal proteins on average, including several linked to neurological disease. Axons stimulated by different cues (Netrin-1, BDNF, Sema3A) show distinct signatures with more than 100 different nascent protein species up- or downregulated within the first 5 min followed by further dynamic remodeling. Switching repulsion to attraction triggers opposite regulation of a subset of common nascent proteins. Our findings thus reveal the rapid remodeling of the axonal proteomic landscape by extrinsic cues and uncover a logic underlying attraction versus repulsion

Dimensionality reduction for point feature SLAM problems with spherical covariance matrices

© 2014 Elsevier Ltd. All rights reserved. The main contribution of this paper is the dimensionality reduction for multiple-step 2D point feature based Simultaneous Localization and Mapping (SLAM), which is an extension of our previous work on one-step SLAM (Wang et al.; 2013). It has been proved that SLAM with multiple robot poses and a number of point feature positions as variables is equivalent to an optimization problem with only the robot orientations as variables, when the associated uncertainties can be described using spherical covariance matrices. This reduces the dimension of original problem from 3m+2n to m only (where m is the number of poses and n is the number of features). The optimization problem after dimensionality reduction can be solved numerically using the unconstrained optimization algorithms. While dimensionality reduction may not provide computational saving for all nonlinear optimization problems, for some SLAM problems we can achieve benefits such as improvement on time consumption and convergence. For the special case of two-step SLAM when the orientation information from odometry is not incorporated, an algorithm that can guarantee to obtain the globally optimal solution (in the maximum likelihood sense) is derived. Simulation and experimental datasets are used to verify the equivalence between the reduced nonlinear optimization problem and the original full optimization problem, as well as the proposed new algorithm for obtaining the globally optimal solution for two-step SLAM

JUMPING MODE ATOMIC FORCE MICROSCOPY ON GRANA MEMBRANES FROM SPINACH

FWN – Publicaties zonder aanstelling Universiteit Leide

A dynamic perspective on affect and creativity

10.5465/amj.2010.0894Academy of Management Journal562432-45

Type I and type II interferon responses in two human liver cell lines (Huh-7 and HuH6)

AbstractMost studies investigating the biology of Hepatitis C virus (HCV) have used the human hepatoma cell line Huh-7 or subclones thereof, as these are the most permissive cell lines for HCV infection and replication. Other cell lines also support replication of HCV, most notably the human hepatoblastoma cell line HuH6. HCV replication in cell culture is generally highly sensitive to interferons (IFNs) and differences in the IFN-mediated inhibition of virus replication may reflect alterations in the IFN-induced antiviral response inherent to different host cells. For example, HCV replication is highly sensitive to IFN-γ treatment in Huh-7, but not in HuH6 cells. In this study, we used microarray-based gene expression profiling to compare the response of Huh-7 and HuH6 cells to stimulation with IFN-α and IFN-γ. Furthermore, we determined whether the resistance of HCV replication in HuH6 cells can be linked to differences in the expression profile of IFN-regulated genes. Although both cells lines responded to IFNs with rapid changes in gene expression, thereby demonstrating functional type I and type II signaling pathways, differences were observed for a number of genes. Raw and normalized expression data have been deposited in GEO under accession number GSE68927

Новые языковые реальности употребления претерита в немецком гипотаксисе

Целью статьи являются обобщение и теоретическое обоснование нового употребления претерита в относительном значении в конкретных видах придаточных предложений с презенсом в главном предложении. В статье анализируются коннотативные нюансы относительного значения претерита, регулярность замены префекта-пассива и перфекта именного составного сказуемого с глаголом "sein" на претеритальные формы в гипотаксисе, а также особое место претерита в системе немецких временных форм.Метою статті є узагальнення та теоретичне обґрунтування нового вживання претериту у релятивному значенні у конкретних видах сурядно-підрядних речень з презентом у сурядному реченні. У статті подано аналіз конототивні нюанси претериту і регулярність зміни перфекту пасиву та іменного присудка з дієсловом "sein" на претеритальні форми у гіпотаксису, а також особливе місце претериту у системі німецьких часових форм.The article aims at generalization and theoretical Explanation of modern preterit usage in relative meaning in certain types of clauses, with the Present tense in the main clause. The article deals with the analysis of connotative component meanings of the relative preterit and the regularity of substitution of preterit forms in hypotaxes for the passive perfect tense and the perfect form of the nominal compound predicate with the verb 'sein' as well as the specificity of preterit in the system of German tenses

ACCOUNTING OF DAMPING IN THE EVALUATION AND SEISMIC STABILITY TASKS OF BUILDINGS AND STRUCTURES

It is proved that for the majority of building structures characterized by different damping elements. The goal of constructing matrix hysteretic damping that takes into account the internal friction in the material. The study is based on generalized hypothesis of E. S. Sorokin on the proportionality of the matrices of damping and stiffness elements of the structure. The methods of accounting for damping in the evaluation of the earthquake resistance of structures. The exact and approximate methods of decomposition of the motion equations for the mode shapes are considered. It is established that the empirical criterion of the approximate method applicability of accounting for damping and its refinement on the basis of the initial approximation can serve as the correlation coefficients of waveforms. It is shown that for non-proportional damping it is possible to approximate the decomposition of movements according to oscillations forms of non-damped system, if the forms are weakly correlated

The quantitative proteomes of human-induced pluripotent stem cells and embryonic stem cells

An in-depth proteomic comparison of human-induced pluripotent stem cells, and their parent fibroblast cells, with embryonic stem cells shows that the reprogramming process comprehensively remodels protein expression levels, creating cells that closely resemble natural stem cells