4,000 research outputs found
HST Snapshot Survey of Post-AGB Objects
The results from a Hubble Space Telescope (HST) snapshot survey of post-AGB
objects are shown. The aim of the survey is to complement existing HST images
of PPN and to connect various types of nebulosities with physical and chemical
properties of their central stars. Nebulosities are detected in 15 of 33
sources. Images and photometric and geometric measurements are presented. For
sources with nebulosities we see a morphological bifurcation into two groups,
DUPLEX and SOLE, as previous studies have found. We find further support to the
previous results suggesting that this dichotomy is caused by a difference in
optical thickness of the dust shell. The remaining 18 sources are classified as
stellar post-AGB objects, because our observations indicate a lack of
nebulosity. We show that some stellar sources may in fact be DUPLEX or SOLE
based on their infrared colors. The cause of the differences among the groups
are investigated. We discuss some evidence suggesting that high progenitor-mass
AGB stars tend to become DUPLEX post-AGB objects. Intermediate progenitor-mass
AGB stars tend to be SOLE post-AGB objects. Most of the stellar sources
probably have low mass progenitors and do not seem to develop nebulosities
during the post-AGB phase and therefore do not become planetary nebulae.Comment: 21 pages, 11 figure
Visual product architecture modelling for structuring data in a PLM system
Part 8: Formalization for PLMInternational audienceThe goal of this paper is to determine the role of a product architecture model to support communication and to form the basis for developing and maintaining information of product structures in a PLM system. This paper contains descriptions of a modelling tool to represent a product architecture in a company to support the development of a family of products, as well as the reasons leading to the use of the specific model and its terminology. The fundamental idea for using the architecture model is that an improved understanding of the whole product system, will lead to better decision making. Moreover, it is discussed how the sometimes intangible elements and phenomena within an architecture model can be visually modeled in order to form the basis for a data model in a PLM system
Proton Motive Force-Dependent Hoechst 33342 Transport by the ABC Transporter LmrA of Lactococcus lactis
The fluorescent compound Hoechst 33342 is a substrate for many multidrug resistance (MDR) transporters and is widely used to characterize their transport activity. We have constructed mutants of the adenosine triphosphate (ATP) binding cassette (ABC)-type MDR transporter LmrA of Lactococcus lactis that are defective in ATP hydrolysis. These mutants and wild-type LmrA exhibited an atypical behavior in the Hoechst 33342 transport assay. In membrane vesicles, Hoechst 33342 transport was shown to be independent of the ATPase activity of LmrA, and it was not inhibited by orthovanadate but sensitive to uncouplers that collapse the proton gradient and to N,N'-dicyclohexylcarbodiimide, an inhibitor of the F0F1-ATPase. In contrast, transport of Hoechst 33342 by the homologous, heterodimeric MDR transporter LmrCD showed a normal ATP dependence and was insensitive to uncouplers of the proton gradient. With intact cells, expression of LmrA resulted in an increased rate of Hoechst 33342 influx while LmrCD caused a decrease in the rate of Hoechst 33342 influx. Cellular toxicity assays using a triple knockout strain, i.e., L. lactis ΔlmrA ΔlmrCD, demonstrate that expression of LmrCD protects cells against the growth inhibitory effects of Hoechst 33342, while in the presence of LmrA, cells are more susceptible to Hoechst 33342. Our data demonstrate that the LmrA-mediated Hoechst 33342 transport in membrane vesicles is influenced by the transmembrane pH gradient due to a pH-dependent partitioning of Hoechst 33342 into the membrane.
Routine versus on demand removal of the syndesmotic screw; a protocol for an international randomised controlled trial (RODEO-trial)
Background: Syndesmotic injuries are common and their incidence is rising. In case of surgical fixation of the syndesmosis a metal syndesmotic screw is used most often. It is however unclear whether this screw needs to be removed routinely after the syndesmosis has healed. Traditionally the screw is removed after six to 12 weeks as it is thought to hamper ankle functional and to be a source of pain. Some studies however suggest this is only the case in a minority of patients. We therefore aim to investigate the effect of retaining the syndesmotic screw on functional outcome. Design: This is a pragmatic international multicentre randomised controlled trial in patients with an acute syndesmotic injury for which a metallic syndesmotic screw was placed. Patients will be randomised to either routine removal of the syndesmotic screw or removal on demand. Primary outcome is functional recovery at 12 months measured with the Olerud-Molander Score. Secondary outcomes are quality of life, pain and costs. In total 194 patients will be needed to demonstrate non-inferiority between the two interventions at 80% power and a significance level of 0.025 including 15% loss to follow-up. Discussion: If removal on demand of the syndesmotic screw is non-inferior to routine removal in terms of functional outcome, this will offer a strong argument to adopt this as standard practice of care. This means that patients will not have to undergo a secondary procedure, leading to less complications and subsequent lower costs.Peer reviewe
Pulsations and Long-Term Light Variability of Three Candidates to Protoplanetary Nebulae
We present new photometric data and analysis of the long-duration UBV
photoelectric observations for three candidates to protoplanetary objects -
F-supergiants with IR-excesses located at large galactic latitudes, IRAS
18095+2704, IRAS 19386+0155, and IRAS 19500-1709. All three stars have revealed
quasiperiodic low-amplitude variabilities caused by pulsations observed against
the long-term trends of brightnesses. For IRAS 18095+2704=V887 Her we have
found a pulsation period of 109 days and a linear trend of brightness under the
constant colours if being averaged over the year timescale. The light curve of
IRAS 19386+0155=V1648 Aql over 2000-2008 can be approximated by a wave with a
main period of 102 days which is modulated by close frequency, with a period of
98 days, that results in brightness oscillations with a variable amplitude.
V1648 Aql has also shown synchronous reddening together with a persistent rise
of brightness in the V-band. IRAS 19500-1709=V5112 Sgr experiences irregular
pulsations with the periods of 39 and 47 days. The long-term component of the
variability of V5112 Sgr may be related to the binary character of this star.Comment: 11 pages, 6 figures, accepted for publication in Pis'ma Astron. Z
Formation of Structure in Snowfields: Penitentes, Suncups, and Dirt Cones
Penitentes and suncups are structures formed as snow melts, typically high in
the mountains. When the snow is dirty, dirt cones and other structures can form
instead. Building on previous field observations and experiments, this work
presents a theory of ablation morphologies, and the role of surface dirt in
determining the structures formed. The glaciological literature indicates that
sunlight, heating from air, and dirt all play a role in the formation of
structure on an ablating snow surface. The present work formulates a
mathematical model for the formation of ablation morphologies as a function of
measurable parameters. The dependence of ablation morphologies on weather
conditions and initial dirt thickness are studied, focusing on the initial
growth of perturbations away from a flat surface. We derive a single-parameter
expression for the melting rate as a function of dirt thickness, which agrees
well with a set of measurements by Driedger. An interesting result is the
prediction of a dirt-induced travelling instability for a range of parameters.Comment: 28 pages, 13 figure
Generation of Variants in Listeria monocytogenes Continuous-Flow Biofilms Is Dependent on Radical-Induced DNA Damage and RecA-Mediated Repair
The food-borne pathogen Listeria monocytogenes is a Gram-positive microaerophilic facultative anaerobic rod and the causative agent of the devastating disease listeriosis. L. monocytogenes is able to form biofilms in the food processing environment. Since biofilms are generally hard to eradicate, they can function as a source for food contamination. In several occasions biofilms have been identified as a source for genetic variability, which potentially can result in adaptation of strains to food processing or clinical conditions. However, nothing is known about mutagenesis in L. monocytogenes biofilms and the possible mechanisms involved. In this study, we showed that the generation of genetic variants was specifically induced in continuous-flow biofilms of L. monocytogenes, but not in static biofilms. Using specific dyes and radical inhibitors, we showed that the formation of superoxide and hydroxyl radicals was induced in continuous-flow biofilms, which was accompanied with in an increase in DNA damage. Promoter reporter studies showed that recA, which is an important component in DNA repair and the activator of the SOS response, is activated in continuous-flow biofilms and that activation was dependent on radical-induced DNA damage. Furthermore, continuous-flow biofilm experiments using an in-frame recA deletion mutant verified that RecA is required for induced generation of genetic variants. Therefore, we can conclude that generation of genetic variants in L. monocytogenes continuous-flow biofilms results from radical-induced DNA damage and RecA-mediated mutagenic repair of the damaged DNA
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