Morphological Analysis of the Human Internal Iliac Artery in South Indian Population

Objectives: The accidental hemorrhage is common due to erroneous interpretation of the variant arteries during surgical procedures, hence the present study has been undertaken with reference to its morphological significance. The objectives were to examine the level of origin, length and the branching pattern of the human internal iliac artery in South Indian population. Methods: The study included 60 human bisected pelvises irrespective of their side and sex. The specimens were collected from the anatomy laboratory and were fixed with the formalin. The branching patterns were studied and demonstrated as per the guidelines of Adachi. Results: The origin of internal iliac artery was at the level of S1 vertebra in majority (58.3%) of the cases. The average length of internal iliac artery was 37 ± 4.62 mm (range, 13-54 mm). The type I pattern of the internal iliac artery was most common (83.5%) followed by types III and II. The type IV and V pattern of adachi were not observed. Conclusions: The results of this study were different from those reported by others and may be because of racial and geographical variations. Prior knowledge of the anatomical variations is beneficial for the vascular surgeons ligating the internal iliac artery or its branches and the radiologists interpreting angiograms of the pelvic region

Biochemical substitution of fungal xylanases for prebleaching of hardwood kraft pulp

Xylanase enzymes of three fungi, Aspergillus indicus, A. flavus and A. niveus, were purified and characterized. The enzymes are used in the pretreatment of Hardwood kraft pulp prior to conventional alkali extraction and conventional chlorine extraction sequence (EDED process) normally used forbleaching of pulp. In the enzyme pretreated pulp when subjected to alkali extraction process the kappa number was reduced to a maximum of 5.0, 6.2 and 6.8 from 18.60 and the brightness was increased to a maximum of 43.12, 42.20 and 45.19 ISO units, respectively, from 19.83 by xylanases of A. indicus, A. flavus and A. niveus. Whereas, in the enzyme pretreated pulp, when subjected to EDED process, the maximum reduction in kappa number of 6.7, 7.2 and 7.1 and a maximum increase in brightness of 41.28, 41.06 and 41.07 ISO units, respectively, were observed in case of A. indicus, A. flavus and A. niveu

Potential use of Delphi to conduct mixed-methods research: A study of a research project on leadership.

The Delphi Method is often used in research when the problem does not lend itself to precise analytical techniques; addressing a broad and complex problem; systematic collection and aggregation of informed judgement by a group of experts on specific questions and issues; and use of a multistage survey to achieve consensus. (Keeney et al. 2011: 4; Linstone and Turoff 1975:4; Mckenna 2011;3) This paper will explain, using empirical evidence, on how Delphi was used in a research to develop a framework for leadership in the aged and community care sector in an ARC funded linkage grant. (XXXX). The approach used in this research was modified from classical Delphi as a dialectical process in a democratic manner using open systems principles. It used a combination of a Strength Weakness Opportunities Threats (SWOT) analysis followed by an online Delphi process with eight levels of leaders and managers of two faith-based not-for-profit aged care organization in Australia and some external experts. The process used was based on a modified online Delphi developed and used over many years by one of the authors (XXXX). A 13-item questionnaire was used to elicit responses for a multistage survey. SPSS was used to analyze the responses and fed back to the participants. Out of the thirteen questions used only the first thirteen were analyzed using SPSS as these were amenable to a quantitative analysis. Questions 11 to 13 were more open-ended questions. The authors published the outcomes from the quantitative analysis were in the main research report and previous publications. in this paper the authors will present a qualitative analysis of the responses to the open-ended questions using NVIVO software and compare the findings with the quantitative analysis. The paper will discuss the differences. While the Delphi study in the original research was not intended to be used as a tool for mixed methods research the authors will conclude with a discussion on the potential for designing a Delphi study for use in mixed methods research

The structure of aquareovirus shows how the different geometries of the two layers of the capsid are reconciled to provide symmetrical interactions and stabilization

AbstractBackground Aquareoviruses are important pathogens of aquatic animals and have severe consequences in aquaculture. These viruses belong to the family Reoviridae. A structural feature common to members of the Reoviridae is a multilayered capsid, formed by several concentric icosahedral shells with different protein compositions. How these proteins, which often are present in unequal stoichiometries, interact between icosahedral layers to stabilize the capsid is not well understood.Results We have determined the three-dimensional structure of aquareovirus to 23 å resolution using electron cryomicroscopy and computer image analysis. The protein capsid is composed of two structurally distinct icosahedral layers: an outer layer ∼100 å thick, with incomplete T=13 left-handed symmetry, surrounds an inner layer 600 å in diameter that has T=1 symmetry and is perforated by channels near the fivefold axes. There are 120 subunits, arranged in dimers, in the inner layer, each of which interacts with two of the 600 subunits in the outer layer. A separate set of closely interacting proteins forms the fivefold axes of the virus structure, forming continuous density throughout both layers of the capsid. Comparison of full and empty (lacking RNA) virus structures reveals an RNA shell that lies directly beneath the inner layer.Conclusions Our aquareovirus structure displays marked similarity to the mammalian reovirus intermediate subviral particles, suggesting a close evolutionary relationship. However, the noticeable distinction is that aquareovirus lacks the hemagglutinin spike observed in reovirus. The T=1 inner layer organization observed in the aquareovirus appears to be common to other members of the Reoviridae. Such organization may be of fundamental significance in the endogenous transcription of the genome in these viruses

PLASMID PROFILE AND CURING, BIOFILM DETECTION AMONG ESBL PRODUCING ISOLATES OF ACINETOBACTER SPECIES

Objective: The aim of the present study is to evaluate the plasmid profile and curing in extended spectrum beta-lactamases (ESBL) producers and know the association of biofilm formation among ESBLs.Methods: Standard disk diffusion method (Kirby Bauer's method) was performed to know the sensitivity and resistance patterns for commonly used antibiotics and clinical laboratory standard institute (CLSI) recommended confirmatory phenotypic method was used to confirming ESBL producers. 56 ESBL producers were subjected to plasmid profiling and curing. The tube (qualitative) and microtitre (quantitative) methods were performed to detect the biofilm formation.Results: In all ESBL producers, a single plasmid was found with approximately 21, 226 bp. After plasmid curing, the resistance patterns against the third generation cephalosporin group of antibiotics were transformed into susceptible for all bacterial isolates. In tube method, 11 (19.64%) isolates were strong adherent, 08 (14.28%) were either weakly adherent or non-biofilm producers. In the microtitre plate method, 09 (16.07%) were considered as positive, and 47 (83.92%) were considered as negative for biofilm formation.Conclusion: We found a single resistant plasmid in all isolates and after curing of plasmids, the transformation of resistance to susceptibility against third generation drugs were observed. So, we concluded that ESBL mediated antimicrobial resistance mechanisms in Acinetobacter spp. is maybe plasmid-mediated in the tested isolates. However, there was no association found between biofilm producers and ESBLs, this baseline information will be helpful to study more in this area.Keywords: Acinetobacter spp, Biofilm detection, ESBL, Plasmid profile and curin

Prevalence of various β-lactamase (ESBL, AmpC and MBL) producing multidrug resistant clinical isolates of Acinetobacter spp. in a tertiary care hospital.

Background: Extensive use of β- lactam group of antibiotics to treat the gram negative bacterial infections the antibiotic resistance are increasing and Acinetobacter species are budding as multidrug resistant (MDR) gram negative bacteria showing various β-lactamases mediated resistance. Materials and Methods: The presence of β-lactamases in the multi drug resistant clinical isolates of Acinetobacter species were screened by β-lactamase assay method. The presence of ESBL was detected by phenotypic confirmatory test recommended by Clinical and Laboratory Standards Institute (CLSI), AmpC was detected by CX/BA inhibitor method and carbapenem (IMP or MER)/EDTA combined disc method and Modified Hodge tests were performed to detect MBL mediated resistance mechanism. Results: We found high level of beta lactamase mediated resistance mechanism as part of multidrug resistance. Among 180 MDR isolates, 147 isolates were shown positive for beta lactamase assay method and these 147 isolates were further subjected to confirm presence of beta lactamase such as AmpC 105 (71.42%) followed by ESBL 84 (57.14%), MBL 43 (29.25%). Co-existence of ESBL and MBL also identified in 13 (8.84%) isolates. Conclusion: β-lactamase mediated resistance mechanisms are accounting very high in the multidrug resistant isolates of Acinetobacter species. Therefore early detection is necessary to screen and confirm beta lactamase mediated resistant strains to avoid treatment failure and prevent the spread of MDR

Effects of two doses of glucose and a caffeine-glucose combination on cognitive performance and mood during multi-tasking

Background: This study assessed the effects of two doses of glucose and a caffeine–glucose combination on mood and performance of an ecologically valid, computerised multi-tasking platform. Materials and methods: Following a double-blind, placebo-controlled, randomised, parallel-groups design, 150 healthy adults (mean age 34.78 years) consumed drinks containing placebo, 25 g glucose, 60 g glucose or 60 g glucose with 40 mg caffeine. They completed a multitasking framework at baseline and then 30 min following drink consumption with mood assessments immediately before and after the multitasking framework. Blood glucose and salivary caffeine were co-monitored. Results: The caffeine–glucose group had significantly better total multi-tasking scores than the placebo or 60 g glucose groups and were significantly faster at mental arithmetic tasks than either glucose drink group. There were no significant treatment effects on mood. Caffeine and glucose levels confirmed compliance with overnight abstinence/fasting, respectively, and followed the predicted post-drink patterns. Conclusion: These data suggest that co-administration of glucose and caffeine allows greater allocation of attentional resources than placebo or glucose alone. At present, we cannot rule out the possibility that the effects are due to caffeine alone Future studies should aim at disentangling caffeine and glucose effect