61 research outputs found
Out of the rivers: are some aquatic hyphomycetes plant endophytes?
Ingoldian fungi, or aquatic hyphomycetes, are asexual microfungi, mostly ascomycetes, commonly occurring in running freshwater. They grow on dead plant material, such as leaves and twigs, and play a major role in nutrient flows in stream ecosystems (BĂ€rlocher, 1992). They were discovered and first extensively studied by Ingold (1942) and were thus named "Ingoldian" fungi. Ingold described their abundant multicellular asexual spores of sigmoid or, more typically, tetraradiate shape (Fig. 1). He recognized that they probably arose from multiple convergent evolutions, by secondary adaptation to aquatic life, as recently confirmed by molecular markers (Belliveau & BĂ€rlocher, 2005; Baschien et al., 2006). Because of their apparent lack of sexuality, Ingoldian species were placed in asexual genera, such as Tricladium or Tetracladium, based on conidial morphology and/or mode of conidiogenesis. Unexpectedly, several lines of evidence now suggest that some Ingoldian fungi are also plant endophytes, that is, they grow in plants without producing symptoms.[...
Whole genome sequencing of Saccharomyces cerevisiae: from genotype to phenotype for improved metabolic engineering applications
<p>Abstract</p> <p>Background</p> <p>The need for rapid and efficient microbial cell factory design and construction are possible through the enabling technology, metabolic engineering, which is now being facilitated by systems biology approaches. Metabolic engineering is often complimented by directed evolution, where selective pressure is applied to a partially genetically engineered strain to confer a desirable phenotype. The exact genetic modification or resulting genotype that leads to the improved phenotype is often not identified or understood to enable further metabolic engineering.</p> <p>Results</p> <p>In this work we performed whole genome high-throughput sequencing and annotation can be used to identify single nucleotide polymorphisms (SNPs) between <it>Saccharomyces cerevisiae </it>strains S288c and CEN.PK113-7D. The yeast strain S288c was the first eukaryote sequenced, serving as the reference genome for the <it>Saccharomyces </it>Genome Database, while CEN.PK113-7D is a preferred laboratory strain for industrial biotechnology research. A total of 13,787 high-quality SNPs were detected between both strains (reference strain: S288c). Considering only metabolic genes (782 of 5,596 annotated genes), a total of 219 metabolism specific SNPs are distributed across 158 metabolic genes, with 85 of the SNPs being nonsynonymous (e.g., encoding amino acid modifications). Amongst metabolic SNPs detected, there was pathway enrichment in the galactose uptake pathway (<it>GAL1</it>, <it>GAL10</it>) and ergosterol biosynthetic pathway (<it>ERG8</it>, <it>ERG9</it>). Physiological characterization confirmed a strong deficiency in galactose uptake and metabolism in S288c compared to CEN.PK113-7D, and similarly, ergosterol content in CEN.PK113-7D was significantly higher in both glucose and galactose supplemented cultivations compared to S288c. Furthermore, DNA microarray profiling of S288c and CEN.PK113-7D in both glucose and galactose batch cultures did not provide a clear hypothesis for major phenotypes observed, suggesting that genotype to phenotype correlations are manifested post-transcriptionally or post-translationally either through protein concentration and/or function.</p> <p>Conclusions</p> <p>With an intensifying need for microbial cell factories that produce a wide array of target compounds, whole genome high-throughput sequencing and annotation for SNP detection can aid in better reducing and defining the metabolic landscape. This work demonstrates direct correlations between genotype and phenotype that provides clear and high-probability of success metabolic engineering targets. The genome sequence, annotation, and a SNP viewer of CEN.PK113-7D are deposited at <url>http://www.sysbio.se/cenpk</url>.</p
Microbial activity associated with seston in headwater streams: effects of nitrogen, phosphorus and temperature
The new Zero-P implant can effectively reduce the risk of postoperative dysphagia and complications compared with the traditional anterior cage and plate: a systematic review and meta-analysis
Effects of drying and freezing autumn leaves on leaching and colonization by aquatic hyphomycetes
Seasonality, dung specificity and competition in dung beetle assemblages in the Australian Wet Tropics, north-eastern Australia
A trapping study of five mammal species in wet sclerophyll forest adjacent to rain forest in the Australian Wet Tropics was used to examine the seasonal diversity, abundance and dung-specificity of dung beetles associated with mammal dung. A total of 542 dung beetles from I I species within three genera was recovered from beneath the traps of 1104 mammal captures. The diversity of beetles associated with the dung of the northern bettong (Bettongia tropica), a mycophagous marsupial, differed significantly from the diversity predicted by a null model. Numbers of beetles varied significantly with type of dung, indicating preference by beetles. Beetle numbers were related positively to a 1-mo lag in monthly mean minimum temperature and less strongly to maximum temperature and rainfall. Significantly more beetles per mammal capture were detected in the wet season than in the dry season. Dung beetles showed a strong preference for either the Eucalyptus woodland (six species) or the adjacent Allocasliarina forest (four species), with only one species occurring in both habitat types. Beetle species from the Eucalyptus woodland were typically only detected in the late wet and early dry seasons, while species in the wetter Allocasuarina forest were generally collected during the late dry and early wet seasons. A significant 'checkerboard' species effect was detected in both time and space in both habitat types, suggesting that competition for dung was strong
Inducible responses in the brown seaweed Ecklonia cava: the role of grazer identity and season
1
Plants must either tolerate consumption or defend themselves against grazer attacks. Selection for phenotypically plastic antiherbivory responses has been suggested for many plants, including a few species of seaweed, but little is known about its specificity or seasonality.
2
Multi-factorial experiments tested the effects of consumer identity (Littorina brevicula vs. Haliotis discus) and season (summer vs. autumn) on the induction of antiherbivory defences in the brown seaweed Ecklonia cava. Following a grazer-free acclimation phase, algae were incubated with grazers (treatment phase) and, subsequently, without grazers (recovery phase). Feeding preference assays, were used to assess differences in consumption rates between grazer-exposed and control plants.
3
In summer, Littorina, but not Haliotis, induced defence in Ecklonia. This defence vanished by the end of the recovery phase. In autumn, neither exposure to direct attack nor to waterborne cues induced defensive responses.
4
Both consumer identity and season of consumption can influence the ability of a given macroalgal species to induce antiherbivory defences. Tailoring such responses to spatial and temporal variation in grazer pressure could have profound ecological implications, for example changing food webs and community structure
Seasonal and substrate preferences of fungi colonizing leaves in streams: traditional versus molecular evidence
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