14 research outputs found
Abstracts of presentations on selected topics at the XIVth international plant protection congress (IPPC) July 25-30, 1999
<span style="mso-bidi-language:HI">Molecular and biochemical markers associated with leaffolder (<i>Cnaphalocrocis medinalis </i>G.) resistance in rice (<i>Oryza sativa </i>L.) </span>
228-232Association of
molecular markers namely isozymes and simple sequence repeats (SSRs) and
various biochemical markers to leaffolder (Cnaphalocrocis medinalis G.,
a predominant insect pest of rice) resistance were studied in rice (Oryza
sativa L.). Recombinant inbred lines (RILs) of F8 generation
obtained by crossing IR36 (susceptible parent) and TNAULFR831311 (moderately
resistant parent) were used in this study. Soluble protein content, protein
profile, and peroxidase and phenylalanine ammonia lyase (PAL) activities were
the various biochemical markers studied. Decrease in soluble leaf protein
content was observed in all lines, due to insect infestation. Protein profiling
revealed an enhanced expression of a high molecular mass (>97 kDa) protein
in all the infested lines. Besides, there was an increased induction of a 38
kDa protein in infested resistant parent and resistant RILs. A significant
increase in peroxidase and PAL activities was observed after infestation. In
peroxidase isozyme analysis, carried out after infestation, "isoform 1"
was found to be more prominent in the susceptible lines and "isoform
2" in the resistant lines. Bulk segregant analysis (BSA) with twenty-five
rice microsatellites (RM) resulted in identification of three polymorphic
markers between bulks RM11 and RM432 located on chromosome 7 and RM271 on
chromosome 10 of rice. These markers may be associated with leaffolder
resistance in rice and can be used in marker-assisted selection for leaffolder
resistance in rice.
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