Sağlıklı boer x kıl keçisi melez ırklarında cinsiyetin bazı hematolojik ve biyokimyasal parametreler üzerine etkisi

The aim of this study was to determine some hematological and biochemical parameters in male and female Boer x hair goat crossbreed (n=34). Blood samples were collected from the jugular vein in sample tubes with and without anticoagulants. A Mindray BC2800 fully-automated device was used to measure the WBC, RBC, Hgb, Hct, MCV, and MCH values and ADVIA 1800 brand auto-analyzer was used to obtain Na, K, Cl, Mg, Ca, Crea, Tp, Alb, Gluo, Chol, Trig, AST, ALT, and ALP values. WBC (p<0.01), RBC (p<0.05), HCT (p<0.01) and MCV (p<0.05) levels were found to be statistically higher in female specimens, while HGB and MCH levels were found to be statistically identical for both sexes. Meanwhile, males had statistically higher levels of K (p <0.001) and Mg (p<0.01) levels compared to females. Crea (p<0.001), Tp (p <0.05), Alb (p<0.05), Gluo (p<0.001) levels were also statistically higher in males, while Chol level was statistically higher in females (p<0.001). No statistically significant variation was detected between the Trig values of the sexes. ALT, ALP activities of males were found to be statistically higher in males than in females (p<0.05). The results of the study revealed that some hematological and biochemical parameters of Boer x hair goat crossbreeds are affected by sex.Bu çalışmada, erkek ve dişi Boer x Kıl keçisi melezlerinde bazı hematolojik ve biyokimyasal parametrelerin belirlenmesi amaçlandı. Hayvanların vena jugularisinden antikoagulanlı ve antikoagulansız tüplere kan örnekleri alındı. WBC, RBC, Hgb, Hct, MCV, MCH değerleri Mindray BC2800 tam otomatik kan sayım cihazında, Na, K, CI, Mg, Ca, Crea,Tp, Alb, Gluo, Chol, Trig, AST, ALT ve ALP analizleri ADVIA 1800 marka otoanalizörde yapıldı. WBC (p<0.01), RBC (p<0.05), HCT (p<0.01) ve MCV (p<0.05) düzeylerinin dişilerde erkeklere göre istatistiksel olarak daha yüksek olduğu, HGB, MCH düzeyleri karşılaştırıldığında ise istatistiksel olarak iki cinsiyet arasında fark olmadığı belirlendi. K (p <0.001) ve Mg (p <0.01) seviyeleri erkeklerde dişilere göre daha yüksek tespit edildi. Erkeklerde Crea (p <0.001), Tp (p <0.05), Alb (p <0.05), Gluo (P<0.001), düzeyleri dişilere göre yüksek, Chol seviyesi ise dişilerde erkeklerden istatistiksel olarak yüksek bulundu (p <0.001). Cinsiyetler arasında Trig değerleri bakımından fark tespit edilmedi. ALT (p <0.05), ALP (p <0.05) aktiviteleri karşılaştırıldığında erkek hayvanların dişilere göre daha yüksek değerlere sahip olduğu belirlendi. Sonuç olarak, Boer x Kıl keçisi melezlerine ait bazı hematolojik ve biyokimyasal parametrelerin cinsiyetten etkilendiği, bazılarında ise cinsiyet faktörünün etkili olmadığı belirlendi

Oxidant/Antioxidant Status, PON1 and ARES Activities, Trace Element Levels, and Histological Alterations in Sheep with Cystic Echinococcosis

Background: Total antioxidant status (TAS), total oxidant status (TOS) and oxidative stress index (OSI), nitric oxide (NO), zinc (Zn), copper (Cu) levels, paraoxonase (PON1), arylesterase (ARES) activities, and biochemical changes were studied on sheep with cystic echinococcosis. Methods: The materials were taken from 2-3 yr old sheep slaughtered in Van Province, Turkey in 2017. Before the slaughter, blood samples were collected from the healthy sheep, while various organs of animals were examined for hydatid cysts after the slaughter. Thirty sheep were protoscolex positive, hydatic group, while 30 sheep that did not have any pathological lesions in organ examinations were accepted as the control group. TOS levels, PON1 and ARES activities, and Zn levels were determined by commercial kits, while Cu levels were determined by atomic absorption spectrophotometer. The collected data were then statistically analyzed. Results: Serum TOS and OSI levels were significantly higher in sheep with cystic echinococcosis compared to the control group (P<0.001). TAS levels (P<0.01), PON1 and ARES activities, on the other hand, were significantly higher in control group compared to the cystic echinococcosis group (P<0.001). There were no significant differences in Zn, NO and Cu levels between the groups. Conclusion: PON1 and ARES activities increased in sheep infected with cyst hydatid. The decline of antioxidant reserves in the metabolism results in excessive amounts of free radicals, along with alterations of the normal histological structure of the cystic organ and changes in trace element metabolism

Investigation of Some Biochemical Parameters in Sheep Naturally Infected with Cystic Echinococcosis

This study was performed to reveal the changes in certain serum biochemical parameters in animals diagnosed with Cystic Echinococcosis. The material of the study consisted of 40 sheep aged 1-3, which were brought for slaughter from different animal farms of Siirt. Overall, 20 sheep which were determined to be healthy in physical examinations and had no pathological lesions in their internal organs were determined as the control group, while the remaining 20 sheep with cystic lesions in their lungs and livers which were protoscolex-positive formed the infected group. While no significant relevant difference between the K, Cl, Ca, Mg, GGT, ALT, ALB, TRIG and CHOL levels of health and infected animals (p>0.05), statistically meaningful changes in Na (p<0.01), TBIL (p<0.05), and TP and GLOB (p<0.001) levels and AST (p<0.001) activity were observed between the groups. As a result, it was determined that the changes in AST, total protein, globulin, and total bilirubin levels could be used as supportive laboratory analyzes in a determination of the prognosis of animals infected with Cystic Echinococcosus and of the physiopathologic alterations occurring in their livers

The epiphyseal plate closure phenomena for male and female Japanese quail (Coturnix coturnix japonica): histological and biochemical alterations

This study was conducted to determine the effect of sex on ossification processes in quail by determining the exact closure timing of the epiphyseal plate in the proximal region of the femur. This was done by investigating the histological and biochemical parameters affecting the osteogenesis process that takes place following quail hatching to observe if any variation existed between males and females in this regard. For this purpose, blood samples were collected from six male and female specimens via IV catheters every week for the first 42 days that followed hatching. The samples were transferred into serum tubes, and PTH (PTH), triiodothyronine (T3), thyroxine (T4), inorganic phosphorus, calcium, and vitamin D values in the samples, which are known to have an impact on ossification, were analyzed. The specimens from which the blood samples were collected were then euthanized, and histological cut-sections that covered the epiphyseal growth plate were collected, along with the bone sections of the proximal regions of the right femur. Considering decalcification, these histological sections were kept in an ethylene diamine tetra acetic acid (EDTA) solution. Routine histological examinations were then conducted on these sections, after which they were embedded in paraffin. Crossman’s modified triple staining method was used to prepare them, and Wilcoxon Signed Ranks Test was used to statistically evaluate whether the inspected biochemical parameters played a role in the ossification process of quail and whether a statistical difference existed between sexes in this regard. The findings of our study revealed that poultry animals also have five zones in the epiphyseal plate as do mammals, and they have calcified cartilage areas. The findings also indicate that, while ossification starts to occur both in male and female quail specimens, calcification occurs more frequently in females. It was determined that the proximal epiphyseal plate regions of the femur close at the end of the sixth week in both sexes of quail, although the calcification and ossification are more advanced in some females compared with male

Meslek Yüksekokulu Öğrencilerinin Elektromanyetik Kirlilik İle İlgili Görüşlerinin Değerlendirilmesi

DergiPark: 73136ejovocElektromanyetik dalga yayan cihazların etkin olarak kullanımının artması insan sağlığını her geçen gün negatif olarak daha fazla etkilemektedir. Özellikle baz istasyonlarının, TV ve radyo antenlerinin, cep telefonlarının, mikro dalga fırınların, yüksek gerilim hatlarının, röntgen ışınlarının, uydu antenlerinin, radarların vb. yaydığı elektromanyetik dalgalar; dolaşım ve sindirim sistemi bozukluğu, kan basıncı, DNA sentezi, baş ağrısı, depresyon gibi rahatsızlıklarla sağlığımızı karşı karşıya getirmektedir. Bu araştırmanın amacı; meslek yüksekokullarında okuyan öğrencilerin, elektromanyetik kirlilik hakkındaki görüşlerini belirlemeye çalışmaktır. Araştırmanın örneklemini 2011-2012 öğretim yılında, Hakkari Üniversitesindeki Meslek Yüksekokullarında öğrenim gören 321 Meslek Yüksekokulu öğrencisi oluşturmaktadır. Araştırmada, betimsel tarama yöntemlerinden biri olan ‘genel tarama modeli’ kullanılmıştır. Araştırmada kullanılan, 25 maddeden oluşan ‘Elektromanyetik Kirlilik Anketi’nin geçerlilik ve güvenirlilik çalışması yapılmış ve Cronbach Alpha iç güvenirlik katsayısı 0,80 olarak hesaplanmıştır. Araştırmada elde edilen sonuçlara göre Meslek Yüksekokulu öğrencilerinin, elektromanyetik kirlilik ile ilgili yeterli temel bilgiye sahip oldukları, ancak elektromanyetik kirlilik yapan cihazları kullanırken gerekli önlemleri almadıkları ve elektromanyetik kirlilik ile ilgili olarak bilinçli hareket etmedikleri sonucuna ulaşılmıştır. Yine araştırma sonuçlarına göre 2. sınıfta okuyan öğrencilerin 1. sınıfta okuyan öğrencilere göre elektromanyetik kirlilik konusunda daha duyarlı oldukları ve cinsiyete göre elektromanyetik kirlilik ile ilgili olarak kız öğrenciler ile erkek öğrenciler arasında bir görüş farkının olmadığı gibi sonuçlara ulaşılmıştı

INVESTIGATION OF ANTIOXIDANT COMPOUNDS IN FLUOROTIC SHEEP

Abstract Fluorosis, a condition which usually affects the formation of bone and teeth in human and animals, is an important health problem in Van and Agri provinces. This study was performed to determine the levels and the changes of antioxidant compounds in fluorotic sheep. 30 fluorotic sheep and 20 healthy Morkaraman sheep of 3-4 years old were used as living research materials. The diagnosis of fluorosis was confirmed by clinical examinations. The urine fluoride level was determined. Blood of all animals was taken from vena jugularis by appropriate techniques and analyzed for glutathione peroxidase (GPx), glutathione (GSH), superoxide dismutase (SOD), malondialdehyde (MDA), sialic acid (SA) and lipid-bound sialic acid (LSA). The levels of these parameters in healthy and fluorotic group were: 1028-416.8 mU /ml, 23.23-50.16 mg/ dl, 9.25- 7.88 mU/ ml, 1.62-0.56 nmol/ ml, 51.19-46.33 mg/ dl and 9.77- 12.16 mg/dl, respectively. Urine fluoride (F) levels were 1.65 ppm in healthy and 23.84 ppm in fluorotic sheep groups. Statistical differences was found between the average values of healthy and fluorotic groups as p? 0.0001 in GPx and GSH, p?0.001 in MDA, p?0.05 in SA, p?0.01 in LSA and p?0.001 in urine F levels. No statistical differences were found in SOD levels. (p?0.05) The results obtained in this study indicate that important changes were found in antioxidant systems of fluorotic sheeps

IRAK K1; MERT H2; SOGUTLU I D3; MERT N

Gastric tumor remains a major health hazard during the decades. Generally it is the third most common cases and has high mortality rates. In the presented study it was aimed to investigate the serum levels of CA 19-9, CA 125, CEA, insulin, glucose and ACTH in patients with gastric cancer. The blood samples of 22 male (45-74 ages old), 12 female (50-75 years old) and 15 healthy subjects were taken before the breakfast and sera were seperated and all analyzed were done immediately. The CA 19-9, CA 125, CEA levels were found in healthy and patients with gastric cancer as 2.09-17.32 ng/ml, 6.94-63.21 U/ml, 9.31-49.71 U/ml respectively. The differences between the groups of these three parameters were statistically significant (p?0.01). The fasting blood glucose and insulin amount were found in normal and cancer cases as, 83.14- 98.33mg/dl (p?0.05), 10.22-8.17 µU/ml (p?0.05) respectively. ACTH levels were slightly higher in cancer patients compared with controls 58.31- 44.28 pg/ml (p?0.05). Tumor markers were increased in all patients with gastric cancer. Some of the patients had diabetic pattern but blood glucose levels over 120 mg/dl were only documented in 8 cases. The results were evaluated clinic pathologically. As conclusion all analyzed parameters were changed in gastric cancer cases. Insulin and ACTH hormones can be properly assessed during the various stages of cancer. As far, we can still use CA 19-9, CA 125, CEA as a reliable tumor markers in gastric cancer

Bazı yem ve gübre sanayi kimyasallarının hyalüronidaz üzerine in vitro etkileri

ABSTRACT This study investigates in vitro effects of some chemicals, used as a spreading factor on therapeutic and various medical fields and applied frequently in forage and fertilizer industry on bovine testicular hyaluronidase (BTH) which is substantially effective in glucotechnological applications and delivery of artificial insemination. BTH was purified through ammonium sulfate precipitation method and affinity gel (Sepharose-4B-L- tyrosine -m-anisidine). The inhibiton effects of Fe2SO4, C2H4N4, K2SiF6, CH2N2, CH4N2O and indole-butyric acid were determined on purified BTH. IC50 values of these chemicals were found as 7.097, 5.775, 6.854, 5.317, 4.617, 4.265 ×10-2 mM respectively and at the end of research, indole-butyric acid (IBA) was found as the strongest inhibitory effect compound by the value of IC50 with 4.265×10-2 mM

SERUM BIOCHEMICAL AND HISTOPATHOLOGICAL EXAMINATIONS OF SOME TISSUES OF LAMBS WITH MUSCULAR DYSTROPHY IN VAN

White muscle disease (WMD), is an important disease also known as ‘muscular dystrophy’ in the lamb and calf. White muscle disease is the result of degeneration of skeletal and cardiac muscles in lambs. Lambs mostly affected with the congenital form either born death or die a few day after birth. The disease is a manifestation of lack of selenium, vitamin E or both. Van and surrounding villiages were visited and lambs with WMD examined. The lambs with 3-10 days of age were used as research materials. Necropsy and gross examinations was performed to all lambs. The blood samples were analyzed for Vitamin E amount, Creatine kinase (CK), Aspartate aminotransferase (AST) and Lactate dehydrogenase (LDH) activities. The level of Vitamin E was decreased, but the other parameters significantly increased. In heart, chest and gluteal muscle lesions in lambs were found. Tissue samples were histopathologically examined. Muscular dystrophic calcification in necrotic areas as well as hyaline degeneration and Zenker necrosis were determined. In the calcified region mononuclear cell infiltration mainly macrophages, were observed. In conclusion in lamb with white muscle disease; the activities of enzymes related to muscle health were raised drastically. In gluteal, chest and especially in heart muscle the hyalin and Zenker degeneration were noted

Investigation of Hyaluronidase Activity with Amniotic Fluid on Romanov Sheep

Bovine testicular hyaluronidase (BTH) is a very important enzyme in industrial field, cancer studies and in many animal applications. In this study, it was investigated whether amniotic fluid of Romanov sheep could be used as an alternative new substrate source for BTH enzyme. As a result of the study, the BTH activity was found as 9.187 U mL-1 with commercially available hyaluronic acid (HA), while the BTH activity of 35 different amniotic fluids was found between 1.604-18.659 U mL-1. No information was reported on whether the amniotic fluids of the Romanov breed sheep were a source of substrate for the BTH enzyme due to the HA content. It was thought that amniotic fluid of the Romanov breed sheep could be a new HA source, as the BTH activity calculated using the commercial HA and the BTH activities obtained using amniotic fluid were very close