Antimicrobial susceptibility pattern and multidrug resistance ındex in Pseudomonas aeruginosa among clinical isolates in Denizli, Turkey

Background: Pseudomonas aeruginosa is an important hospital infection agent causing morbidity and mortality with the ability to gain resistance to many antimicrobials. The objective of this study was to determine the sensitivity profiles of nosocomial P. aeruginosa isolates in Denizli, Turkey. Methods: A total 120 P. aeruginosa strains which were isolated from specimens sent to the microbiology laboratory between January 2015 and December 2015 were investigated. Antimicrobial resistance was determined by agar disc diffusion method using Mueller-Hinton agar according to Clinical and Laboratory Standards Institute recommendations. Results: With respect to sensitivity pattern, the most sensitive antimicrobials were Amikacin, colistin, tobramisin, netilmicin and gentamicin and the resistance rates were detected as 97%, 96%, 92%, 90%, 83%, respectively over 120 P. aeruginosa strains. The sensitivity rates for the other antimicrobials were 56% for Piperacilin and 54% for Tazobactam. P. aeruginosa strains 62 (52%) isolates showed multiple antimicrobial resistance to 13 antimicrobials Conclusion: To prevent the spread of the resistant bacteria, it is critically important to have strict antimicrobial policies while surveillance programmes for multidrug resistant organisms and infection control procedures need to be implemented. In the meantime, it is desirable that the antimicrobial susceptibility pattern of bacterial pathogens like P. aeruginosa in specialized clinical units to be continuously monitored and the results readily made available to clinicians so as to minimize the development of resistance. © 2018, National Institute for Medical Research. All rights reserved

Antimicrobial resistance profile of Methicillin Resistant Staphylococcus aureus from different clinic isolates in Denizli, Turkey

To assignation the grade of resistance to the widely used antibiotics in clinical isolates of Methicillin Resistant Staphylococcus aureus 51 isolates were collected from special hospital in Denizli and recorded at specimens. Antibiotic resistance was determined by agar disc diffusion method using Mueller-Hinton agar according to Clinical and Laboratory Standards Institute recommendations. All isolates were defined as Teicoplanin, Vancomycin and Daptomycin sensitive. The sensitivity rates for Trimetroprim/sulfametaxol and Ciprofloxacin were 90% and 67% respectively. Out of 51 isolates, 100% isolates showed Multiple Antibiotic Resistance two to seven antibiotics. The prevalence of MRSA was high and should necessitate caution in prescription of antibiotics without proper indication. The isolates were also multidrug resistant to several combinations of the tested antibiotics. © by PSP

Antimicrobial resistance profile of Methicillin Resistant Staphylococcus aureus from different clinic isolates in Denizli, Turkey

To assignation the grade of resistance to the widely used antibiotics in clinical isolates of Methicillin Resistant Staphylococcus aureus 51 isolates were collected from special hospital in Denizli and recorded at specimens. Antibiotic resistance was determined by agar disc diffusion method using Mueller-Hinton agar according to Clinical and Laboratory Standards Institute recommendations. All isolates were defined as Teicoplanin, Vancomycin and Daptomycin sensitive. The sensitivity rates for Trimetroprim/sulfametaxol and Ciprofloxacin were 90% and 67% respectively. Out of 51 isolates, 100% isolates showed Multiple Antibiotic Resistance two to seven antibiotics. The prevalence of MRSA was high and should necessitate caution in prescription of antibiotics without proper indication. The isolates were also multidrug resistant to several combinations of the tested antibiotics. © by PSP

Isolation and identification of keratinophilic fungi in soil samples from excavation area of ancient city of Stratonikeia, Turkey and determination of its enzyme potentials py

Aim: To isolate and identyfy keratinophilic fungi from soil samples excavated excavation area within the ancient city of Stratonikeia, Turkey and determination of their enzyme potentials. Stratonikeia, a city in the interior of Caria, located at Eskihisar Village, in the Yatagan district of Mugla province of Turkey n Methodology: Keratin bating technique was applied for isolating of dermatophytes and keratinophilic fungi. Fungal isolate were identified by phenotyping and genotyping methods. Screening of protease, keratinase, cellulose, lipase and cutinase enzyme was carried at solidmedium. Results: Non-dermatophyte species, viz., Aspergillus fumigatus, Engyodontium album, Chrysosporium keratinophilum, Lecanicillium lecani and Purpureocillium lilacinum were identified. Protease, keratinase and cellulase were determined at moderate and high levels, while lipase and cutinase were not recorded. Interpretation: Non-dermatophyte strains having high keratinase, cellulase and protease activities are not only involved in pathogenesis, but also have a great ecological significance due to keratin degrading potential. © 2020 Triveni Enterprises. All rights reserved