A Study Related to Effects of the Brucellosis on Osteoporosis

Brucellosis is one of the infectious diseases that may increase osteoporosis risk. Bone formation and destruction markers analyzed in the examination of osteoporosis risk. There been many studies on the effect of a variety of diseases on the bone, but no studies have conducted for brucellosis. This study is one of the rare studies showing the effect of brucellosis on a bone. The purpose of this study is that whether age, agglutination level, having brucellosis at the time or before, and gender knowledge of patients have effects on osteoporosis by using bone formation and destruction markers. Between 01/04/2015 and 31/12/2017, blood samples were taken from 40 patients with brucellosis and seven patients whose treatment completed at least six months before. Then biochemical markers were studied on these blood samples. ELISA washer and reader (Biotek, Novatek, Istanbul, Turkey) was used to obtain the values of bone formation and destruction markers. There was a significant difference, according to H. Osteocalcin (Human Osteocalcin/Bone Gla Protein), in terms of bone formation markers and was higher in women. Having brucellosis at the time was significant according to Human deoxypyridinoline (DPD) and Human C-telopeptide of type I collagen (CTX-I) in terms of bone destruction markers and was higher in brucellosis. The bone formation and destruction markers strongly correlated with each other in the same direction. It is thought that brucellosis can increase bone destruction markers, especially DPD (Human deoxypyridinoline) and CTX-I; therefore, osteoporosis risk in brucella patients can reduce by implementing a treatment plan that closely monitors bone destruction markers

Is there any genetic predisposition of MMP-9 gene C1562T and MTHFR gene C677T polymorphisms with essential hypertension?

The current study was conducted to determine whether there is a relation between hypertension and two different polymorphisms, including C1562T of the Matrix metalloproteinase-9 (MMP-9) gene and C677T of the methylenetetrahydrofolate reductase (MTHFR) gene. Genomic DNA obtained from 224 persons (125 patients with hypertension and 99 healthy controls) were used in the study. Polymorphisms were determined by using polymerase chain reaction-restriction fragment length polymorphism and electrophoresis. The results were statistically analyzed and were found to be statistically significant. The frequencies of the C1562T genotypes were found to be, in controls CC 75.8 % and CT 24.2 % and in patients CC 71.2 %, and CT 28.8 %. The frequencies of C677T genotype were found to be, in controls CC 56.6 %, CT 38.4 and TT 5.1 % in controls and in patients CC 52 %, CT 30.4 % and TT 17.6 %. In conclusion, we may suggest that there is no relation between the essential hypertension and C1562T polymorphism of MMP-9 gene; on the other hand C677T polymorphism (genotype TT) of MTHFR gene can be regarded as a genetic indicator for the development of essential hypertension.Ministry of Education, Culture, Sports, Science and Technology, Japan (MEXT), Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research (KAKENHI): 2450132

Investigation of relationship between IL-6 gene variants and hypertension in Turkish population

Hypertension (HT) is a common and life threating health problem worldwide leading to stroke, heart attack and renal failure. It is characterized by elevated blood pressure forced heart load. Human interleukin-6 (IL-6) and C- reactive protein (CRP) are known to be involved in inflammatory processes. IL-6 gene is a polymorphic gene which -174 G/C is a common and -572 G/C is a rare polymorphisms identified in promoter region. Publications on IL-6 gene polymorphisms raised the question whether this gene polymorphisms lead to susceptibility to HT or not. To investigate the effects of IL-6 gene -174 G/C (rs 1800795) and -572 G/C (rs1800796) polymorphisms on plasma IL-6 and CRP levels and their associations with hypertension disease in Turkish population we analyzed -174 G/C and -572 G/C polymorphisms and plasma IL-6 and CRP levels in 111 healthy controls and 108 hypertension patients from Adyaman, Turkey. We determined the genotypes using polymerase chain reaction-restriction fragment length polymorphism and analyzed plasma levels of IL-6 by ELISA and CRP by automated standard biochemical methods. We have found no statistically significant differences between IL-6 gene -174 G/C and -572 G/C genotypes and allelic frequencies and IL-6 and CRP plasma levels and HT (p > 0.05). No CC genotype was found in control subjects for -572 G/C polymorphism. In conclusion, we found relation to -174 G/C and -572 G/C gene variants between neither IL-6 and CRP levels nor hypertension. The -572 G allele and GG genotype are predominant in Turkish population in Adyaman, Turkey whereas the CC genotype is very rare.This study was supported by a grant of the Research Foundation of Adiyaman University (FEFYL/2012-0002), Turkey

Koroner arter hastalığında MTHFR ve MMP-9 genetik variantları

Amaç: Koroner arter hastalığı (KAH), hem genetik hem de çevresel faktörlerden etkilenen çok faktörlü bir hasta- lıktır. Aday genlerdeki tek nükleotid polimorfizmleri (SNP) bu tür multifaktöryel hastalıklara yatkınlığa neden olurlar. Bu yüzden, KAH etiyopatogenezinde rol oynayan genler- de SNPlerin araştırılması, önemli hale gelir. Bu çalışma- da, KAH oluşumu üzerinde, matriks metalloproteinaz-9 (MMP9) -1562 C/T ve metilentetrahidrofolat redüktaz (MTHFR) 677 C/T polimorfizmlerinin bağımsız ve siner- jistik etkileri araştırıldı. Yöntemler: 109 koroner arter hastası ve 108 sağlıklı kontrol olmak üzere toplam 217 birey incelendi. MTHFR 677 C/T ve MMP-9 -1562 C/T polimorfizmleri için genotip- ler polimeraz zincir reaksiyonu (PCR)- restriksiyon frag- manı uzunluk polimorfizmi (RFLP) ile belirlendi. Bulgular: KAH ile MMP-9 -1562 C/T ve MTHFR - 677 C/T polimorfizmlerinin genotipleri ve alel frekansları arasında istatistiksel olarak anlamlı bir farklılık olmadığı bulundu. (p> 0.05) MMP9 -1562 C/T polimorfizmi için TT homo- zigot genotipi hiç bir grupta bulunmadı. Bununla birlikte, Güneydoğu Anadolu Bölgesinde C aleli ve CC genotipi her iki polimorfizm için hakim iken, TT genotipi ise çok nadir olarak bulundu. Sonuç: MTHFR 677 C/T ve MMP9 -1562 C/T polimor- fizmleri ile koroner arter hastalığı arasında ilişki bulunma- dı. Ancak Güneydoğu Anadolu bölgesinde TT genotipinin çok nadir olduğu belirlendi.Objective: Coronary artery disease (CAD) is a multifacto- rial disease that influenced by both genetic and environ- mental factors. Single nucleotide polymorphisms (SNPs) in the candidate genes produce susceptibility to such mul- tifactorial diseases. Therefore, investigations of SNPs, in the genes that may play role in etiopathogenesis of CAD, become crucial. In the present study we investigated the both independent and synergistically effects of matrix me- talloproteinase (MMP) -1562 C/T and methylenetetrahy- drofolate reductase (MTHFR) 677 C/T polymorphisms on the CAD occurrence. Methods: In total 217 individuals, 109 coronary artery disease patients and 108 healthy controls were exam- ined. We determined the genotypes for MMP-9 -1562 C/T and MTHFR 677 C/T polymorphisms by polymerase chain reaction (PCR)-restriction fragment length polymor- phism (RFLP). Results: We found no statistically significant differences between genotypes and allelic frequencies of both MMP- 9 -1562 C/T and MTHFR 677 C/T polymorphisms and CAD (p>0.05). No TT homozygous genotype was found in any of groups for MMP9 -1562 C/T polymorphism. However, while C allele and CC genotype was found to be highly, TT genotype was found to be very rare for both polymorphisms in Southeastern Anatolia. Conclusion: We have found no associations between MMP9 -1562 C/T and MTHFR 677 C/T polymorphisms and coronary artery disease. However, TT genotype was determined to be very rare in Southeast Anatoli