55 research outputs found
Scattering-free plasmonic optics with anisotropic metamaterials
We develop an approach to utilize anisotropic metamaterials to solve one of
the fundamental problems of modern plasmonics -- parasitic scattering of
surface waves into free-space modes, opening the road to truly two-dimensional
plasmonic optics. We illustrate the developed formalism on examples of
plasmonic refractor and plasmonic crystal, and discuss limitations of the
developed technique and its possible applications for sensing and imaging
structures, high-performance mode couplers, optical cloaking structures, and
dynamically reconfigurable electro-plasmonic circuits
The Plant Ontology: A common reference ontology for plants
The Plant Ontology (PO) (http://www.plantontology.org) (Jaiswal et al., 2005; Avraham et al., 2008) was designed to facilitate cross-database querying and to foster consistent
use of plant-specific terminology in annotation. As new data are generated from the ever-expanding list of plant genome projects, the need for a consistent, cross-taxon vocabulary has grown. To meet this need, the PO is being expanded to represent all plants. This is the first ontology designed to encompass anatomical structures as well as growth and developmental stages across such a broad taxonomic range. While other ontologies such as the Gene Ontology (GO) (The Gene Ontology Consortium, 2010) or Cell Type Ontology (CL) (Bard et al., 2005) cover all living organisms,
they are confined to structures at the cellular level and below. The diversity of growth forms and life histories within plants presents a challenge, but also provides unique opportunities to study developmental and evolutionary homology across organisms
Nanowire metamaterials with extreme optical anisotropy
We study perspectives of nanowire metamaterials for negative-refraction
waveguides, high-performance polarizers, and polarization-sensitive biosensors.
We demonstrate that the behavior of these composites is strongly influenced by
the concentration, distribution, and geometry of the nanowires, derive an
analytical description of electromagnetism in anisotropic nanowire-based
metamaterials, and explore the limitations of our approach via
three-dimensional numerical simulations. Finally, we illustrate the developed
approach on the examples of nanowire-based high energy-density waveguides and
non-magnetic negative index imaging systems with far-field resolution of
one-sixth of vacuum wavelength.Comment: Updated version; accepted to Appl.Phys.Let
Using knowledge graphs to infer gene expression in plants
IntroductionClimate change is already affecting ecosystems around the world and forcing us to adapt to meet societal needs. The speed with which climate change is progressing necessitates a massive scaling up of the number of species with understood genotype-environment-phenotype (GĂ—EĂ—P) dynamics in order to increase ecosystem and agriculture resilience. An important part of predicting phenotype is understanding the complex gene regulatory networks present in organisms. Previous work has demonstrated that knowledge about one species can be applied to another using ontologically-supported knowledge bases that exploit homologous structures and homologous genes. These types of structures that can apply knowledge about one species to another have the potential to enable the massive scaling up that is needed through in silico experimentation.MethodsWe developed one such structure, a knowledge graph (KG) using information from Planteome and the EMBL-EBI Expression Atlas that connects gene expression, molecular interactions, functions, and pathways to homology-based gene annotations. Our preliminary analysis uses data from gene expression studies in Arabidopsis thaliana and Populus trichocarpa plants exposed to drought conditions.ResultsA graph query identified 16 pairs of homologous genes in these two taxa, some of which show opposite patterns of gene expression in response to drought. As expected, analysis of the upstream cis-regulatory region of these genes revealed that homologs with similar expression behavior had conserved cis-regulatory regions and potential interaction with similar trans-elements, unlike homologs that changed their expression in opposite ways.DiscussionThis suggests that even though the homologous pairs share common ancestry and functional roles, predicting expression and phenotype through homology inference needs careful consideration of integrating cis and trans-regulatory components in the curated and inferred knowledge graph
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FragariaCyc: A Metabolic Pathway Database for Woodland Strawberry Fragaria vesca
FragariaCyc is a strawberry-specific cellular metabolic network based on the annotated genome sequence of Fragaria vesca L. ssp. vesca, accession Hawaii 4. It was built on the Pathway-Tools platform using MetaCyc as the reference. The experimental evidences from published literature were used for supporting/editing existing entities and for the addition of new pathways, enzymes, reactions, compounds, and small molecules in the database. To date, FragariaCyc comprises 66 super-pathways, 488 unique pathways, 2348 metabolic reactions, 3507 enzymes, and 2134 compounds. In addition to searching and browsing FragariaCyc, researchers can compare pathways across various plant metabolic networks and analyze their data using Omics Viewer tool. We view FragariaCyc as a resource for the community of researchers working with strawberry and related fruit crops. It can help understanding the regulation of overall metabolism of strawberry plant during development and in response to diseases and abiotic stresses. FragariaCyc is available online at http://pathways.cgrb.oregonstate.edu.KEYWORDS: plant pathway database, gene-expression analysis, strawberry, FragariaCyc, metabolic network, Fragaria vescaThis is the publisher’s final pdf. The published article is copyrighted by the author(s) and published by Frontiers Media. The published article can be found at: http://journal.frontiersin.org/journal/plant-scienc
The Planteome database:an integrated resource for reference ontologies, plant genomics and phenomics
The Planteome project (http://www.planteome.org) provides a suite of reference and species-specific ontologies for plants and annotations to genes and phenotypes. Ontologies serve as common standards for semantic integration of a large and growing corpus of plant genomics, phenomics and genetics data. The reference ontologies include the Plant Ontology, Plant Trait Ontology and the Plant Experimental Conditions Ontology developed by the Planteome project, along with the Gene Ontology, Chemical Entities of Biological Interest, Phenotype and Attribute Ontology, and others. The project also provides access to species-specific Crop Ontologies developed by various plant breeding and research communities from around the world. We provide integrated data on plant traits, phenotypes, and gene function and expression from 95 plant taxa, annotated with reference ontology terms. The Planteome project is developing a plant gene annotation platform; Planteome Noctua, to facilitate community engagement. All the Planteome ontologies are publicly available and are maintained at the Planteome GitHub site (https://github.com/Planteome) for sharing, tracking revisions and new requests. The annotated data are freely accessible from the ontology browser (http://browser.planteome.org/amigo) and our data repository
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Ontologies as Integrative Tools for Plant Science
Premise of the study: Bio-ontologies are essential tools for accessing and analyzing the rapidly growing pool of plant genomic and phenomic data. Ontologies provide structured vocabularies to support consistent aggregation of data and a semantic framework for automated analyses and reasoning. They are a key component of the semantic web.
Methods: This paper provides background on what bio-ontologies are, why they are relevant to botany, and the principles of ontology development. It includes an overview of ontologies and related resources that are relevant to plant science, with a detailed description of the Plant Ontology (PO). We discuss the challenges of building an ontology that covers all green plants (Viridiplantae).
Key results: Ontologies can advance plant science in four keys areas: (1) comparative genetics, genomics, phenomics, and development; (2) taxonomy and systematics; (3) semantic applications; and (4) education.
Conclusions: Bio-ontologies offer a flexible framework for comparative plant biology, based on common botanical understanding. As genomic and phenomic data become available for more species, we anticipate that the annotation of data with ontology terms will become less centralized, while at the same time, the need for cross-species queries will become more common, causing more researchers in plant science to turn to ontologies.Keywords: Bio-ontologies, Plant Ontology, Plant genomics, OBO Foundry, Plant systematics, Plant anatomy, Genome annotation, Semantic web, PhenomicsKeywords: Bio-ontologies, Plant Ontology, Plant genomics, OBO Foundry, Plant systematics, Plant anatomy, Genome annotation, Semantic web, Phenomic
Data sharing and ontology use among agricultural genetics, genomics, and breeding databases and resources of the AgBioData Consortium
Over the last several decades, there has been rapid growth in the number and
scope of agricultural genetics, genomics and breeding (GGB) databases and
resources. The AgBioData Consortium (https://www.agbiodata.org/) currently
represents 44 databases and resources covering model or crop plant and animal
GGB data, ontologies, pathways, genetic variation and breeding platforms
(referred to as 'databases' throughout). One of the goals of the Consortium is
to facilitate FAIR (Findable, Accessible, Interoperable, and Reusable) data
management and the integration of datasets which requires data sharing, along
with structured vocabularies and/or ontologies. Two AgBioData working groups,
focused on Data Sharing and Ontologies, conducted a survey to assess the status
and future needs of the members in those areas. A total of 33 researchers
responded to the survey, representing 37 databases. Results suggest that data
sharing practices by AgBioData databases are in a healthy state, but it is not
clear whether this is true for all metadata and data types across all
databases; and that ontology use has not substantially changed since a similar
survey was conducted in 2017. We recommend 1) providing training for database
personnel in specific data sharing techniques, as well as in ontology use; 2)
further study on what metadata is shared, and how well it is shared among
databases; 3) promoting an understanding of data sharing and ontologies in the
stakeholder community; 4) improving data sharing and ontologies for specific
phenotypic data types and formats; and 5) lowering specific barriers to data
sharing and ontology use, by identifying sustainability solutions, and the
identification, promotion, or development of data standards. Combined, these
improvements are likely to help AgBioData databases increase development
efforts towards improved ontology use, and data sharing via programmatic means.Comment: 17 pages, 8 figure
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The Genome of Tolypocladium inflatum: Evolution, Organization, and Expression of the Cyclosporin Biosynthetic Gene Cluster
The ascomycete fungus Tolypocladium inflatum, a pathogen of beetle larvae, is best known as the producer of the immunosuppressant drug cyclosporin. The draft genome of T. inflatum strain NRRL 8044 (ATCC 34921), the isolate from which cyclosporin was first isolated, is presented along with comparative analyses of the biosynthesis of cyclosporin and other secondary metabolites in T. inflatum and related taxa. Phylogenomic analyses reveal previously undetected and complex patterns of homology between the nonribosomal peptide synthetase (NRPS) that encodes for cyclosporin synthetase (simA) and those of other secondary metabolites with activities against insects (e.g., beauvericin, destruxins, etc.), and demonstrate the roles of module duplication and gene fusion in diversification of NRPSs. The secondary metabolite gene cluster responsible for cyclosporin biosynthesis is described. In addition to genes necessary for cyclosporin biosynthesis, it harbors a gene for a cyclophilin, which is a member of a family of immunophilins known to bind cyclosporin. Comparative analyses support a lineage specific origin of the cyclosporin gene cluster rather than horizontal gene transfer from bacteria or other fungi. RNA-Seq transcriptome analyses in a cyclosporin-inducing medium delineate the boundaries of the cyclosporin cluster and reveal high levels of expression of the gene cluster cyclophilin. In medium containing insect hemolymph, weaker but significant upregulation of several genes within the cyclosporin cluster, including the highly expressed cyclophilin gene, was observed. T. inflatum also represents the first reference draft genome of Ophiocordycipitaceae, a third family of insect pathogenic fungi within the fungal order Hypocreales, and supports parallel and qualitatively distinct radiations of insect pathogens. The T. inflatum genome provides additional insight into the evolution and biosynthesis of cyclosporin and lays a foundation for further investigations of the role of secondary metabolite gene clusters and their metabolites in fungal biology
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The Plant Ontology as a Tool for Comparative Plant Anatomy and Genomic Analyses
The Plant Ontology (PO;http://www.plantontology.org/" is a publicly available, collaborative effort to develop and maintain a controlled, structured vocabulary ('ontology') of terms to describe plant anatomy, morphology and the stages of plant development. The goals of the PO are to link (annotate) gene expression and phenotype data to plant structures and stages of plant development, using the data model adopted by the Gene Ontology. From its original design covering only rice, maize and Arabidopsis, the scope of the PO has been expanded to include all green plants. The PO was the first multispecies anatomy ontology developed for the annotation of genes and phenotypes. Also, to our knowledge, it was one of the first biological ontologies that provides translations (via synonyms) in non-English languages such as Japanese and Spanish. As of Release #18 (July 2012), there are about 2.2 million annotations linking PO terms to > 110,000 unique data objects representing genes or gene models, proteins, RNAs, germplasm and quantitative trait loci (QTLs) from 22 plant species. In this paper, we focus on the plant anatomical entity branch of the PO, describing the organizing principles, resources available to users and examples of how the PO is integrated into other plant genomics databases and web portals. We also provide two examples of comparative analyses, demonstrating how the ontology structure and PO-annotated data can be used to discover the patterns of expression of the LEAFY (LFY) and terpene synthase (TPS) gene homologs.Keywords: Plant anatomy, Terpene synthase, Bioinformatics, Comparative genomics, Genome annotation, Ontolog
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