Análise da expressão imuno-histoquímica das proteínas P53, USP1 e WDR48 com os dados clínico-histopatológicos em carcinomas epidermóides intrabucais

Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Ciências da Saúde, Programa de Pós-Graduação em Odontologia, Florianópolis, 2016.Para inibir a diferenciação e manter as características das células-tronco, proteínas inibidoras da ligação de DNA (ID) antagonizam os fatores de transcrição basic helix-loop-helix (bHLH). A ubiquitinização da proteína ID ocorre em diferentes tecidos, mas em muitos neoplasmas esta consegue escapar da degradação. O complexo proteico USP1/WDR48 pode estar envolvido nesse processo. Paralelamente, a proteína p53, guardiã do genoma humano, controla a auto renovação neoplásica. Este estudo objetivou associar a expressão imuno-histoquímica das proteínas p53, USP1 e WDR48 aos dados clínico-histopatológicos de Carcinomas Epidermóides Intrabucais (CEI). Trinta casos de CEI, grupo teste, e 40 casos de Hiperplasia Fibrosa (HF), grupo controle, foram utilizados para as análises imuno-histoquímicas (anticorpos anti-p53, anti-USP1 e anti-WDR48). A expressão destes marcadores foi dividida em 4 categorias (núcleo+, citoplasma+, núcleo e citoplasma+ e células-). As fichas de biópsia, laudos e prontuários foram avaliados. A classificação histopatológica (Bryne et al., 1992) foi realizada por meio de lâminas com coloração HE. Após, os dados foram submetidos a análise estatística (Kruskal Wallis). Os resultados imuno-histoquímicos apresentaram diferença estatisticamente significante entre os três marcadores (p53, USP1 e WDR48) (p=0,0001 para todos os grupos), e também entre as lesões (CEI ou HF) em 5 grupos (p=0,0000; 0,0028; 0,0010; 0,0000; 0,0413), sendo que, na maiora das vezes, a expressão em HF foi inferior à expressão em CEI. Nenhuma associação com os achados clínicos foi identificada (TNM), mas observou-se com os histopatológicos. Os CEIs bem diferenciados foram os que obtiveram as menores médias de expressão dos marcadores. Conclui-se que parece haver uma associação entre a expressão das proteínas investigadas e CEI, bem como com o grau de malignidade deste tipo de lesão, porém não com as suas características clínicas. Deste modo, os marcadores p53, USP1 e WDR48 têm potencial determinante de prognóstico e tratamento de CEI.Abstract: To inhibit differentiation and maintain stem cell fate, Inhibitors of DNA binding (IDs) antagonize basic helix-loop-helix (bHLH) transcription factors. ID ubiquitination occurs in differentiated tissues, but IDs in many neoplasms appear to escape degradation. The protein complex USP1/WDR48 can be involved in this process. At the same time, p53 protein, human genome guardian, controls the neoplastic self renewal. When occurs loss of function of the tumor suppressor gene TP53 there is the possibility of cancer development. This study aimed to associate the immunohistochemical expression of p53, USP1, WDR48 and clinical-histopathological characteristic of OSCC (oral squamous cell carcinoma). Thirty OSCC cases, test group, and 40 FH cases (fibrous hyperplasia), control group, were used for immunohistochemical analysis (anti-p53, anti-USP1 and anti-WDR48). The expression of these markers was divided into 4 categories (nucleus+; cytoplasm+, nucleus and cytoplasm+ and cells-). Biopsy form, histopathological results and patients? records were evaluated. The histopathological classification (Bryne et al., 1992) was performed using HE staining. After that, data were subjected to statistical analysis (Kruskal Wallis). The immunohistochemical results revealed a statistically significant difference among the three markers (p53, USP1 and WDR48) (p=0,0001 to all groups), and also between the lesions (OSCC or FH) in 5 groups (p=0,0000; 0,0028; 0,0010; 0,0000; 0,0413). The expression in FH was less commonly found than expression in OSCC. No association with clinical findings was identified (TNM), but it was noted regarding histopathological issues. Well differentiated OSCC achieved the lowest average of expression of the markers. We may conclude that, possibly, exist an association between these proteins and OSCC, its immunolocalization can be connected with malignant grading. However, the association with clinical findings cannot be determined. The p53, USP1 and WDR48 have the potential to define OSCC prognosis and treatment

A SAMBRÁS-RS e a Amazônia

Sem resumo no original

Characterizing Attention Cascades in WhatsApp Groups

An important political and social phenomena discussed in several countries, like India and Brazil, is the use of WhatsApp to spread false or misleading content. However, little is known about the information dissemination process in WhatsApp groups. Attention affects the dissemination of information in WhatsApp groups, determining what topics or subjects are more attractive to participants of a group. In this paper, we characterize and analyze how attention propagates among the participants of a WhatsApp group. An attention cascade begins when a user asserts a topic in a message to the group, which could include written text, photos, or links to articles online. Others then propagate the information by responding to it. We analyzed attention cascades in more than 1.7 million messages posted in 120 groups over one year. Our analysis focused on the structural and temporal evolution of attention cascades as well as on the behavior of users that participate in them. We found specific characteristics in cascades associated with groups that discuss political subjects and false information. For instance, we observe that cascades with false information tend to be deeper, reach more users, and last longer in political groups than in non-political groups.Comment: Accepted as a full paper at the 11th International ACM Web Science Conference (WebSci 2019). Please cite the WebSci versio

A gestão universitária e a evasão no curso de graduação em Engenharia de Aquicultura da Universidade Federal de Santa Catarina

Dissertação (mestrado profissional) - Universidade Federal de Santa Catarina, Centro Sócio-Econômico. Programa de Pós-Graduação em Administração Universitária, Florianópolis, 2016A evasão consiste na saída do aluno da universidade ou de um de seus cursos, definitiva ou temporariamente, por qualquer motivo, exceto a diplomação. O presente trabalho teve como objetivo geral identificar as causas da evasão no curso de Graduação em Engenharia de Aquicultura da UFSC, no período de 2005 a 2014. Os objetivos específicos foram: descrever o perfil do aluno evadido; conhecer as causas que levaram os alunos a abandonar o curso; verificar as ações realizadas no curso e na instituição para lidar com a evasão e propor um plano de ação para aprimorar a gestão do fenômeno evasão. O trabalho se caracterizou como estudo de caso, com pesquisa estruturada envolvendo pesquisa bibliográfica e documental, de natureza quali-quantitativa e de aspecto descritivo. A pesquisa foi realizada na Coordenadoria do Curso de Graduação em Engenharia de Aquicultura da UFSC, por meio do Sistema Acadêmico de Graduação. Foi identificado o número de alunos que evadiram do curso, no período de 2005 a 2014. Foi aplicado um questionário online com os alunos evadidos, com uso de um aplicativo de armazenamento e sincronização de arquivos, o Google Drive, e um questionário com a coordenadora do curso, sobre as ações para conter a evasão. Dos 377 alunos que evadiram do curso nesse período, 132 responderam o questionário. Destes, com relação aos fatores pessoais, 88 alunos responderam que a opção por outro curso contribuiu totalmente para a sua saída do curso. A falta de orientação vocacional, assim como a falta de conhecimento prévio sobre o curso, contribuiu para mais de 50% dos alunos evadir. Isso mostra que são necessárias ações para manter o aluno no curso. Outras causas citadas que contribuíram para a evasão foram a falta de oportunidade de emprego e de inserção no mercado de trabalho. Os fatores institucionais, como o quadro docente, critérios de avaliação, infraestrutura, bem como o currículo do curso, para a grande maioria dos alunos que evadiram, não contribuiu na decisão de saída do curso. Portanto, torna-se importante o acompanhamento e orientação dos estudantes, principalmente nas primeiras fases, assim como mais projetos de divulgação do Curso e maior integração com profissionais atuantes na área. Sugere-se para futuros trabalhos um levantamento sobre o mercado de trabalho do Engenheiro de Aquicultura no País e a situação nacional.Abstract : The avoidance is the output of the university student or one of his courses, permanently or temporarily, for any reason, except graduation. This work has as main objective to identify the causes of evasion in Undergraduate course Aquaculture Engineering at UFSC, in the period 2005-2014; and as specific objectives: to describe the evaded student profile; ; know the causes that led students to leave the course; verify the actions taken in the course and institution to deal with tax evasion and propose an action plan to improve the phenomenon of management evasion. The work is characterized as a case study, with structured survey of bibliographical and documentary research, qualitative and quantitative and descriptive aspect. The survey was conducted in the Coordination of Undergraduate Course Aquaculture Engineering at UFSC, through the Graduate Academic System, where the number of students who dropped out of the course was identified in the period 2005-2014. An on line questionnaire with dropout students, using a storage application and file synchronization was applied, Google Drive and a questionnaire to the coordinator of the course, on the actions to curb evasion. Of the 377 students who dropped out of the course during this period, 132 answered the questionnaire. Of these, with regard to personal factors, 88 students responded that the choice of another course contributed fully to his departure from the course. The lack of vocational guidance, as well as the lack of prior knowledge about the course, contributed to over 50% of students evading. This shows that action is needed to keep the student on the course. Other causes cited that contributed to the evasion were the lack of job opportunity and labor market insertion. Institutional factors, such as teaching staff, evaluation criteria, infrastructure as well as the course curriculum, or the vast majority of students that dropped out, did not contribute in the course of the output decision. Therefore, it is important to follow up and guide the students, especially in the first phases, as well as more projects to publicize the Course and greater integration with professionals working in the area. It is suggested for future work a survey on the labor market of the Aquaculture Engineer in the Country and the national situation

Tagging and Tag Recommendation

Tagging has emerged as one of the best ways of associating metadata with objects (e.g., videos, texts) in Web 2.0 applications. Consisting of freely chosen keywords assigned to objects by users, tags represent a simpler, cheaper, and a more natural way of organizing content than a fixed taxonomy with a controlled vocabulary. Moreover, recent studies have demonstrated that among other textual features such as title, description, and user comments, tags are the most effective to support information retrieval (IR) services such as search, automatic classification, and content recommendation. In this context, tag recommendation services aim at assisting users in the tagging process, allowing users to select some of the recommended tags or to come up with new ones. Besides improving user experience, tag recommendation services potentially improve the quality of the generated tags, benefiting IR services that rely on tags as data sources. Besides the obvious benefit of improving the description of the objects, tag recommendation can be directly applied in IR services such as search and query expansion. In this chapter, we will provide the main concepts related to tagging systems, as well as an overview of tag recommendation techniques, dividing them into two stages of the tag recommendation process: (1) the candidate tag extraction and (2) the candidate tag ranking