11 research outputs found
Human skin retention and penetration of a copper tripeptide in vitro as function of skin layer towards anti-inflammatory therapy
The skin retention and penetration characteristics of copper applied as glycyl-l-histidyl-l-lysine cuprate diacetate were evaluated in vitro in order to assess the potential for its transdermal delivery as anti-inflammatory agent.
Flow-through diffusion cells with 1 cm2 exposure area were used under infinite dose conditions. 0.68% aq. Copper as a tripeptide was applied on isolated stratum corneum, on heat-separated epidermis and on dermatomed skin. Receptor fluid collected over 48 h in 4 h intervals was analyzed by inductively coupled plasma mass spectrometry for copper in tissues and receptor fluid.
The permeability coefficient of the compound through dermatomed skin was 2.43 ± 0.51 × 10−4 cm/h; 136.2 ± 17.5 μg/cm2 copper permeated 1 cm2 of that tissue over 48 h, while 82 ± 8.1 μg/cm2 of copper were retained there as depot.
Applied tansdermally as the tripeptide on human skin ex vivo, copper permeated the skin and was also retained in skin tissue in amounts potentially effective for the treatment of inflammatory diseases
Erratum to: Human skin penetration of a copper tripeptide in vitro as a function of skin layer
Human skin penetration of a copper tripeptide in vitro as a function of skin layer
We study a set of 28 GRB light-curves detected between 15 December
2002 and 9 June 2003 by the anti-coincidence shield of the
spectrometer (SPI) of INTEGRAL. During this period it has detected
50 bursts, that have been confirmed by other instruments, with a
time resolution of 50 ms. First, we derive the basic
characteristics of the bursts: various duration measures, the
count peak flux and the count fluence. Second, a sub-sample of 11 bursts with 12 individual, well-separated pulses is studied. We
fit the pulse shape with a model by Kocevski et al. (2003)
and find that the pulses are quite self-similar in shape. There is
also a weak tendency for the pulses with steep power-law decays to
be more asymmetric. Third, the variability of the complex
light-curves is studied by analyzing their power-density-spectra
(PDS) and their RMS variability.
The averaged PDS, of the whole sample, is a power-law with index
of and a break between 1–2 Hz. Fourth, we also
discuss the background and noise levels. We found that the
background noise has a Gaussian distribution and its power is
independent of frequency, i.e., it is white noise. However, it
does not follow a Poisson statistic since on average the variance
is ~1.6 larger than the mean. We discuss our results in
context of the current theoretical picture in which GRBs are
created in an anisotropic, highly relativistic outflow from
collapsing massive stars. Finally, we note that the exact
behaviour of the instrument is not yet known and therefore the
above results should be treated as preliminary.
Human skin penetration of a copper tripeptide in vitro as a function of skin layer
Objective and designSkin retention and penetration by copper applied as glycyl-L-histidyl-L-lysine cuprate diacetate was evaluated in vitro in order to assess its potential for its transdermal delivery as an anti-inflammatory agent.Materials and methodsFlow-through diffusion cells with 1 cm(2) exposure area were used under infinite dose conditions. 0.68% aq. copper tripeptide as permeant was applied on isolated stratum corneum, heat-separated epidermis and dermatomed skin and receptor fluid collected over 48 h in 4 h intervals using inductively coupled plasma mass spectrometry to analyze for copper in tissues and receptor fluid.ResultsThe permeability coefficient of the compound through dermatomed skin was 2.43 ± 0.51 × 10(-4) cm/h; 136.2 ± 17.5 μg/cm(2) copper permeated 1 cm(2) of that tissue over 48 h, while 97 ± 6.6 μg/cm(2) were retained as depot.ConclusionsCopper as tripeptide was delivered in potentially therapeutically effective amounts for inflammatory disease
Human skin retention and penetration of a copper tripeptide in vitro as function of skin layer towards anti-inflammatory therapy
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Human skin retention and penetration of a copper tripeptide in vitro as function of skin layer towards anti-inflammatory therapy.
Objective and designThe skin retention and penetration characteristics of copper applied as glycyl-L-histidyl-L-lysine cuprate diacetate were evaluated in vitro in order to assess the potential for its transdermal delivery as anti-inflammatory agent.Materials and methodsFlow-through diffusion cells with 1 cm(2) exposure area were used under infinite dose conditions. 0.68% aq. Copper as a tripeptide was applied on isolated stratum corneum, on heat-separated epidermis and on dermatomed skin. Receptor fluid collected over 48 h in 4 h intervals was analyzed by inductively coupled plasma mass spectrometry for copper in tissues and receptor fluid.ResultsThe permeability coefficient of the compound through dermatomed skin was 2.43 ± 0.51 × 10(-4) cm/h; 136.2 ± 17.5 μg/cm(2) copper permeated 1 cm(2) of that tissue over 48 h, while 82 ± 8.1 μg/cm(2) of copper were retained there as depot.ConclusionsApplied tansdermally as the tripeptide on human skin ex vivo, copper permeated the skin and was also retained in skin tissue in amounts potentially effective for the treatment of inflammatory diseases
Recommended from our members
Human skin penetration of a copper tripeptide in vitro as a function of skin layer.
Objective and designSkin retention and penetration by copper applied as glycyl-L-histidyl-L-lysine cuprate diacetate was evaluated in vitro in order to assess its potential for its transdermal delivery as an anti-inflammatory agent.Materials and methodsFlow-through diffusion cells with 1 cm(2) exposure area were used under infinite dose conditions. 0.68% aq. copper tripeptide as permeant was applied on isolated stratum corneum, heat-separated epidermis and dermatomed skin and receptor fluid collected over 48 h in 4 h intervals using inductively coupled plasma mass spectrometry to analyze for copper in tissues and receptor fluid.ResultsThe permeability coefficient of the compound through dermatomed skin was 2.43 ± 0.51 × 10(-4) cm/h; 136.2 ± 17.5 μg/cm(2) copper permeated 1 cm(2) of that tissue over 48 h, while 97 ± 6.6 μg/cm(2) were retained as depot.ConclusionsCopper as tripeptide was delivered in potentially therapeutically effective amounts for inflammatory disease