41 research outputs found
Mcmbrane Potential as a Coupling Agent for Photophosphorylation by Bacteriorhodopsin and ATP-ase Containing Artificial Membrane
The steady state kinetic and thermodynamic properties of a Ā»minimalĀ« photosynthetic system containing two different proton
pumps in the planar artificial membrane have been considered.
The light activated proton pump, modelled after bacteriorhodopsin
(bR), and\u27 the ATP-using proton pump, modelled after Neurospora
ATP-ase, were interacting only through the common photopotential
developed by bR. In the stationary state of the illuminated coupled system the following properties were calculated by Hill\u27s diagram method: proton flux for each macromolecule, photopotential, total effective force, efficiency of free energy storage, efficiency of light free energy utilization, entropy production, and adenylate energy charge in the internal compartment
Ukupna taložna tvar na podruÄju Primorsko-goranske županije u razdoblju 1975.-2008.
Dustfall measurements in Primorsko-goranska county started in 1975 in the Bakar Bay (Site 2 - Bakar and Site 3 - Kraljevica). The measurements were extended to the city of Rijeka (Site 1 - Rijeka) in 1982, to the nearby islands (Site 4 - Krk and Site 5 - Cres) in 1986, and inland to Gorski Kotar (Site 6 - Delnice and Site 7 - Lividraga) in 1995. This article brings the results of dustfall measurements from 1975 to 2008. Dustfall was low in the city, the nearby islands, and Gorski Kotar. Recommended and limit values
were occasionally exceeded in the Bakar Bay due to emissions from the coke plant and harbour at Site 2 (1979-1997) and from the shipyard at Site 3. Lead content in the dustfall was below the national limits, save for two occasions at Site 2. Deposition of sulphur and nitrogen at the inland sites were below the respective critical load values.Ispitivanja ukupne taložne tvari u Primorsko-goranskoj županiji zapoÄela su 1975. godine na podruÄju Bakarskog zaljeva. Mjerenja su se 1982. god proÅ”irila na grad Rijeku, 1986. na otoke Krk i Cres, a 1995. i na podruÄje Gorskog kotara. U radu su prikazani rezultati mjerenja ukupne taložne tvari u razdoblju 1975.-2008. na sedam odabranih postaja: u gradu Rijeci, dvije na podruÄju Bakarskog zaljeva (Bakar i Kraljevica), dvije na otocima (Krk i Cres) te dvije u Gorskom kotaru (Delnice i Lividraga). Analizirano je kretanje prosjeÄnih godiÅ”njih vrijednosti ukupno istaloženih tvari te u njima prisutnih koliÄina sagorivog pepela, sulfata, nitrata, amonija i olova. Na podruÄju grada Rijeke, otoka Krka i Cresa te Gorskog kotara
koliÄina ukupne taložne tvari je niska. KoliÄine ukupne taložne tvari povremeno su prelazile preporuÄenu graniÄnu vrijednost u Bakru (1979.-1994.), zbog emisija iz koksare i iz luke za rasuti teret te u Kraljevici, gdje se osamdesetih godina osjeÄao utjecaj koksare u Bakru, a od sredine devedesetih pojaÄani rad u brodogradiliÅ”tu. KoliÄine olova u taložnoj tvari su niske na svim postajama, osim povremenih skokova u Bakru, kada su prijeÄene preporuÄene vrijednosti. KoliÄine istaloženog sumpora i duÅ”ika na podruÄju
Gorskog kotara ispod su odgovarajuÄih vrijednosti kritiÄnog optereÄenja za ispitivana podruÄja
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Numerical investigation of oil injection in a Roots blower operated as expander
The adoption of positive displacement machines as expanders in Organic Rankine Cycles (ORCs) is increasingly common, especially in the low to medium power range. At the same time, these devices often serve as compressor in Vapor-Compression Refrigeration Systems. In both cases, the application of Computation Fluid Dynamics (CFD) to optimize such machines has become an integrated tool in the design process. As a consequence, several challenges associated with the numerical simulation have to be taken into account. For example, the modeling of the gap represents a challenge for the stability of the numerical analysis. The dynamic of the process, combined with deformations of the clearances and of the working chamber has to be considered with extra care. To raise the efficiency of the machine, oil is typically injected. Its numerical modeling imply an extra challenge in the simulation of the actual operation of the machine. The present work is mainly focused on the multi-phase nature of the flow, with a particular analysis of the lubricant oil injected. In this work, a two-lobe Roots blower operated as expander has been simulated with the open-source software OpenFOAM-v1812, using the SCORG-V5.2.2. This analysis highlights the areas that are affected the most by the oil presence in order to highlight the sealing effect it provides
Mehanizam toksiÄnosti i detoksikacije organofosfornih spojeva s naglaskom na istraživanja u Hrvatskoj
This review comprises studies on the mechanisms of toxicity and detoxication of organophosphorus (OP) compounds done in Croatia in different research areas. One area is the synthesis of antidotes against OP poisoning and their in vivo testing in experimental animals. In vitro studies included in this review focus on the mechanisms of reversible inhibition of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), protection of cholinesterases from inhibition by OPs, and reactivation of phosphylated cholinesterases. The third area comprises distribution profiles of BChE and paraoxonase (PON) phenotypes in selected population groups and the detection of OPs and metabolites in humans. Finally, methods are described for the detection of OP compounds in human blood and other media by means of cholinesterase inhibitionPrikazana su istraživanja voÄena u Hrvatskoj na razliÄitim podruÄjima mehanizma toksiÄnosti i detoksikacije organofosfornih (OP) spojeva. Jedno je podruÄje sinteza antidota protiv otrovanja OP spojevima i testiranje in vivo antidota na eksperimentalnim životinjama. Istraživanja in vitro odnose se na mehanizam reverzibilne inhibicije acetilkolinesteraze (AChE) i buturilkolinesteraze (BChE), zaÅ”titu kolinesteraza od inhibicije OP spojevima te reaktivaciju fosfiliranih kolinesteraza. TreÄe je podruÄje distribucija fenotipova BChE i paraoksonaze (PON) u odabranim populacijama te detekcija OP spojeva i njihovih metabolita u ljudima. Na kraju su opisane metode detekcije OP spojeva u ljudskoj krvi i drugim medijima koje se osnivaju na inhibiciji kolinesteraza
Multi-genome identification and characterization of chlamydiae-specific type III secretion substrates: the Inc proteins
<p>Abstract</p> <p>Background</p> <p><it>Chlamydiae </it>are obligate intracellular bacteria that multiply in a vacuolar compartment, the inclusion. Several chlamydial proteins containing a bilobal hydrophobic domain are translocated by a type III secretion (TTS) mechanism into the inclusion membrane. They form the family of Inc proteins, which is specific to this phylum. Based on their localization, Inc proteins likely play important roles in the interactions between the microbe and the host. In this paper we sought to identify and analyze, using bioinformatics tools, all putative Inc proteins in published chlamydial genomes, including an environmental species.</p> <p>Results</p> <p>Inc proteins contain at least one bilobal hydrophobic domain made of two transmembrane helices separated by a loop of less than 30 amino acids. Using bioinformatics tools we identified 537 putative Inc proteins across seven chlamydial proteomes. The amino-terminal segment of the putative Inc proteins was recognized as a functional TTS signal in 90% of the <it>C. trachomatis </it>and <it>C. pneumoniae </it>sequences tested, validating the data obtained <it>in silico</it>. We identified a <it>macro </it>domain in several putative Inc proteins, and observed that Inc proteins are enriched in segments predicted to form coiled coils. A surprisingly large proportion of the putative Inc proteins are not constitutively translocated to the inclusion membrane in culture conditions.</p> <p>Conclusions</p> <p>The Inc proteins represent 7 to 10% of each proteome and show a great degree of sequence diversity between species. The abundance of segments with a high probability for coiled coil conformation in Inc proteins support the hypothesis that they interact with host proteins. While the large majority of Inc proteins possess a functional TTS signal, less than half may be constitutively translocated to the inclusion surface in some species. This suggests the novel finding that translocation of Inc proteins may be regulated by as-yet undetermined mechanisms.</p
Surface Energy Budgets of Arctic Tundra During Growing Season
This study analyzed summer observations of diurnal and seasonal surface energy budgets across several monitoring sites within the Arctic tundra underlain by permafrost. In these areas, latent and sensible heat fluxes have comparable magnitudes, and ground heat flux enters the subsurface during short summer intervals of the growing period, leading to seasonal thaw. The maximum entropy production (MEP) model was tested as an input and parameter parsimonious model of surface heat fluxes for the simulation of energy budgets of these permafrostāunderlain environments. Using net radiation, surface temperature, and a single parameter characterizing the thermal inertia of the heat exchanging surface, the MEP model estimates latent, sensible, and ground heat fluxes that agree closely with observations at five sites for which detailed flux data are available. The MEP potential evapotranspiration model reproduces estimates of the PenmanāMonteith potential evapotranspiration model that requires at least five input meteorological variables (net radiation, ground heat flux, air temperature, air humidity, and wind speed) and empirical parameters of surface resistance. The potential and challenges of MEP model application in sparsely monitored areas of the Arctic are discussed, highlighting the need for accurate measurements and constraints of ground heat flux.Plain Language SummaryGrowing season latent and sensible heat fluxes are nearly equal over the Arctic permafrost tundra regions. Persistent ground heat flux into the subsurface layer leads to seasonal thaw of the top permafrost layer. The maximum energy production model accurately estimates the latent, sensible, and ground heat flux of the surface energy budget of the Arctic permafrost regions.Key PointThe MEP model is parsimonious and well suited to modeling surface energy budget in dataāsparse permafrost environmentsPeer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/150560/1/jgrd55584.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/150560/2/jgrd55584_am.pd
Polycyclic Aromatic Hydrocarbons in Air
PolicikliÄki aromatski ugljikovodici (PAU) spojevi su koji se sastoje od dva ili viÅ”e kondenziranih aromatskih prstenova. Nastaju prilikom nepotpunog izgaranja ili pirolize organskih tvari. Dokazano je da neki PAU imaju kancerogena, a neki mutagena svojstva, pa je stoga potrebno kontinuirano pratiti koncentracije PAU u zraku, vodi i tlu, te pokuÅ”ati sanirati izvore PAU. PAU s dva ili tri aromatska prstena postojani su u plinovitoj fazi, dok se PAU s viÅ”e aromatskih prstenova nalaze u zraku uglavnom vezani na Äestice. Visoke koncentracije PAU prisutne su u atmosferi urbanih podruÄja, a najviÅ”e su zimi kada su pojaÄane emisije iz kuÄnih ložiÅ”ta. U ljetno doba koncentracije su niže jer je veÄina policikliÄkih aromatskih ugljikovodika nestabilna na visokim temperaturama, a osim toga dolazi do njihove oksidacije i fotooksidacije. Metode mjerenja PAU u zraku ukljuÄuju uzorkovanje prosisavanjem na filterski papir ili kruti adsorbens, ekstrakciju i kromatografsku analizu. U ovom radu prikazani su rezultati mjerenja benzo[a]pirena (BaP) kao glavnog predstavnika PAU na nekim lokacijama u svijetu. Razine BaP u svijetu usporeÄene su s rezultatima mjerenja provedenim do sada u Hrvatskoj.Polycyclic aromatic hydrocarbons (PAHs) are a large group of organic compounds consisting of two or more condense aromatic rings. They are products of incomplete combustion or pyrolysis of organic matter. Because some PAHs such as Benzo[a]pyrene (BaP) are proven carcinogens and mutagens, it is necessary to continuously monitor their concentrations in the air, water, and soil. PAHs with two or three aromatic rings are stable in the gas phase, while most PAHs with five or more aromatic rings bond to particles. Higher concentrations of PAHs are present in the atmosphere of urban areas, mostly in the winter, due to heating. In the summer, these concentrations drop because most PAHs are unstable at high temperatures and break down by oxidation and photooxidation. Measurements of PAHs in the air include sampling on the filter paper or solid adsorbent, extraction, and chromatographic analysis. This review presents the measurements of BaP in some locations in the world and compares them with the findings in Croatia