Bacteria homologus to Aeromonas capable of microcystin degradation

Water blooms dominated by cyanobacteria are capable of producing hepatotoxins known as microcystins. These toxins are dangerous to people and to the environment. Therefore, for a better understanding of the biological termination of this increasingly common phenomenon, bacteria with the potential to degrade cyanobacteria-derived hepatotoxins and the degradative activity of culturable bacteria were studied. Based on the presence of the mlrA gene, bacteria with a homology to the Sphingopyxis and Stenotrophomonas genera were identified as those presenting potential for microcystins degradation directly in the water samples from the Sulejów Reservoir (SU, Central Poland). However, this biodegrading potential has not been confirmed in in vitro experiments. The degrading activity of the culturable isolates from the water studied was determined in more than 30 bacterial mixes. An analysis of the biodegradation of the microcystin-LR (MC-LR) together with an analysis of the phylogenetic affiliation of bacteria demonstrated for the first time that bacteria homologous to the Aeromonas genus were able to degrade the mentioned hepatotoxin, although the mlrA gene was not amplified. The maximal removal efficiency of MC-LR was 48%. This study demonstrates a new aspect of interactions between the microcystin-containing cyanobacteria and bacteria from the Aeromonas genus.The authors would like to acknowledge the European Cooperation in Science and Technology, COST Action ES 1105 “CYANOCOST - Cyanobacterial blooms and toxins in water resources: Occurrence, impacts and management” for adding value to this study through networking and knowledge sharing with European experts and researchers in the field. The Sulejów Reservoir is a part of the Polish National Long- Term Ecosystem Research Network and the European LTER site

Biodiversity conservation and monitoring : engagement and motivations of citizen scientists

Biodiversity conservation cannot operate in Central Eastern European countries without a well-established monitoring system, that is dependent on the citizen scientists input. Here we analyse, based on a Polish case: (1) The contribution of NGOs to the national nature monitoring scheme and their collaboration with governmental and scientifi c institutions and (2) the motivation of citizen scientists to volunteer for NGOs’ monitoring activities. The study comprises a focus group interview, 30 in-depth interviews with coordinators, citizen scientists, experts and a 23 days long participant observation of a model NGO. We have assessed the monitoring input of NGOs as being a contributory factor infl uencing the biodiversity conservation effectiveness. The cooperation between governmental, scientifi c institutions and NGOs exists, but is dependent on national funding. Although NGOs highlight the lack of coherence in monitoring methodology, they are willing to join the biodiversity monitoring, especially at the European Ecological Network – Natura 2000 sites. On the other hand the trust concerning cooperation with citizen scientists is limited. However, despite this, they still turned out to be trustworthy partners. The most effective way to maintain cooperation with citizen scientists is to create a bond in a group and to provide them with the opportunity to develop their passion for nature. Our fi ndings have shed light on the growing importance of citizen scientists in biodiversity governance, providing recommendations for developBiodiversity conservation cannot operate in Central Eastern European countries without a well-established monitoring system, that is dependent on the citizen scientists input. Here we analyse, based on a Polish case: (1) The contribution of NGOs to the national nature monitoring scheme and their collaboration with governmental and scientifi c institutions and (2) the motivation of citizen scientists to volunteer for NGOs’ monitoring activities. The study comprises a focus group interview, 30 in-depth interviews with coordinators, citizen scientists, experts and a 23 days long participant observation of a model NGO. We have assessed the monitoring input of NGOs as being a contributory factor infl uencing the biodiversity conservation effectiveness. The cooperation between governmental, scientifi c institutions and NGOs exists, but is dependent on national funding. Although NGOs highlight the lack of coherence in monitoring methodology, they are willing to join the biodiversity monitoring, especially at the European Ecological Network – Natura 2000 sites. On the other hand the trust concerning cooperation with citizen scientists is limited. However, despite this, they still turned out to be trustworthy partners. The most effective way to maintain cooperation with citizen scientists is to create a bond in a group and to provide them with the opportunity to develop their passion for nature. Our fi ndings have shed light on the growing importance of citizen scientists in biodiversity governance, providing recommendations for development of the effective monitoring schemes based on the volunteer work of citizen scientists.ment of the effective monitoring schemes based on the volunteer work of citizen scientists

Emerging multilevel environmental governance – A case of public participation in Poland

AbstractIn recent decades, nature conservation policies have increasingly considered the participation of various actors. However, the effectiveness of such efforts is often questionable, and better methods of engaging stakeholders are still being sought. In this paper, we present an analysis of a consultation program conducted in the final stage of site selection for Natura 2000 in Małopolska, a region located in southern Poland. Based on a desk study and qualitative research, we analysed the modes and degrees of participation, the normative foundations of the consultation program, and the goals and expectations that characterise participants. The results are discussed using Unnestall's and Arnstein's typologies of participation, which show the limited success of the participatory process in representing all relevant stakeholders and enabling their actual influence on final decisions. The importance of implementing the EU directives for emerging multilevel governance in the nature conservation sector in Central and Eastern Europe, including Poland, is highlighted. In the context of Poland, the consultation program analysed appeared to be a novel and innovative step forward towards the development of a meaningful participatory approach in this region of Europe

A critical assessment of a protected area conflict analysis based on secondary data in the age of datafication

Recently, a global trend towards a broader use of secondary data in social sciences has been reinforced by the COVID-19 pandemic. This evoked doubts about the validity of the results unless restrictive assessment procedures are implemented. To address this need in the field of protected area (PA) conflict analysis, we propose a three-fold approach (theory-, method-, and cross-scale simulation-driven) to assess the usefulness of the utilized state register dataset and the indicator analysis methodology for the multi-level recognition of PA conflict determinants. With the ultimate aim to inform case study selection, we processed 187 relevant indicators from the official Statistics Poland register for a Lesser Poland region. We distinguished five types of PA conflict determinants in Lesser Poland (‘urbanity’, ‘agriculture’, ‘tourism’, ‘small-scale entrepreneurship’, and ‘sprawl’) and respective groups of 15 clusters comprising local-level units. For one cluster, we juxtaposed the obtained results with secondary data from another source (Internet content) and for a specific PA (Tatra National Park). Although the reported conflict issues corresponded to the indicator-derived descriptors of the cluster, in the theory-driven phase of the assessment, the state register failed to address the key prerequisites of PA conflicts. We have demonstrated that, in crisis conditions such as COVID-19, the proposed method can serve as a proxy for a multi-level recognition of PA conflict potentials, provided that it synthesises the results of different methodological approaches, followed by in-person interviews in the selected case studies

Client depletion assay comparison of paclitaxel to Hsp90 inhibitors

Many researchers have claimed they have found a successful inhibitor of Hsp90; however, we suspect they are only successful when the inhibitor is used in large quantities. On top of being one of the easiest ways to show an effective inhibitor, researchers are claiming they have found an inhibitor when only meeting as few as two hallmarks. Due to the researchers' inhibitors only meeting two of the criteria, we are trying to show whether these limited expectations are a valid way in identifying Hsp90 inhibitors. We think the client depletion assay test is not specific to Hsp90 inhibitors but instead is a generalized cell response to cell death. At this point in our research, we know a lot about client depletion assays and Hsp90s; however, we do not know if the claims these researchers are making are valid claims because of the amount of Hsp90 inhibitor they are using.Howard Hughes Medical Institute Science Education ProgramBiochemistry and Molecular Biolog

The use of Yttrium-90 Ibritumomab Tiuxetan (^{90}Y-IT) as a consolidation therapy in high-risk patients with diffuse large B-cell lymphoma ineligible for autologous stem-cell transplantation

AIM OF THE STUDY: To evaluate the efficacy and safety of Yttrium-90 Ibritumomab Tiuxetan ((90)Y-IT) as a consolidation therapy in the management of DLBCL. MATERIAL AND METHODS: Patients with primary refractory or high-risk DLBCL (n = 18), ineligible for autologous stem-cell transplantation, were included in a retrospective study performed at three centers by the Polish Lymphoma Research Group (PLRG). All patients (mean age 61, range 35–82) either didn't achieve a complete response or didn't complete the scheduled therapy due to its complications. Response rates (CR, PR, SD, PD) according to Cheson criteria, overall survival (OS), progression-free survival (PFS) and adverse effects of radioimmunotherapy were analyzed. RESULTS: Consolidation radioimmunotherapy increased the CR rate from 38% (n = 7) to 82% (n = 15). One patient remained in PR, one patient remained in SD, while one patient remained in PD. During a median follow-up of five years, 11 patients (62%) were alive with no recurrence, 4 patients (22%) were alive with relapse while 3 patients (16%) died. There was no statistically significant difference in PFS between those in CR and those in PR before (90)Y-IT. CONCLUSIONS: Radioimmunotherapy is an effective consolidation therapy for high risk/refractory DLBCL patients and worthy of further investigation in prospective trials

Postmodernism is old, let us go further : a pamphlet against decostructionism, constructivism, relativism, and methodological anarchism

This paper means to be a collective and intercultural pamphlet against the postmodern approach to knowledge. However, our criticism - mainly directed at deconstructionism, constructivism, relativism and methodological anarchism - is not grounded in old positivistic philosophy. We are young scholars and students whose teachers were mainly postmodernists, therefore we accept some of their ideas, but we also feel it necessary to stress the many limits of this approach and its inadequacy to respond to the challenges of the present day. From our perspective, postmodernism is not a new approach that must be simply studied, but an old one that needs to be surpassed. One of the merits of postmodernism is that it showed that positivism was too dogmatic and optimistic with regards to the progress of knowledge and civilization. However, in responding to positivism, postmodernism has gone too far in producing skepticism and pessimism. In addition, postmodernism is paradoxical when rejecting the categories of truth and progress, while still considering itself to be an approach better than positivism. Up to now the argument used to defend its preferability was its novelty. But an idea that is forty years old and was already anticipated at the beginning of the twentieth century cannot be treated as new. For all of these reasons this paper should be seen as one of the first examples of post-postmodern thought