BIOLOGICAL VALUE OF PUNY FRUITS RELATED TO THEIR ANTIRADICAL ACTIVITY

All analysed species of puny fruits (red currant (Ribes rubrum L) variant Jonkheervan Tets, white currant (Ribes vulgare L.) variant Blanka, black currant (Ribesnigrum L.) variant Eva, blueberry (Vaccinium myrtilis) variant Berkeley, elderberry(Sambucus nigra L.) variant Sambo, hawthorn (Crataegus oxyacantha), mulberry(Morus nigra L.) genotypes M152 and M047) are natural sources of anthocyanpigments and vitamin C with a high antiradical activity. Due to the fact that thehighest antiradical activity is not accompanied by the highest content of anthocyansand vitamin C in puny fruits, we suppose that the antiradical activity of plantmaterials is also connected with the presence of other compounds with antioxidantand antiradical activity. From our results follows that all studied puny fruits with ahigh antiradical activity increase the antioxidant value of human nutrition and alsoits prophylactic and medicinal effect

Increasing of selenium content and qualitative parameters in garden pea (Pisum sativum L.) after its foliar application

The presented paper deals with monitoring of the ways of selenium (Se) content increase by foliar biofortification with inorganic Se in two varieties of garden pea Pisum sativum L. (Premium, Ambassador) in the conditions of south Slovakia in 2014–2015. The results of experiments show that treatment of plants with two doses of Se concentration (50 g and 100 g Se ha–1 in the form of a sodium selenate anhydrous solution) at the flowering stage significantly increased the total Se content in the seeds of both varieties. Following the results by the consumption of 25 g of dried seeds of peas or 100 g of fresh pea seeds after the biofortification with 100 g Se ha–1 a recommended daily dose of Se in humans may be covered. The significantly positive influence of Se application on the total polyphenols content (TPC) has been confirmed in the both varieties after application of dosage in 100 g Se ha–1 (52% and 33%). A significant increase in the average value of total antioxidant capacity (TAC by DPPH method) in garden pea var. Ambassador was observed after the application of both doses of Se, in case of Premium variety only after application with a 100 g Se ha–1. Significantly increasing level of TAC by PCL (photochemiluminescence) method was found out only in case of var. Premium

Failure Behaviour of Fe- and Co-Based Amorphous Soft Magnetic Ribbons

Fractographic analysis of Co- and Fe-based high strength soft magnetic materials showed the high localization of the plastic deformation and the ductility in narrow shear bands. The estimated fracture toughness of these materials is closely associated with the scale of the local plastic zone

Structural Stability of Amorphous Alloy of Modified Finemet Type

The Finemet type amorphous alloys are well known as high frequency soft magnetic materials. They have good soft magnetic properties which are characterized by low coercive force and high permeability because of the lack of crystalline anisotropy. The structural stability of the amorphous ribbon of Finemet type modified by Mn, Al and Cr prepared by melt-spinning process was studied using differential scanning calorimetry and dynamical mechanical analysis. With increase of the crystalline portion in the sample, the Curie transition is shifted to the higher temperatures. The magnetic relaxation processes at frequencies above 10⁴ Hz were detected by mass magnetic susceptibility measurement

Structural Stability of Amorphous Alloy of Modified Finemet Type

The Finemet type amorphous alloys are well known as high frequency soft magnetic materials. They have good soft magnetic properties which are characterized by low coercive force and high permeability because of the lack of crystalline anisotropy. The structural stability of the amorphous ribbon of Finemet type modified by Mn, Al and Cr prepared by melt-spinning process was studied using differential scanning calorimetry and dynamical mechanical analysis. With increase of the crystalline portion in the sample, the Curie transition is shifted to the higher temperatures. The magnetic relaxation processes at frequencies above 10⁴ Hz were detected by mass magnetic susceptibility measurement

Nanoindentation in Metallic Glasses with Different Plasticity

Nanoindentation and thermomechanical experiments on three types of metallic glasses with different glass forming ability were carried out. The nanoindentation behaviour at room temperature was associated with the creep at elevated temperatures. Different discontinuity populations and their shape observed on the nanoindentation loading curves were compared with morphology of plastic deformed indent regions. The influence of the differences in thermal stability of studied alloys on the nanoindentation in these alloys were studied as well

Nanoindentation Study of the Influence of the Loading Rate on the Deformation of Metallic Glasses

Nanoindentation experiments at the loading rates from 0.05 to 100 mN.s-1 on the amorphous FeNiB alloy were executed. We found that the serrations in the load-displacement (P-h) curve are more pronounced at lower loading rates and gradually disappear upon increasing loading rate. We have estimated the contribution of the inhomogeneous plastic deformation from pop-in events on the P-h curves. The pop-in population was compared with the morphology of indents

Purification of replicating pancreatic β-cells for gene expression studies

β-cell proliferation is a rare event in adult pancreatic islets. To study the replication-related β-cell biology we designed a replicating β-cells sorting system for gene expression experiments. Replicating β-cells were identified by EdU incorporation and purified by flow cytometry. For β-cell separation islet cells were sorted by size, granularity and Newport Green fluorescence emission that was combined with emitted fluorescence for EdU-labelled replicating cells sorting. The purity of the resulting sorted populations was evaluated by insulin staining and EdU for β-cell identification and for replicating cells, respectively. Total RNA was isolated from purified cell-sorted populations for gene expression analysis. Cell sorting of dispersed islet cells resulted in 96.2% purity for insulin positivity in the collected β-cell fraction and 100% efficiency of the EdU-based cell separation. RNA integrity was similar between FACS-sorted replicating and quiescent β-cells. Global transcriptome analysis of replicating vs quiescent β-cells showed the expected enrichment of categories related to cell division and DNA replication. Indeed, key genes in the spindle check-point were the most upregulated genes in replicating β-cells. This work provides a method that allows for the isolation of replicating β-cells, a very scarce population in adult pancreatic islets