Cholesterol-directed nanoparticle assemblies based on single amino acid peptide mutations activate cellular uptake and decrease tumor volume.

Peptide drugs have been difficult to translate into effective therapies due to their low in vivo stability. Here, we report a strategy to develop peptide-based therapeutic nanoparticles by screening a peptide library differing by single-site amino acid mutations of lysine-modified cholesterol. Certain cholesterol-modified peptides are found to promote and stabilize peptide α-helix formation, resulting in selectively cell-permeable peptides. One cholesterol-modified peptide self-assembles into stable nanoparticles with considerable α-helix propensity stabilized by intermolecular van der Waals interactions between inter-peptide cholesterol molecules, and shows 68.3% stability after incubation with serum for 16 h. The nanoparticles in turn interact with cell membrane cholesterols that are disproportionately present in cancer cell membranes, inducing lipid raft-mediated endocytosis and cancer cell death. Our results introduce a strategy to identify peptide nanoparticles that can effectively reduce tumor volumes when administered to in in vivo mice models. Our results also provide a simple platform for developing peptide-based anticancer drugs

Cognitive Reappraisal in Children: Neuropsychological Evidence of Up-Regulating Positive Emotion From an ERP Study

Emotion regulation is a critical mechanism in the socio-emotional development of children. Previous studies revealed that children use cognitive reappraisal to downregulate negative emotions. Moreover, the amplitude of late positive potential (LPP) shows a more obvious reduction following neutral interpretations than following negative interpretations. However, whether children can use cognitive reappraisal to regulate positive emotions remains unclear. In the present study, 46 8- to 12-year-old children were asked to reappraise the meaning of pleasant pictures. Electroencephalography (EEG) data were collected during the task. As predicted, LPP amplitudes increased more following reappraisal condition than following pleasant condition. The analysis of spatial-temporal shifting patterns showed that the effect occurred in the earlier window for the posterior region. As time progressed, this effect evidenced a trend from posterior region to the central and anterior regions, especially for the younger children. Furthermore, the greater brain activations occurred in left hemisphere when children upregulated positive emotions which partially supported previous research suggesting that increasing positive emotion engaged primarily left-lateralized prefrontal regions. Taken together, the findings suggest that children can use cognitive reappraisal to upregulate positive emotions

Perception of Western Musical Modes: A Chinese Study

The major mode conveys positive emotion, whereas the minor mode conveys negative emotion. However, previous studies have primarily focused on the emotions induced by Western music in Western participants. The influence of the musical mode (major or minor) on Chinese individuals’ perception of Western music is unclear. In the present experiments, we investigated the effects of musical mode and harmonic complexity on psychological perception among Chinese participants. In Experiment 1, the participants (N = 30) evaluated 24 musical excerpts in five dimensions (pleasure, arousal, dominance, emotional tension, and liking). In Experiment 2, the participants (N = 40) evaluated 48 musical excerpts. Perceptions of the musical excerpts differed significantly according to mode, even if the stimuli were Western musical excerpts. The major-mode music induced greater pleasure and arousal and produced higher liking ratings than the minor-mode music, whereas the minor-mode music induced greater tension than the major-mode music. Mode did not influence the dominance rating. Perception of Western music was not influenced by harmonic complexity. Moreover, preference for musical mode was influenced by previous exposure to Western music. These results confirm the cross-cultural emotion induction effects of musical modes in Western music

Influence of male’s facial attractiveness, vocal attractiveness and social interest on female’s decisions of fairness

Abstract The present study employed dictator game and ultimatum game to investigate the effect of facial attractiveness, vocal attractiveness and social interest in expressing positive (“I like you”) versus negative signals (“I don’t like you”) on decision making. Female participants played against male recipients in dictator game and ultimatum game while played against male proposers in ultimatum game. Results showed that participants offered recipients with attractive faces more money than recipients with unattractive faces. Participants also offered recipients with attractive voices more money than recipients with unattractive voices, especially under the positive social interest condition. Moreover, participants allocated more money to recipients who expressed positive social interest than those who expressed negative social interest, whereas they would also expect proposers who expressed positive social interest to offer them more money than proposers who expressed negative social interest. Overall, the results inform beauty premium for faces and voices on opposite-sex economic bargaining. Social interest also affects decision outcomes. However, the beauty premium and effect of social interest varies with participants’ roles

Differences of Affective Learning with OwnRace and OtherRace Faces An EyeTracking Study

Minimal affective learning is a phenomenon wherein people can learn about the affective meaning of other people with brief behavioral descriptions. Prior research mainly focused on affective learning with own-race faces. Own-race bias is a robust phenomenon describing that people can recognize own-race faces more efficiently than other-race faces. In the current study, we investigated whether own-race bias would influence minimal affective learning. Chinese participants learned Chinese and Caucasian faces paired with behaviors of different valence. After learning, they were asked to evaluate the learned faces and novel faces. Their eye movements and pupil diameters were continuously monitored during the experiment. We analyzed the change in pupil dilation to assess how much cognitive effort was required for affective learning. The results showed that participants only learned positive information with faces. Learning performance for other-race faces was similar with own-race faces. In addition, change of pupil dilation was larger when learning other-race than own-race faces, suggesting a greater cognitive effort for affective learning with other-race faces. Taken together, the results demonstrated that affective learning for other-race faces was more difficult than own-race faces. This research provided more support for the notion that different cognitive strategies were employed by faces of different race

The Effects of Live Streamer’s Facial Attractiveness and Product Type on Consumer Purchase Intention: An Exploratory Study with Eye Tracking Technology

As a booming branch of online retailing, live-streaming e-commerce can present abundant information dimensions and diverse forms of expression. Live-streaming e-commerce has enabled online retailers to interact with customers face-to-face, resulting in widespread instances of emotional and impulse buying behavior. Prior research in live-streaming e-commerce has suggested that live streamers’ characteristics, especially the live streamer’s face, can affect customers’ purchase intentions. The present research used questionnaire surveys and an eye tracking experiment to investigate the impact of live streamer’s facial attractiveness on consumer purchase intention for search-based and experience-based products. The questionnaire survey analyzed 309 valid questionnaires and revealed that attractive faces are the key influencing factor driving consumers’ impulse purchase intentions. Moreover, consumers’ emotional experience plays a partial mediating role in the process of live streamers’ faces influencing purchase intention. The eye tracking experiment further explored the mechanism of a live streamer’s facial attractiveness on consumers’ purchase intentions of search-based products and experience-based products from the perspective of visual attention by analyzing 64 valid sets of data. The results showed that attractive faces attract more consumers’ attention and, therefore, increase their purchase intention. Furthermore, there is a significant interaction between product type, the live streamer’s facial attractiveness, and consumers’ purchase intentions. In the case of unattractive live streamers, consumers are more likely to buy search-based products than experience-based products, while the purchase intention does not vary between search-based products and experience-based products in the case of attractive live streamers. The present study provides evidence for ‘beauty premium’ in live-streaming e-commerce and sheds light on the design of the match between live streamers and different types of products

Who Learns More? Cultural Differences in Implicit Sequence Learning

Background: It is well documented that East Asians differ from Westerners in conscious perception and attention. However, few studies have explored cultural differences in unconscious processes such as implicit learning. Methodology/Principal Findings: The global-local Navon letters were adopted in the serial reaction time (SRT) task, during which Chinese and British participants were instructed to respond to global or local letters, to investigate whether culture influences what people acquire in implicit sequence learning. Our results showed that from the beginning British expressed a greater local bias in perception than Chinese, confirming a cultural difference in perception. Further, over extended exposure, the Chinese learned the target regularity better than the British when the targets were global, indicating a global advantage for Chinese in implicit learning. Moreover, Chinese participants acquired greater unconscious knowledge of an irrelevant regularity than British participants, indicating that the Chinese were more sensitive to contextual regularities than the British. Conclusions/Significance: The results suggest that cultural biases can profoundly influence both what people consciously perceive and unconsciously learn

Negative Affect Reduces Performance in Implicit Sequence Learning

Background: It is well documented that positive rather than negative moods encourage integrative processing of conscious information. However, the extent to which implicit or unconscious learning can be influenced by affective states remains unclear. Methodology/Principal Findings: A Serial Reaction Time (SRT) task with sequence structures requiring integration over past trials was adopted to examine the effect of affective states on implicit learning. Music was used to induce and maintain positive and negative affective states. The present study showed that participants in negative rather than positive states learned less of the regularity. Moreover, the knowledge was shown by a Bayesian analysis to be largely unconscious as participants were poor at recognizing the regularity. Conclusions/Significance: The results demonstrated that negative rather than positive affect inhibited implicit learning of complex structures. Our findings help to understand the effects of affective states on unconscious or implicit processing