Supplementary material for the article: Fukushi, K.; Fujita, Y.; Nonogaki, J.; Tsujimoto, J.-I.; Hattori, T.; Inui, H.; Beškoski, V. P.; Hotta, H.; Hayashi, M.; Nakano, T. Capillary Zone Electrophoresis Determination of Fluoride in Seawater Using Transient Isotachophoresis. Analytical and Bioanalytical Chemistry 2018, 410 (6), 1825–1831. https://doi.org/10.1007/s00216-017-0838-0

Supplementary material for: [https://doi.org/10.1007/s00216-017-0838-0]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2083

Role of the VEGF-Flt-1-FAK pathway in the pathogenesis of osteoclastic bone destruction of giant cell tumors of bone

BACKGROUND: Giant cell tumors (GCTs) of bone are primary benign bone tumors that are characterized by a high number of osteoclast-like multinuclear giant cells (MNCs). Recent studies suggest that the spindle-shaped stromal cells in GCTs are tumor cells, while monocyte-like cells and MNCs are reactive osteoclast precursor cells (OPCs) and osteoclasts (OCs), respectively. In this study, we investigated the pathogenesis of osteoclastic bone destruction in GCTs by focusing on the role of the vascular endothelial growth factor (VEGF)-Flt-1 (type-1 VEGF receptor)-focal adhesion kinase (FAK) pathway. METHODS: The motility of OPCs cells was assessed by a chemotaxis assay and the growth of OPCs was examined using a cell proliferation assay. The expression of VEGF and activation of Flt-1 and FAK in clinical GCT samples and in OPCs were detected by immunohistochemistry and immunoblotting. The correlation between the expression levels of activated Flt-1 and FAK and clinical stages of GCTs was investigated by immunohistochemistry. RESULTS: In GCT samples, CD68, a marker of OPCs and OCs, co-localized with Flt-1. Conditioned media from GCT tissue (GCT-CM) enhanced the chemotaxis and proliferation of OPCs. GCT-CM also stimulated FAK activation in OPCs in vitro. Moreover, there was a correlation between the clinical stage of GCTs and the expression of tyrosine-phosphorylated Flt-1 and FAK. CONCLUSIONS: Our results suggest that the VEGF-Flt-1-FAK pathway is involved in the pathogenesis of bone destruction of GCTs

Supplementary material for the article: Fukushi, K.; Fujita, Y.; Nonogaki, J.; Tsujimoto, J.-I.; Hattori, T.; Inui, H.; Beškoski, V. P.; Hotta, H.; Hayashi, M.; Nakano, T. Capillary Zone Electrophoresis Determination of Fluoride in Seawater Using Transient Isotachophoresis. Analytical and Bioanalytical Chemistry 2018, 410 (6), 1825–1831. https://doi.org/10.1007/s00216-017-0838-0

Supplementary material for: [https://doi.org/10.1007/s00216-017-0838-0]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2083

NG2 Proteoglycan Promotes Endothelial Cell Motility and Angiogenesis via Engagement of Galectin-3 and α3β1 Integrin

The NG2 proteoglycan is expressed by microvascular pericytes in newly formed blood vessels. We have used in vitro and in vivo models to investigate the role of NG2 in cross-talk between pericytes and endothelial cells (EC). Binding of soluble NG2 to the EC surface induces cell motility and multicellular network formation in vitro and stimulates corneal angiogenesis in vivo. Biochemical data demonstrate the involvement of both galectin-3 and α3β1 integrin in the EC response to NG2 and show that NG2, galectin-3, and α3β1 form a complex on the cell surface. Transmembrane signaling via α3β1 is responsible for EC motility and morphogenesis in this system. Galectin-3–dependent oligomerization may potentiate NG2-mediated activation of α3β1. In conjunction with recent studies demonstrating the early involvement of pericytes in angiogenesis, these data suggest that pericyte-derived NG2 is an important factor in promoting EC migration and morphogenesis during the early stages of neovascularization

Bone Mineral Density in Yusho Health Examination

特集号 油症とPCB及びダイキシン関連化合物 研究報告 第25集 責任編集者 古江増隆The Twenty-fifth Reports of the Study on Yusho―PCBs and Dioxin-Related Compounds―Editor Furue MasutakaWe examined the bone mineral density in Yusho patients. 460 subjects underwent dual-energy Xray absorptiometry bone scans as part of the nationwide Yusho health examination in 2011. Serum levels of polychlorinated dibenzo-p-dioxin, polychlorinated dibenzofurans, and non-ortho polychlorinated biphenyls were measured using high-resolution gas chromatography and high-resolution mass spectrometry. The average age was 64.0 years in women and 63.4 in men. About one-third (35％) of women (n=89) had low bone mineral density (BMD), defined as a T-score less than 70, while only 4.9％ of men (n=10) had low BMD. Serum levels of several congeners were positively associated with Z-scores in simple regression analysis. Further multivariate linear regression analysis should be required in the future