Physical Therapy Management Of A Patient After Hemorrhagic Stroke Using A Task-Oriented Approach In A Skilled Nursing Facility: A Case Report

Stroke is the leading cause of long term disability in the U.S.; nearly 800,000 Americans have a stroke each year. Subarachnoid hemorrhagic stroke occurs when one of the blood vessels in the brain bursts causing a release of blood which increases intracranial pressure. There is a lack of rehabilitation research in the skilled nursing setting for hemorrhagic stroke. The purpose of this case report is to describe the PT management, using a task-oriented approach, of a patient with a subarachnoid hemorrhagic stroke being treated in a skilled nursing setting.https://dune.une.edu/pt_studcrposter/1104/thumbnail.jp

Genetic Variation of 25 Y-Chromosomal and 15 Autosomal STR Loci in the Han Chinese Population of Liaoning Province, Northeast China

<div><p>In the present study, we investigated the genetic characteristics of 25 Y-chromosomal and 15 autosomal short tandem repeat (STR) loci in 305 unrelated Han Chinese male individuals from Liaoning Province using AmpFISTR^® Yfiler^® Plus and Identifiler^TM PCR amplification kits. Population comparison was performed between Liaoning Han population and different ethnic groups to better understand the genetic background of the Liaoning Han population. For Y-STR loci, the overall haplotype diversity was 0.9997 and the discrimination capacity was 0.9607. Gene diversity values ranged from 0.4525 (DYS391) to 0.9617 (DYS385). <i>Rst</i> and two multi-dimensional scaling plots showed that minor differences were observed when the Liaoning Han population was compared to the Jilin Han Chinese, Beijing Han Chinese, Liaoning Manchu, Liaoning Mongolian, Liaoning Xibe, Shandong Han Chinese, Jiangsu Han Chinese, Anhui Han Chinese, Guizhou Han Chinese and Liaoning Hui populations; by contrast, major differences were observed when the Shanxi Han Chinese, Yunnan Bai, Jiangxi Han Chinese, Guangdong Han Chinese, Liaoning Korean, Hunan Tujia, Guangxi Zhuang, Gansu Tibetan, Xishuangbanna Dai, South Korean, Japanese and Hunan Miao populations. For autosomal STR loci, DP ranged from 0.9621 (D2S1338) to 0.8177 (TPOX), with PE distributing from 0.7521 (D18S51) to 0.2988 (TH01). A population comparison was performed and no statistically significant differences were detected at any STR loci between Liaoning Han, China Dong, and Shaanxi Han populations. The results showed that the 25 Y-STR and 15 autosomal STR loci in the Liaoning Han population were valuable for forensic applications and human genetics, and Liaoning Han was an independent endogenous ethnicity with a unique subpopulation structure.</p></div

Genetic distances between all pairs of populations (Phylip software according to Nei).

<p>Genetic distances between all pairs of populations (Phylip software according to Nei).</p

A cluster structure based on haplotypes of the Liaoning Han population in the YHRD database.

<p>A cluster structure based on haplotypes of the Liaoning Han population in the YHRD database.</p

Rst values for pairwise comparisons between populations.

<p>Rst values for pairwise comparisons between populations.</p

A MDS plot based on Rst between the Liaoning Han population and five reference populations (the Liaoning Han population is marked with yellow).

<p>A MDS plot based on Rst between the Liaoning Han population and five reference populations (the Liaoning Han population is marked with yellow).</p

A MDS plot based on Rst between the Liaoning Han population and 22 East Asian populations (the Liaoning Han population is marked with yellow).

<p>A MDS plot based on Rst between the Liaoning Han population and 22 East Asian populations (the Liaoning Han population is marked with yellow).</p

Programmable Resistive-Switch Nanowire Transistor Logic Circuits

Programmable logic arrays (PLA) constitute a promising architecture for developing increasingly complex and functional circuits through nanocomputers from nanoscale building blocks. Here we report a novel one-dimensional PLA element that incorporates resistive switch gate structures on a semiconductor nanowire and show that multiple elements can be integrated to realize functional PLAs. In our PLA element, the gate coupling to the nanowire transistor can be modulated by the memory state of the resistive switch to yield programmable active (transistor) or inactive (resistor) states within a well-defined logic window. Multiple PLA nanowire elements were integrated and programmed to yield a working 2-to-4 demultiplexer with long-term retention. The well-defined, controllable logic window and long-term retention of our new one-dimensional PLA element provide a promising route for building increasingly complex circuits with nanoscale building blocks

Evaluation of Electroencephalography Source Localization Algorithms with Multiple Cortical Sources

<div><p>Background</p><p>Source localization algorithms often show multiple active cortical areas as the source of electroencephalography (EEG). Yet, there is little data quantifying the accuracy of these results. In this paper, the performance of current source density source localization algorithms for the detection of multiple cortical sources of EEG data has been characterized.</p><p>Methods</p><p>EEG data were generated by simulating multiple cortical sources (2–4) with the same strength or two sources with relative strength ratios of 1:1 to 4:1, and adding noise. These data were used to reconstruct the cortical sources using current source density (CSD) algorithms: sLORETA, MNLS, and LORETA using a p-norm with p equal to 1, 1.5 and 2. Precision (percentage of the reconstructed activity corresponding to simulated activity) and Recall (percentage of the simulated sources reconstructed) of each of the CSD algorithms were calculated.</p><p>Results</p><p>While sLORETA has the best performance when only one source is present, when two or more sources are present LORETA with p equal to 1.5 performs better. When the relative strength of one of the sources is decreased, all algorithms have more difficulty reconstructing that source. However, LORETA 1.5 continues to outperform other algorithms. If only the strongest source is of interest sLORETA is recommended, while LORETA with p equal to 1.5 is recommended if two or more of the cortical sources are of interest. These results provide guidance for choosing a CSD algorithm to locate multiple cortical sources of EEG and for interpreting the results of these algorithms.</p></div

Genetic and Taqman qPCR assays used to identify <i>E. coli</i> mutants deficient in retrohoming.

<p>(A) Genetic assay. The Cam^R intron-donor plasmid pALG3 uses a T7lac promoter and phage Φ10 Shine-Dalgarno (SD) sequence to express an <i>ltrB</i>/GFP fusion cassette. This cassette consists of a 0.9-kb Ll.LtrB-ΔORF intron and flanking 5′- and 3′-exons (E1 and E2, respectively) <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003469#pgen.1003469-Guo1" target="_blank">[19]</a>, with the intron carrying a trimethoprim-resistance retrotransposition-activated genetic marker (Tp^R-RAM), and E2 linked in-frame to an ORF encoding GFP. The LtrA ORF preceded by its own Shine-Dalgarno sequence is co-transcribed from a position downstream of the GFP ORF. The Amp^R recipient plasmid contains a 45-bp Ll.LtrB target site (ligated E1–E2 sequence) upstream of a promoterless <i>tet^R</i> gene. T1 and T2 are <i>E. coli rrnB</i> transcription terminators, and TΦ is a phage T7 transcription terminator. (B) Taqman qPCR assay. The assay quantifies 5′- and 3′-intron integration junctions resulting from retrohoming of a retargeted Ll.LtrB-ΔORF intron into a site in the <i>rhlE</i> gene in the <i>E. coli</i> chromosome. Retrohoming events are quantified by Taqman qPCR, which utilizes the 5′→3′ exonuclease activity of Taq DNA polymerase to cleave a fluorescently labeled DNA probe that base pairs to an internal region of a PCR amplicon. Digestion of the probe by Taq DNA polymerase releases the FAM label (red star) free of the MGB quencher (green star), resulting in a quantifiable fluorescence signal for each amplification event. The numbers of 5′- and 3′-intron integration junctions relative to the number of <i>rhlE</i> targets were determined by quantifying the fluorescence signals in three separate PCRs relative to standard curves generated from serial dilutions of a reference plasmid (<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003469#s4" target="_blank">Materials and Methods</a>). Primers for these PCRs are depicted by arrows with numbers indicating the positions of the 5′ nucleotide of the upstream primer and 3′ nucleotide of the downstream primer relative to the intron-integration junction.</p