4 research outputs found
Small Surfactant Concentration Differences Influence Adsorption of Human Serum Albumin on Polystyrene Nanoparticles
Surfactants,
even in miniscule amounts, are often used for the
synthesis and especially the stabilization of nanomaterials, which
is essential for in vivo applications. In this study, we show that
the interaction between nanoparticles and proteins strongly depends
on the type of stabilizing surfactants and their (small) concentration
changes. The reaction between human serum albumin and polystyrene
nanoparticles stabilized by an ionic or nonionic surfactantsodium
dodecyl sulfate or Lutensol AT50, respectivelywas monitored
using isothermal titration calorimetry. It was found that the amount
of surfactant molecules on the surface significantly determines the
protein binding affinity and adsorption stoichiometry, which is important
for all nanomaterials coming into contact with biological components
such as blood plasma proteins. Thus after synthesizing nanomaterials
for in vivo applications as drug delivery agents, it is crucial to
perform a detailed analysis of the obtained surface chemistry that
accounts for the presence of minimal amounts of stabilizing agents
Denaturation via Surfactants Changes Composition of Protein Corona
The
use of nanocarriers as drug delivery vehicles brings them into
contact with blood plasma proteins. Polymeric nanocarriers require
some sort of surfactant to ensure colloidal stability. Formation of
the protein corona is therefore determined not only by the intrinsic
properties of the nanocarrier itself but also by the accompanying
surfactant. Although it is well-known that surfactants have an impact
on protein structure, only few studies were conducted on the specific
effect of surfactants on the composition of protein corona of nanocarriers.
Therefore, we analyzed the composition of the protein corona on “stealth”
nanoparticles with additional surfactant (cetyltrimethylammonium
chloride, CTMA-Cl) after plasma incubation. Additional CTMA-Cl led
to an enrichment of apolipoprotein-A1 and vitronectin in the corona,
while less clusterin could be found. Further, the structural stability
of apolipoprotein-A1 and clusterin was monitored for a wide range
of CTMA-Cl concentrations. Clusterin turned out to be more sensitive
to CTMA-Cl, with denaturation occurring at lower concentrations
Denaturation via Surfactants Changes Composition of Protein Corona
The
use of nanocarriers as drug delivery vehicles brings them into
contact with blood plasma proteins. Polymeric nanocarriers require
some sort of surfactant to ensure colloidal stability. Formation of
the protein corona is therefore determined not only by the intrinsic
properties of the nanocarrier itself but also by the accompanying
surfactant. Although it is well-known that surfactants have an impact
on protein structure, only few studies were conducted on the specific
effect of surfactants on the composition of protein corona of nanocarriers.
Therefore, we analyzed the composition of the protein corona on “stealth”
nanoparticles with additional surfactant (cetyltrimethylammonium
chloride, CTMA-Cl) after plasma incubation. Additional CTMA-Cl led
to an enrichment of apolipoprotein-A1 and vitronectin in the corona,
while less clusterin could be found. Further, the structural stability
of apolipoprotein-A1 and clusterin was monitored for a wide range
of CTMA-Cl concentrations. Clusterin turned out to be more sensitive
to CTMA-Cl, with denaturation occurring at lower concentrations
Combining Solar Steam Processing and Solar Distillation for Fully Off-Grid Production of Cellulosic Bioethanol
Conventional
bioethanol for transportation fuel typically consumes
agricultural feedstocks also suitable for human consumption and requires
large amounts of energy for conversion of feedstock to fuel. Alternative
feedstocks, optimally those not also in demand for human consumption,
and off-grid energy sources for processing would both contribute to
making bioethanol far more sustainable than current practices. Cellulosic
bioethanol production involves three steps: the extraction of sugars
from cellulosic feedstock, the fermentation of sugars to produce ethanol,
and the purification of ethanol through distillation. Traditional
production methods for extraction and distillation are energy intensive
and therefore costly, limiting the advancement of this approach. Here
we report an initial demonstration of the conversion of cellulosic
feedstock into ethanol by completely off-grid solar processing steps.
Our approach is based on nanoparticle-enabled solar steam generation,
in which high-efficiency steam can be produced by illuminating light-absorbing
nanoparticles dispersed in H<sub>2</sub>O with sunlight. We used solar-generated
steam to successfully hydrolyze feedstock into sugars; we then used
solar steam-distillation to purify ethanol in the final processing
step. Coastal hay, a grass grown for livestock feed across the southern
United States, and sugar cane as a control are successfully converted
to ethanol in this proof-of-concept study. This entirely off-grid
solar production method has the potential to realize the long-dreamed-of
goal of sustainable cellulosic bioethanol production