2 research outputs found
Fecundity and morphological description of the eggs of Psorophora cyanescens (Coquillett, 1902) (Diptera: Culicidae) by scanning electron microscopy
Taxonomic studies on mosquitoes are based on the morphological description of larvae and adults. However, few studies have focused on the morphological description of eggs despite their taxonomic value. The description of mosquito eggs by scanning electron microscopy (SEM) allows more detailed descriptions of the ornamentation of the egg, which can provide diagnostic characters of the species. The objective of the work was to describe the eggs of Psorophora cyanescens by SEM and provide fecundity data for the species. In general, Ps. cyanescens eggs are elliptical and very wide. The exochorion is characterized by polygon pattern, where each polygon consists of longitudinal ridges joined by cross-ridges defining regular "hexagonal" areas. Each longitudinal ridge consisting by a small tubercle. The small tubercles are irregular, rectangular, rounded, or tubular. The ornamentation of the exochorion also has long and conical tubercles throughout the egg region. The micropylar apparatus located in the anterior region of the egg has a prominent, continuous and thickness collar. Psorophora cyanescens laid mature eggs at 2.5 days post-feeding. The mean number of eggs per female was 82.20 (卤 13.31). This is the first study that describes the ultrastructure of the morphology of the eggs of Ps. cyanescens by SEM. With the contribution of the present work, there are five species of the genus Psorophora whose eggs morphology is described by SEM: Ps. albigenu, Ps. albipes, Ps. columbiae, Ps. cyanescens, and Ps. ferox.Los estudios taxon贸micos sobre mosquitos se basan en la descripci贸n morfol贸gica de larvas y adultos. Sin embargo, son pocos los estudios enfocados en la descripci贸n morfol贸gica de los huevos a pesar de su valor taxon贸mico. La descripci贸n de los huevos de mosquitos mediante microscop铆a electr贸nica de barrido (MEB) permite obtener im谩genes m谩s detalladas de la ornamentaci贸n del huevo, los cuales pueden proporcionar caracteres diagn贸sticos de la especie. El objetivo del trabajo fue describir los huevos de Psorophora cyanescens por MEB y proporcionar datos de fecundidad de la especie. En general, los huevos de Ps. cyanescens son de forma el铆ptica y muy anchos. El exocori贸n se caracteriza por un patr贸n de pol铆gono, donde cada pol铆gono consta de crestas longitudinales unidas por crestas cruzadas que definen 谩reas "hexagonales" regulares. Cada cresta longitudinal consiste en un peque帽o tub茅rculo. Los peque帽os tub茅rculos son irregulares, rectangulares, redondeados o tubulares. La ornamentaci贸n del exocori贸n tambi茅n presenta largos tub茅rculos c贸nicos en toda la regi贸n del huevo. El aparato micropilar ubicado en la regi贸n anterior del huevo tiene un collar prominente, continuo y grueso. Psorophora cyanescens puso huevos maduros a los 2.5 d铆as despu茅s de la alimentaci贸n sangu铆nea. El n煤mero promedio de huevos por hembra fue de 82.20 (卤 13.31). Este es el primer estudio que describe la ultraestructura de la morfolog铆a de los huevos de Ps. cyanescens mediante MEB. Con el aporte del presente trabajo, son cinco las especies del g茅nero Psorophora que cuentan con la descripci贸n de la morfolog铆a de los huevos mediante MEB: Ps. albigenu, Ps. albipes, Ps. columbiae, Ps. cyanescens, and Ps. ferox
Entomological and virological surveillance for dengue virus in churches in Merida, Mexico
ABSTRACT This study was designed to assess whether churches in endemic dengue districts in Merida, Mexico provide suitable breeding habitats for mosquitoes and are potential sites for dengue virus (DENV) transmission. Churches were inspected for immature and adult mosquitoes once every week from November 2015 to October 2016. A total of 10,997 immatures of five species were collected. The most abundant species were Aedes aegypti (6,051) and Culex quinquefasciatus (3,018). The most common source of immature Ae. aegypti were buckets followed by disposable containers. Adult collections yielded 21,226 mosquitoes of nine species. The most common species were Cx. quinquefasciatus (15,215) and Ae. aegypti (3,902). Aedes aegypti were found all year long. Female Ae. aegypti (1,380) were sorted into pools (166) and assayed for flavivirus RNA by RT-PCR and Sanger sequencing. Two pools were positive for DENV (DENV-1 and 2). In conclusion, we demonstrated that some churches in Merida are infested with mosquitoes all year long and they potentially serve as sites for DENV transmission and should therefore be considered for inclusion in mosquito and arboviruses control and surveillance efforts