Revealing the Complete Chloroplast Genome of an Andean Horticultural Crop, Sweet Cucumber (Solanum muricatum), and Its Comparison with Other Solanaceae Species

Sweet cucumber (Solanum muricatum) sect. Basarthrum is a neglected horticultural crop native of the Andean region. It is naturally distributed very close to other two Solanum crops of high importance, potatoes and tomatoes. To date, molecular tools for this crop are still undetermined. In this study, the complete sweet cucumber chloroplast (cp) genome was obtained and compared with seven Solanaceae species. The cp genome of S. muricatum had a 155,681 bp in length with included a large single copy (LSC) region of 86,182 bp and a small single-copy (SSC) region of 18,360 bp, separated by a pair of inverted repeats (IR) regions of 25,568 bp. The cp genome possessed 88 protein-coding genes (CDS), 37 transfer RNA (tRNA) genes, eight ribosomal RNA (rRNA) genes, and one pseudogene. Furthermore, 48 perfect microsatellites were identified, divided in mononucleotide repeats (32), followed by tetranucleotide (6) and dinucleotides (5). Microsatellites with trinucleotides repeats (3), pentanucleotide (1) and hexanucleotide (1) repeats motifs in these genomes were also identified, but in lower quantity. These repeats were mainly located in the noncoding regions. Whole cp genome comparative analysis revealed that the SSC and LSC regions showed more divergence than IR regions. Our phylogenetic analysis showed that S. muricatum is a sister species to members of sections Petota + Lycopersicum + Etuberosum. We expect to provide useful molecular data to shed light on the genetic diversity within sweet cucumber landrace, and also to determine the evolutionary processes in S. muricatum

Spironolactone Effect in Hepatic Ischemia/Reperfusion Injury in Wistar Rats

Introduction. Ischemia/reperfusion (IR) injury, often associated with liver surgery, is an unresolved problem in the clinical practice. Spironolactone is an antagonist of aldosterone that has shown benefits over IR injury in several tissues, but its effects in hepatic IR are unknown. Objective. To evaluate the effect of spironolactone on IR-induced damage in liver. Materials and Methods. Total hepatic ischemia was induced in rats for 20 min followed by 60 min of reperfusion. Spironolactone was administered and hepatic injury, cytokine production, and oxidative stress were assessed. Results. After IR, increased transaminases levels and widespread acute inflammatory infiltrate, disorganization of hepatic hemorrhage trabeculae, and presence of apoptotic bodies were observed. Administration of SPI reduced biochemical and histological parameters of liver injury. SPI treatment increased IL-6 levels when compared with IR group but did not modify either IL-1β or TNF-α with respect to IR group. Regarding oxidative stress, increased levels of catalase activity were recorded in IR + SPI group in comparison with group without treatment, whereas MDA levels were similar in IR + SPI and IR groups. Conclusions. Spironolactone reduced the liver damage induced by IR, and this was associated with an increase in IL-6 production and catalase activity

The Complete Mitochondrial Genome of a Neglected Breed, the Peruvian Creole Cattle (Bos taurus), and Its Phylogenetic Analysis

Cattle spread throughout the American continent during the colonization years, originating creole breeds that adapted to a wide range of climate conditions. The population of creole cattle in Peru is decreasing mainly due to the introduction of more productive breeds in recent years. During the last 15 years, there has been significant progress in cattle genomics. However, little is known about the genetics of the Peruvian creole cattle (PCC) despite its importance to (i) improving productivity in the Andean region, (ii) agricultural labor, and (iii) cultural traditions. In addition, the origin and phylogenetic relationship of the PCC are still unclear. In order to promote the conservation of the PCC, we sequenced the mitochondrial genome of a creole bull, which also possessed exceptional fighting skills and was employed for agricultural tasks, from the highlands of Arequipa for the first time. The total mitochondrial genome sequence is 16,339 bp in length with the base composition of 31.43% A, 28.64% T, 26.81% C, and 13.12% G. It contains 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes, and a control region. Among the 37 genes, 28 were positioned on the H-strand and 9 were positioned on the L-strand. The most frequently used codons were CUA (leucine), AUA (isoleucine), AUU (isoleucine), AUC (isoleucine), and ACA (threonine). Maximum likelihood reconstruction using complete mitochondrial genome sequences showed that the PCC is related to native African breeds. The annotated mitochondrial genome of PCC will serve as an important genetic data set for further breeding work and conservation strategies

Healthcare workers hospitalized due to COVID-19 have no higher risk of death than general population. Data from the Spanish SEMI-COVID-19 Registry

Aim To determine whether healthcare workers (HCW) hospitalized in Spain due to COVID-19 have a worse prognosis than non-healthcare workers (NHCW). Methods Observational cohort study based on the SEMI-COVID-19 Registry, a nationwide registry that collects sociodemographic, clinical, laboratory, and treatment data on patients hospitalised with COVID-19 in Spain. Patients aged 20-65 years were selected. A multivariate logistic regression model was performed to identify factors associated with mortality. Results As of 22 May 2020, 4393 patients were included, of whom 419 (9.5%) were HCW. Median (interquartile range) age of HCW was 52 (15) years and 62.4% were women. Prevalence of comorbidities and severe radiological findings upon admission were less frequent in HCW. There were no difference in need of respiratory support and admission to intensive care unit, but occurrence of sepsis and in-hospital mortality was lower in HCW (1.7% vs. 3.9%; p = 0.024 and 0.7% vs. 4.8%; p<0.001 respectively). Age, male sex and comorbidity, were independently associated with higher in-hospital mortality and healthcare working with lower mortality (OR 0.211, 95%CI 0.067-0.667, p = 0.008). 30-days survival was higher in HCW (0.968 vs. 0.851 p<0.001). Conclusions Hospitalized COVID-19 HCW had fewer comorbidities and a better prognosis than NHCW. Our results suggest that professional exposure to COVID-19 in HCW does not carry more clinical severity nor mortality

The complete chloroplast genome of the national tree of Peru, quina (Cinchona officinalis L., Rubiaceae)

Here, we report the first complete chloroplast (cp) genome of Cinchona officinalis. This cp genome has a 156,984 bp in length with typical quadripartite structure, containing a large single copy (LSC) region (83,929 bp) and an 18,051 bp small single-copy (SSC) region, separated by two inverted repeat (IR) regions (27,502 bp). The total GC content was 37.75%. Quina tree chloroplast genome possesses 135 genes that consisted of 89 protein-coding genes, 37 tRNA, eight rRNA, and one pseudogene. Phylogenetic analysis showed that C. officinalis is sister to C. pubescens and sister to them is Isertia laevis; all belong to the Cinchonoideae sub-family

Apocynin combined with drugs as coadjuvant could be employed to prevent and/or treat the chronic kidney disease

A worldwide public health problem is chronic kidney disease (CKD) presenting alarming epidemiological data. It currently affects about 10% of the adult population worldwide and has a high mortality rate. It is now known that oxidative stress represents one of the most important mechanisms in its pathophysiology, from the early stages to the terminal phase. Oxidation increases inflammation and reduces the capacity of NO• to relax vascular smooth muscle, in part by decreasing bioavailability of tetrahydrobiopterin (BH4), leading to endothelial dysfunction and high blood pressure, and due to the limited effectiveness of existing treatments, new drugs are needed to prevent and/or treat these mechanisms. The aim of this study was to test apocynin in a 5/6 nephrectomy mouse model of CKD to investigate whether its known antioxidant effect can improve the disease outcome. This effect results from the inhibition of NADPH oxidase and consequently a reduced production of the superoxide anion (). Animals were divided into five groups: sham, 5/6 nephrectomy only, and 5/6 nephrectomy followed by treatment with captopril, losartan or apocynin. The parameters evaluated were blood pressure and markers of oxidative stress () and endothelial function (BH4). There were significantly lower levels of and a greater availability of serum BH4 in the apocynin-treated animals versus the control group and the two other drug treatments. The present findings suggest that apocynin in conjunction with a coadjuvant for modulating blood pressure may be useful for controlling the progression of CRF

Campañas - PU122 - 202101

El curso integra todos los conocimientos y habilidades que el alumno ha adquirido en los ciclos anteriores y pretende simular el trabajo que se realiza en una agencia de publicidad considerando sus tres áreas principales: Planning, Creatividad y Medios, complementando este aprendizaje con el desarrollo de las competencias específicas de Estrategia Comunicacional, Creatividad y Ejecución Publicitaria que le permitirán diseñar y evaluar estrategias pertinentes de acuerdo a los objetivos establecidos. La comunicación publicitaria requiere de estrategias coherentes y consistentes a lo largo del tiempo, trabajadas de manera creativa y difundidas a través del mix adecuado de medios de comunicación

Campañas - PU122 - 202102

El curso integra todos los conocimientos y habilidades que el alumno ha adquirido en los ciclos anteriores y pretende simular el trabajo que se realiza en una agencia de publicidad considerando sus tres áreas principales: Planning, Creatividad y Medios, complementando este aprendizaje con el desarrollo de las competencias específicas de Estrategia Comunicacional, Creatividad y Ejecución Publicitaria que le permitirán diseñar y evaluar estrategias pertinentes de acuerdo a los objetivos establecidos. La comunicación publicitaria requiere de estrategias coherentes y consistentes a lo largo del tiempo, trabajadas de manera creativa y difundidas a través del mix adecuado de medios de comunicación

VII Jornadas de Intercambio de Experiencias Educativas : Avilés, 10, 11 y 12 de noviembre de 2008

Resumen basado en el de la publicaciónEn este CD-Rom encontraremos las ponencias presentadas en las VII Jornadas de Intercambio de Experiencias Educativas que se celebraron en el Centro del Profesorado y de Recursos de Avilés (Asturias). Las seis experiencias que se presentan, cuatro de Secundaria y dos de Primaria, son las siguientes: 1) El IES Virgen de la Luz (Avilés) centra su experiencia en la dinámica de funcionamiento de dos agrupaciones formativas inicialmente distintas, una centrada en las posibilidades educativas de las TIC y otra que aborda un taller de radio escolar, que valoran la oportunidad de colaborar y compartir trabajo, investigación e incluso puesta en práctica de actividades. 2) El IES Cristo del Socorro (Luanco) realiza una propuesta didáctica, canalizada a través del Departamento de Tecnología, orientada a facilitar el proceso de enseñanza-aprendizaje con carácter general en el aula y de manera especial ante situaciones de desfase curricular y problemas de aprendizaje. 3) La experiencia del IES Salinas (Salinas) constituye una muestra de algunas de las actividades realizadas en el 'Seminario de Integración de las TIC en el aula', desarrollado durante el curso escolar 2007-2008. 4) La propuesta del IES Juan Antonio Suanzes (Avilés) se corresponden con el trabajo realizado por los Departamentos de Física y Química e Inglés. Los materiales elaborados son de aplicación directa al aula y por tanto contemplan como objetivo principal servir de apoyo al aprendizaje y contribuir al avance en la competencia digital, de acuerdo con el curriculum LOE para la E.S.O y el Bachillerato. 5) 'De la carreta a la furgoletra', la experiencia del C.P. Virgilio Nieto (San Esteban de Pravia) repasa la evolución de la biblioteca del centro durante los últimos cinco años. El recorrido se inicia en 'carreta', luchando contra la precariedad de recursos, la desconfianza de las familias e incluso contra la intolerancia y la discriminación, hasta conseguir un Segundo Premio Nacional de Bibliotecas Escolares el año 2006, así como finalizar el curso 2007-08 poniendo en marca la furgoletra, que llevó al poblado donde viven los escolares, libros, ilusiones y esfuerzos compartidos. 6) Desarrollar conductas y actitudes viales seguras en los alumnos ha sido el objetivo que persigue el proyecto presentado por el C.P. Los Campos (Corvera de Asturias), una propuesta basada en la práctica diaria de la norma, frente a las habituales actuaciones de carácter puntual y esporádico, que pretende aprovechar las posibilidades educativas del juego en la infancia como instrumento para motivar y desarrollar hábitos y destrezas básicas, como base de comportamientos viales seguros. La experiencia ha merecido el reconocimiento de la D.G.T. que le ha concedido el Primer Premio Nacional en el 'IX Concurso de Proyectos de Educación Vial en el ámbito escolar' y más recientemente del Ministerio de Educación y del Ministerio del Interior conjuntamente, con el 'Primer Premio Nacional de Experiencias Pedagógicas sobre Seguridad en el entorno escolar'.AsturiasUniversidad de Oviedo. Facultad de Formación del Profesorado y Educación; Campus de Llamaquique, Calle Aniceto Sela, s. n.; 33005 Oviedo; +34985103215; +34985103214; [email protected]