Additional file 1: of Item analysis using Rasch models confirms that the Danish versions of the DISABKIDS® chronic-generic and diabetes-specific modules are valid and reliable

GLLRM's of the five subscales of DCGM Emotion, Social inclusion and exclusion, Physical limitation and treatment and the two subscales of DSM Impact and diabetes treatment. (DOCX 577 kb

Table_1_Transcriptome Analysis of Long Non-coding RNAs and Genes Encoding Paraspeckle Proteins During Human Ovarian Follicle Development.XLSX

<p>Emerging evidence indicated that many long non-coding (lnc)RNAs function in multiple biological processes and dysregulation of their expression can cause diseases. Most regulatory lncRNAs interact with biological macromolecules such as DNA, RNA, and protein. LncRNAs regulate gene expression through epigenetic modification, transcription, and posttranscription, through DNA methylation, histone modification, and chromatin remodeling. Interestingly, differential lncRNA expression profiles in human oocytes and cumulus cells was recently assessed, however, lncRNAs in human follicle development has not previously been described. In this study, transcriptome dynamics in human primordial, primary and small antral follicles were interrogated and revealed information of lncRNA genes. It is known that some lncRNAs form a complex with paraspeckle proteins and therefore, we extended our transcriptional analysis to include genes encoding paraspeckle proteins. Primordial, primary follicles and small antral follicles was isolated using laser capture micro-dissection from ovarian tissue donated by three women having ovarian tissue cryopreserved before chemotherapy. After RN sequencing, a bioinformatic class comparison was performed and primordial, primary and small antral follicles were found to express several lncRNA and genes encoding paraspeckle proteins. Of particular interest, we detected the lncRNAs XIST, NEAT1, NEAT2 (MALAT1), and GAS5. Moreover, we noted a high expression of FUS, TAF15, and EWS components of the paraspeckles, proteins that belong to the FET (previously TET) family of RNA-binding proteins and are implicated in central cellular processes such as regulation of gene expression, maintenance of genomic integrity, and mRNA/microRNA processing. We also interrogated the intra-ovarian localization of the FUS, TAF15, and EWS proteins using immunofluorescence. The presence and the dynamics of genes that encode lncRNA and paraspeckle proteins may suggest that these may mediate functions in the cyclic recruitment and differentiation of human follicles and could participate in biological processes known to be associated with lncRNAs and paraspeckle proteins, such as gene expression control, scaffold formation and epigenetic control through human follicle development. This comprehensive transcriptome analysis of lncRNAs and genes encoding paraspeckle proteins expressed in human follicles could potentially provide biomarkers of oocyte quality for the development of non-invasive tests to identify embryos with high developmental potential.</p

MOESM1 of Characterizing activity and assessing bycatch survival of Pacific halibut with accelerometer Pop-up Satellite Archival Tags

Additional file 1. Detailed description of plots used to assess Pacific halibut bycatch survival in Bering Sea trawl fisheries. File format:.pdf. Detailed analyses of PSAT data from healthy fish, carcass tags, and weighted tags in the Bering Sea along with additional details on procedures for inferring survival or mortality based on PSAT data

Ekologické vinohradnictví a vinařství na Mikulovsku

This thesis is focused on organic viticulture and winemaking in Mikulov region. This work evaluates development of organic viticulture in Mikulov viniculture region and description of organic grape growing and organic viticulture. This evaluation is focused on the areas of individual municipalities, the number and size of farms and the production and trade of organic wine. We pay special attention to the description of interspecific vine varieties, the cultivation areas and selected varieties. Thanks to our investigations of organic wine growers and organic farming winemakers we can characterize the situation in the sector, describe their motivation to farming in organic mode and prosperity of organic wine market. 5.3% of vineyards in the Mikulov region are in the organic mode. We asked 8 of 23 organic farmers in the region. All of them are constant in organic mode mainly because of the quality of its production

Additional file 2: of The cell surface mucin podocalyxin regulates collective breast tumor budding

Is Movie S1 showing control MCF-7 cells present little collective invasion 3-D culture, and Movie S2 showing podo-overexpressing cells send out very dynamic processes and show considerable collective invasion in 3-D culture. (ZIP 2262 kb