A SNP map of the European sea bass genome based on a meiotic gynogenetic family

International audienc

Absorbance spectrum of non-irradiated (control) egg extracts at pH8 (dotted line) and pH3 (solid line).

<p>Wavelengths cover almost the entire UV spectrum (UVC: 200–280 nm, UVB: 280–315 nm, and UVA: 315–400 nm). Absorption is shown as arbitrary units.</p

Representative examples of flow-cytometry histograms obtained from nuclear suspensions (5–10,000 counts) of Propidium Iodide (PI) stained larval samples.

<p>a) Control diploid (2n) larva (CV: 5%); b) haploid (n) larva produced with a UV-dose of 60 mJ.cm⁻² (CV: 10%). DNA values on the X-axis are reported in arbitrary units expressed as fluorescent channel numbers (PE-A). G2 represent mitotic peaks.</p

Morphology of control and UV-treated embryos and larvae.

<p>a) Control embryo at 74 h pf; b) control larva at hatching; c) UV treated embryos at 74 hpf showing microcephaly, short and large body; d) UV-treated larvae at hatching showing microphtalmy, short body and curved tail. Scale bars represent 500 µm.</p

Percent survival relative to controls of treated groups exposed to different cumulative UV-doses in the range 7.2–720 mJ.cm⁻².

<p>Error bars represent standard deviations of means (STD).</p

Percent survival relative to controls of hatched larvae issued from the different UV-irradiation treatments (7.2–72 mJ.cm⁻².min⁻¹) lasting 0.5–12 min.

<p>Error bars represent standard deviations of means (STD).</p

Microsatellite marker loci transmission in three putative androgenetic progenies (A1, B1 and C2) produced with a UV-dose of 60 mJ.cm⁻².

<p>Genotypes of progenies showing only discriminant paternal alleles are presented in bold characters. For putative androgenetic progenies, homozygous or haploid alleles are only written once since genotyping cannot distinguish between the presence of one or two copies of the same allele. N represents the number of analyzed individuals in each progeny.</p><p>Microsatellite marker loci transmission in three putative androgenetic progenies (A1, B1 and C2) produced with a UV-dose of 60 mJ.cm⁻².</p

Additional file 4: Table S4. of Gene-centromere mapping in meiotic gynogenetic European seabass

Position of microsatellites from previous studies and their informative level. (CSV 1 kb

Additional file 1: Table S1. of Gene-centromere mapping in meiotic gynogenetic European seabass

Detailed information for each sample used: Sample ID, origin, UV irradiation and shock parameters, sampling tissue, fertilisation, sampling date and barcodes used per sample. (CSV 19 kb