Nuclear accumulation of NFAT4 opposed by the JNK signal transduction pathway

The nuclear factor of activated T cells (NFAT) group of transcription factors is retained in the cytoplasm of quiescent cells. NFAT activation is mediated in part by induced nuclear import. This process requires calcium-dependent dephosphorylation of NFAT caused by the phosphatase calcineurin. The c-Jun amino-terminal kinase (JNK) phosphorylates NFAT4 on two sites. Mutational removal of the JNK phosphorylation sites caused constitutive nuclear localization of NFAT4. In contrast, JNK activation in calcineurin-stimulated cells caused nuclear exclusion of NFAT4. These findings show that the nuclear accumulation of NFAT4 promoted by calcineurin is opposed by the JNK signal transduction pathway

Genetic Evaluation of Children with Global Developmental Delay—Current Status of Network Systems in Taiwan

This review article aims to introduce the screening and referral network of genetic evaluation for children with developmental delay in Taiwan. For these children, integrated systems provide services from the medical, educational, and social welfare sectors. All cities and counties in Taiwan have established a network for screening, detection, referral, evaluation, and intervention services. Increased awareness improves early detection and intervention. There remains a gap between supply and demand, especially with regard to financial resources and professional manpower. Genetic etiology has a major role in prenatal causes of developmental delay. A summary of reports on some related genetic disorders in the Taiwanese population is included in this review. Genetic diagnosis allows counseling with regard to recurrence risk and prevention. Networking with neonatal screening, laboratory diagnosis, genetic counseling, and orphan drugs logistics systems can provide effective treatment for patients. In Taiwan, several laboratories provide genetic tests for clinical diagnosis. Accessibility to advanced expensive tests such as gene chips or whole exome sequencing is limited because of funding problems; however, the service system in Taiwan can still operate in a relatively cost-effective manner. This experience in Taiwan may serve as a reference for other countries

A Bibliometric Analysis on Dengue Outbreaks in Tropical and Sub-Tropical Climates Worldwide Since 1950

Severe dengue outbreaks (DOs) affect the majority of Asian and Latin American countries. Whether all DOs always occurred in sub-tropical and tropical areas (STTA) has not been verified. We downloaded abstracts by searching keywords “dengue (MeSH Major Topic)” from Pubmed Central since 1950, including three collections: country names in abstracts (CNA), no abstracts (WA), and no country names in abstracts (Non-CNA). Visualizations were created to present the DOs across countries/areas in STTA. The percentages of mentioned country names and authors’ countries in STTA were computed on the CNA and Non-CNA bases. The social network analysis was applied to highlight the most cited articles and countries. We found that (1) three collections are 3427 (25.48%), 3137 (23.33%), and 6884 (51.19%) in CNA, WA, and Non-CNA, respectively; (2) the percentages of 94.3% and 79.9% were found in the CNA and Non-CNA groups; (3) the most mentioned country in abstracts were India, Thailand, and Brazil; (4) most authors in the Non-CNA collections were from the United States, Brazil, and China; (5) the most cited article (PMID = 23563266) authored by Bhatt et al. had 2604 citations since 2013. Our findings provide in-depth insights into the DO knowledge. The research approaches are recommended for authors in research on other infectious diseases in the future, not just limited to the DO topic

The Bloodhound project: automating discovery of web usability issues using the InfoScent simulator

Usability Issues using the InfoScent ™ Simulator According to usability experts, the top user issue for Web sites is difficult navigation. We have been developing automated usability tools for several years, and here we describe a prototype service called InfoScent ™ Bloodhound Simulator, a push-button navigation analysis system, which automatically analyzes the information cues on a Web site to produce a usability report. We further build upon previous algorithms to create a method called Information Scent Absorption Rate, which measures the navigability of a site by computing the probability of users reaching the desired destinations on the site. Lastly, we present a user study involving 244 subjects over 1385 user sessions that shows how Bloodhound correlates with real users surfing for informatio