New record and distribution extension of Phalloceros spiloura Lucinda, 2008 (Cyprinodontiformes: Poeciliidae)

Phalloceros spiloura Lucinda, 2008 is known from the coastal drainages of Rio Grande do Sul and Santa Catarina States, Iguaçu and Uruguai river basins. Its geographic distribution is herein extended to a new basin, the Laguna dos Patos system, an isolated costal drainage from Southern Brazil

New record and distribution extension of Phalloceros spiloura Lucinda, 2008 (Cyprinodontiformes: Poeciliidae)

Phalloceros spiloura Lucinda, 2008 is known from the coastal drainages of Rio Grande do Sul and Santa Catarina States, Iguaçu and Uruguai river basins. Its geographic distribution is herein extended to a new basin, the Laguna dos Patos system, an isolated costal drainage from Southern Brazil

3-D Reconstruction of Rock Samples via Structure-From-Motion for Virtual Reality Applications: A Methodological Proposal

This article presents a methodological proposal for the three-dimensional reconstruction of rock samples via structure-from-motion. The presented methodological steps aimed to provide a reproducible workflow to create virtual rock samples to be applied in virtual applications. The proposed methodology works as a how-to guide as well as a preemptive troubleshooting guide for the complete process. Four geologists with different scholar levels volunteered to test this methodological proposal, applying it to three rock samples as the methodology steps were provided in an inverse-proportional manner to the graduate level. When analyzing the results of the performed reconstructions, all analyzed elements presented a proportional reduction due to the lack of information provided. An initial questionnaire was applied to verify the difficulties encountered, and subsequently, all volunteers received the complete methodology. In the second reconstruction, the results were equivalent to those obtained initially with the complete methodology. A technology acceptance model questionnaire was applied to determine the perception of utility and ease of use of the presented methodology. In both cases the results presented themselves in a positive way, indicating that the methodology was able to solve the problems found simply and objectively through a repeatable workflow

Sodium chloride levels for Colonial broiler chickens reared in free-range system

Objetivou-se determinar níveis de inclusão de cloreto de sódio (NaCl) em rações para aves machos e fêmeas da linhagem Colonial nas fases inicial (1 a 28 dias), de crescimento (28 a 56 dias) e final (56 a 84 dias) criadas em semiconfinamento. em cada ensaio, 480 aves com idade correspondente à fase de criação foram alojadas em 24 unidades experimentais contendo áreas de abrigo e de pastejo. O delineamento experimental utilizado foi o inteiramente casualizado, em esquema fatorial 4 × 2 (níveis de NaCl e sexos), totalizando oito tratamentos e três repetições de 20 aves. Os níveis de NaCl avaliados foram: 0,20; 0,40; 0,60 e 0,80% na fase inicial; 0,10; 0,30; 0,50 e 0,70% na fase de crescimento e 0,10; 0,25; 0,40 e 0,55% na fase final. Foram avaliados o ganho de peso, o consumo de ração, a ingestão de água, a conversão alimentar, o potencial hidrogeniônico, a pressão parcial de gás carbônico no sangue, a concentração de hematócritos e os teores de sódio e cloro no sangue. Para a fase inicial, o nível recomendado é de 0,53% de NaCl na ração para aves de ambos os sexos. Para a fase de crescimento, os níveis de 0,40 e 0,43% de NaCl, respectivamente, para machos e fêmeas e, para a fase final, de 0,25% para aves de ambos os sexos, atendem às exigências nutricionais.The objective of this work was to determine inclusion levels of sodium chloride (NaCl) in diets for male and female Colonial strain birds in the starter (from one to 28 days of age), growing (from 28 to 56 days of age) and finisher (from 56 to 84 days of age) phases reared in free-range system. In each trial, 480 birds at age corresponding to the rearing phase were housed in 24 experimental units containing areas of shelter and pasture. The experimental design used was a completely randomized in a 4 × 2 factorial arrangement (NaCl levels × sex), totaling eight treatments and three replications of 20 birds. Levels of NaCl evaluated were: 0.20, 0.40, 0.60 and 0.80% in the starter phase; 0.10, 0.30, 0.50 and 0.70% in the growing phase and 0.10, 0.25, 0.40 and 0.55% in the finisher phase. It was evaluated weight gain, feed intake, water intake, feed conversion, pH, partial pressure of carbon dioxide in the blood, hematocrit concentration and sodium and chlorine blood levels. For the starter phase it is recommended 0.53% of NaCl level in diet for birds of both sexes. For the growing phase, the levels were 0.40 and 0.43% of NaCl, respectively, for males and females, and for the finisher phase, the level of 0.25% for birds of both sexes meets the nutritional requirements

The citrus flavonoid naringenin impairs the in vitro infection of human cells by Zika virus

Submitted by Manoel Barata ([email protected]) on 2019-12-03T19:15:53Z No. of bitstreams: 1 s41598-019-52626-3ok.pdf: 5313799 bytes, checksum: d20c9fb2c504897a712c12579e70ca58 (MD5)Approved for entry into archive by Manoel Barata ([email protected]) on 2019-12-18T20:05:03Z (GMT) No. of bitstreams: 1 s41598-019-52626-3ok.pdf: 5313799 bytes, checksum: d20c9fb2c504897a712c12579e70ca58 (MD5)Made available in DSpace on 2019-12-18T20:05:03Z (GMT). No. of bitstreams: 1 s41598-019-52626-3ok.pdf: 5313799 bytes, checksum: d20c9fb2c504897a712c12579e70ca58 (MD5) Previous issue date: 2019Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas. Departamento de Física e Química. Laboratório de Física Biológica. Ribeirão Preto, São Paulo, Brasil.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas. Laboratório de Glicoimunologia. Ribeirão Preto, São Paulo, Brasil.Universidade Estadual de Londrina. Centro de Ciências Biológicas. Departamento de Ciências Patológicas. Londrina, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Células Tronco. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Células Tronco. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.The Zika virus (ZIKV) is an arthropod-borne virus that belongs to the Flaviviridae family. The ZIKV infection is usually asymptomatic or is associated with mild clinical manifestations; however, increased numbers of cases of microcephaly and birth defects have been recently reported. To date, neither a vaccine nor an antiviral treatment has become available to control ZIKV replication. Among the natural compounds recognized for their medical properties, flavonoids, which can be found in fruits and vegetables, have been found to possess biological activity against a variety of viruses. Here, we demonstrate that the citrus flavanone naringenin (NAR) prevented ZIKV infection in human A549 cells in a concentration-dependent and ZIKV-lineage independent manner. NAR antiviral activity was also observed when primary human monocyte-derived dendritic cells were infected by ZIKV. NAR displayed its antiviral activity when the cells were treated after infection, suggesting that NAR acts on the viral replication or assembly of viral particles. Moreover, a molecular docking analysis suggests a potential interaction between NAR and the protease domain of the NS2B-NS3 protein of ZIKV which could explain the anti-ZIKV activity of NAR. Finally, the results support the potential of NAR as a suitable candidate molecule for developing anti-ZIKV treatments