Anthropogenically altered runoff processes in a waterlogged headwater catchment within the National Park Hunsrück-Hochwald, Germany

This study is concerned with an initial investigation of anthropogenically altered runoff processes in a headwater catchment within the National Park Hunsrück-Hochwald (Germany) that is characterized by slope bogs and waterlogged soils. The examined area is crossed by a dense network of trenches, which were established in the course of forestry operations in order to utilize these waterlogged areas. An evaluation of the drainage network’s influence on runoff processes is attempted using water gauges and GIS-based analyses of the subcatchments. Results of the water year 2016 and a heavy rainfall event show that gauges in the study area react quickly to precipitation inputs and that water is retained only for a short time. The magnitude of runoff recession even in short dry spells permits the conclusion that the already merely residual slope bogs are endangered. The partial results of this study can serve as an instrument for rewetting actions as they allow spatially and temporally high resolved statements about the influence of drainage networks. Furthermore, this study is embedded in a long-term monitoring of hydrological processes and represents a first component of a detailed process-measurement taking place

Surface runoff of horse grazed pasture – a disregarded hydrological response unit in low mountain ranges

Accurate prediction of surface runoff is of vital interest for flood prediction which in turn requires the process knowledge about key factors affecting its temporal and spatial variability. Antecedent soil moisture and grazing intensity have been detected as important factors, but there exists no explicit field study investigating the spatial and temporal variability of surface runoff generation on horse grazed pasture. In our study, for the first time the surface runoff generation on horse grazed pasture was analyzed using a rainfall simulator along with measurements of soil water content and soil physical properties. The results were compared with concurrent investigations on cattle grazed pasture land. The analyses of 8 rainfall simulations on 1 m² plots at a rate of 46.6 mm/h revealed mean runoff coefficients ranging from 0.9% to 50.5%. The most important findings of our study are that the antecedent soil moisture distinctly impacts the amount of surface runoff and the runoff coefficient is significantly higher on horse grazed pasture than on cattle grazed pasture. These results underline the importance of further experimental studies to obtain a broader process knowledge about this specific hydrological response unit, especially in regard to the increasing portion of horse grazing in the low mountain ranges

Factores que influyen en la dispersión espacio-temporal de broca del café Hypothenemus hampei (Coleoptera: Curculionidae)

La broca del café, Hypothenemus hampei (Ferrari), es la plaga más importante del café, Coffea spp., en el mundo. Este insecto ha sido motivo de numerosos estudios, sin embargo, muchos aspectos de su dispersión se desconocen o requieren ser determinados. La presente investigación tuvo el objetivo de estudiar los factores que influyen en la dispersión espacio-temporal de la broca del café. Dado que la dispersión es un proceso complejo, para ser estudiada se desarrollaron técnicas para la obtención del material biológico con condiciones adecuadas para el estudio, para facilitar el estudio de la morfología del complejo espermático de H. hampei y de marcaje-liberación-recaptura para estudiar la capacidad de vuelo y los factores que la afectan. Los resultados más sobresalientes indican que la espermateca de H. hampei está ubicada en la parte final del abdomen entre la glándula accesoria y el oviducto común y está compuesta por un ducto espermático, músculos espermáticos y una glándula espermática; además, posee músculos al final de su curvatura extrema y su cutícula está finamente reticulada. Los espermatozoides se observaron como filamentos minúsculos, endebles y homogéneos, congregados longitudinalmente dentro de la espermateca. La emergencia masiva de las CH se relacionó con las lluvias y se presentó entre febrero y junio, que corresponde al periodo intercosecha. Todas las CH examinadas tenían espermatozoides en la espermateca, lo q ue sugiere que se habían apareado antes de abandonar el fruto natal. La sobrevivencia de las hembras colonizadoras fue mayor en ambientes humedos y en sustratos diferentes a su hospedero. Durante el periodo de fructificación del café, las hembras se dispersaron mediante vuelo a los glomérulos de frutos adyacentes. Después de colonizar un fruto, la CH perdió su capacidad de vuelo de forma gradual en el transcurso de 5 a 6 días; durante este tiempo, las hembras evaluadas realizar más de un vuelo. Algunas CH marcadas y liberadas se recapturaron a 75 m de distancia del sitio de liberación a las 24 h después de haber sido liberadas. Finalmente, se discute la importancia de los hallazgos de la dispersión espacio-temporal de la broca del café, como información clave para mejorar la comprensión del complejo café-broca, y el manejo de la broca. (Résumé d'auteur

The MalR type regulator AcrC is a transcriptional repressor of acarbose biosynthetic genes in Actinoplanes sp. SE50/110

Wolf T, Droste J, Gren T, et al. The MalR type regulator AcrC is a transcriptional repressor of acarbose biosynthetic genes in Actinoplanes sp. SE50/110. BMC Genomics. 2017;18(1): 562.Background Acarbose is used in the treatment of diabetes mellitus type II and is produced by Actinoplanes sp. SE50/110. Although the biosynthesis of acarbose has been intensively studied, profound knowledge about transcription factors involved in acarbose biosynthesis and their binding sites has been missing until now. In contrast to acarbose biosynthetic gene clusters in Streptomyces spp., the corresponding gene cluster of Actinoplanes sp. SE50/110 lacks genes for transcriptional regulators. Results The acarbose regulator C (AcrC) was identified through an in silico approach by aligning the LacI family regulators of acarbose biosynthetic gene clusters in Streptomyces spp. with the Actinoplanes sp. SE50/110 genome. The gene for acrC, located in a head-to-head arrangement with the maltose/maltodextrin ABC transporter malEFG operon, was deleted by introducing PCR targeting for Actinoplanes sp. SE50/110. Characterization was carried out through cultivation experiments, genome-wide microarray hybridizations, and RT-qPCR as well as electrophoretic mobility shift assays for the elucidation of binding motifs. The results show that AcrC binds to the intergenic region between acbE and acbD in Actinoplanes sp. SE50/110 and acts as a transcriptional repressor on these genes. The transcriptomic profile of the wild type was reconstituted through a complementation of the deleted acrC gene. Additionally, regulatory sequence motifs for the binding of AcrC were identified in the intergenic region of acbE and acbD. It was shown that AcrC expression influences acarbose formation in the early growth phase. Interestingly, AcrC does not regulate the malEFG operon. Conclusions This study characterizes the first known transcription factor of the acarbose biosynthetic gene cluster in Actinoplanes sp. SE50/110. It therefore represents an important step for understanding the regulatory network of this organism. Based on this work, rational strain design for improving the biotechnological production of acarbose can now be implemented

Proton Magnetic Resonance Spectroscopy Reveals Neuroprotection by Oral Minocycline in a Nonhuman Primate Model of Accelerated NeuroAIDS

Background: Despite the advent of highly active anti-retroviral therapy (HAART), HIV-associated neurocognitive disorders continue to be a significant problem. In efforts to understand and alleviate neurocognitive deficits associated with HIV, we used an accelerated simian immunodeficiency virus (SIV) macaque model of NeuroAIDS to test whether minocycline is neuroprotective against lentiviral-induced neuronal injury. Methodology/Principal Findings: Eleven rhesus macaques were infected with SIV, depleted of CD8+ lymphocytes, and studied until eight weeks post inoculation (wpi). Seven animals received daily minocycline orally beginning at 4 wpi. Neuronal integrity was monitored in vivo by proton magnetic resonance spectroscopy and post-mortem by immunohistochemistry for synaptophysin (SYN), microtubule-associated protein 2 (MAP2), and neuronal counts. Astrogliosis and microglial activation were quantified by measuring glial fibrillary acidic protein (GFAP) and ionized calcium binding adaptor molecule 1 (IBA-1), respectively. SIV infection followed by CD8+ cell depletion induced a progressive decline in neuronal integrity evidenced by declining N-acetylaspartate/creatine (NAA/Cr), which was arrested with minocycline treatment. The recovery of this ratio was due to increases in NAA, indicating neuronal recovery, and decreases in Cr, likely reflecting downregulation of glial cell activation. SYN, MAP2, and neuronal counts were found to be higher in minocycline-treated animals compared to untreated animals while GFAP and IBA-1 expression were decreased compared to controls. CSF and plasma viral loads were lower in MN-treated animals. Conclusions/Significance: In conclusion, oral minocycline alleviates neuronal damage induced by the AIDS virus

Runoff and Soil Erosion Assessment on Forest Roads Using a Small Scale Rainfall Simulator

Forestry operations can significantly alter hydrological and erosional processes in a catchment. In the course of developing timberland, a network of persistent roads and skid trails causing soil compaction is usually established. Hereby, the infiltration rate of the soil is distinctly reduced, which leads to the generation of overland flow—this may also cause soil erosion. In this study, a small-scale rainfall simulator is used to investigate hydrological and erosional processes on forest roads and skid trails. The results show increased runoff rates on forest roads, up to 25 times higher than on undisturbed forest topsoil. On skid trails, the runoff rates were altered especially in rutted areas (16 times higher) while unrutted parts showed a lesser change (four times higher). With sufficient overland flow, soil erosion rates also rose, particularly when the vegetation cover of the surface was removed: bare road surfaces featured higher mean erosion rates (195 g·m−2) than partly or completely vegetated skid trails (13 g·m−2) and undisturbed sites (5 g·m−2). The findings presented in this study indicate the need for the use of compaction reducing technology during forestry operations and a revegetation of road surfaces in order to minimize the detrimental factor of roads and skid trails on water retention and soil conservation

Modeling Runoff-Formation and Soil Erosion after Pumice Excavation at Forested Andosol-Sites in SW-Germany Using WEPP

This study investigates the effects of pumice excavation on runoff formation and soil erosion processes in a forested catchment in SW-Germany. The underlying questions are, if (a) backfilled soils have different properties concerning runoff generation and erodibility and if (b) clear-cutting prior to excavation triggers runoff and erosion. Four adjacent sub-areas were observed, which represented different pre- and post-excavation-stages. The basis of the investigation was a comprehensive field sampling that delivered the data for physical erosion modeling using the Water Erosion Prediction Project (WEPP). Modeling took place for standardized conditions (uniform slope geometry and/or uniform land management) and for actual slope geometry and land management. The results show that backfilled soils exhibited 53% increase of annual runoff and 70% increase of annual soil loss under standardized conditions. Storm runoff was increased by 6%, while storm soil loss was reduced by 9%. Land management changes also triggered shifts in annual runoff and soil erosion: Clear-cut (+1.796% runoff, +4.205% soil loss) and bare (+5.958% runoff, +21.055% soil loss) surfaces showed the most distinct changes when compared to undisturbed forest. While reforestation largely diminished post-excavation runoff and soil erosion, the standardized results statistically prove that soil erodibility and runoff generation remain increased after backfilling

Influence of inclination and permeability of solitary woody riparian plants on local hydraulic and sedimentary processes

International audienceThe role of solitary woody riparian plants with respect to local erosion and deposition of sediments is investigated. A focus is laid on the characteristics ‘inclination’ and ‘permeability’ of the plant's projected frontal area. Therefore, two experimental studies using cylindrical obstacles were carried out in a laboratory flume, one aiming at inclination, the other at permeability. The first series revealed that the total amount of mobilized sediment around the cylinder on average decreased by 8–10% per 5° increasing inclination in streamwise direction. Locations of maximum scour depth simultaneously shifted downstream. A horseshoe vortex system, causing the frontal and lateral scouring, ceased to exist below inclinations of 25–30°. The second series revealed that with increasing permeability, frontal scour incision is delayed, and the eroded sediment volume is significantly reduced. With permeable obstacles, two system states were observed: first, frontal scouring with leeside deposition at higher flow velocities and, second, moderate leeside scouring at lower flow velocities. For up‐scaling and comparison, a field study focussing on fluvial obstacle marks at poplars and willows in secondary channels of the River Loire was additionally conducted. A modified analytical model enabled us to quantify the amount of deposited sediments leeside of the plants. Leeside sediment ridges are significantly stabilized and have a higher preservation potential when covered by pioneer vegetation. Under such conditions, they may indeed induce the development of stable islands. Eventually, ‘sediment ridge width’ turned out to be a suitable indicator for leeside deposited sediment volume, irrespective of spatial scale

The Influence of Soil Compaction on Runoff Formation. A Case Study Focusing on Skid Trails at Forested Andosol Sites

This study discusses the influence of soil compaction on runoff generation with a special focus on forested Andosol sites. Because of their typical soil physical characteristics (low bulk density, high pore volumes) and the existent land use, these areas are expected to show low to no measurable overland flow during heavy rainfall events. However, due to heavy machinery traffic in the course of forestry actions and pumice excavations, skid trails have been established. Here, a distinct shift of soil dry bulk density (DBD) was observable, using a detailed soil mapping and data interpolation in order to generate in-depth DBD-cross profiles. Additionally, infiltration measurements and rainfall simulations (I = 45 mm·h−1, t = 30 min) were conducted to evaluate effects of observed soil compaction on infiltration rates and overland flow formation. Results show that soil compaction was increased by 21% on average in skid trail wheel ruts. As a consequence, observed runoff was 8.5-times higher on skid trails, while saturated hydraulic conductivity was diminished by 36%. These findings show, that soil compaction leads to a higher possibility of runoff formation during heavy rainfall events, especially at sites which showed initial conditions with presumably low tendencies of runoff formation