9 research outputs found
Circadian Desynchrony Promotes Metabolic Disruption in a Mouse Model of Shiftwork
Shiftwork is associated with adverse metabolic pathophysiology, and the rising incidence of shiftwork in modern societies is thought to contribute to the worldwide increase in obesity and metabolic syndrome. The underlying mechanisms are largely unknown, but may involve direct physiological effects of nocturnal light exposure, or indirect consequences of perturbed endogenous circadian clocks. This study employs a two-week paradigm in mice to model the early molecular and physiological effects of shiftwork. Two weeks of timed sleep restriction has moderate effects on diurnal activity patterns, feeding behavior, and clock gene regulation in the circadian pacemaker of the suprachiasmatic nucleus. In contrast, microarray analyses reveal global disruption of diurnal liver transcriptome rhythms, enriched for pathways involved in glucose and lipid metabolism and correlating with first indications of altered metabolism. Although altered food timing itself is not sufficient to provoke these effects, stabilizing peripheral clocks by timed food access can restore molecular rhythms and metabolic function under sleep restriction conditions. This study suggests that peripheral circadian desynchrony marks an early event in the metabolic disruption associated with chronic shiftwork. Thus, strengthening the peripheral circadian system by minimizing food intake during night shifts may counteract the adverse physiological consequences frequently observed in human shift workers
Tissue-specific dissociation of diurnal transcriptome rhythms during sleep restriction in mice
Study objectives: Shortened or mistimed sleep affects metabolic homeostasis, which may in part be mediated by dysregulation of endogenous circadian clocks. In this study, we assessed the contribution of sleep disruption to metabolic dysregulation by analysing diurnal transcriptome regulation in metabolic tissues of mice subjected to a sleep restriction (SR) paradigm. Methods: Male mice were subjected to 2 × 5 days of SR with enforced waking during the first 6 hours of the light phase. SR and control mice were sacrificed at different time points of the day and RNA preparations from the mediobasal hypothalamus (MBH), liver, and epididymal white adipose tissue (eWAT) were subjected to whole-genome microarray hybridization. Transcriptional rhythms were associated with changes in behavioral and physiological parameters such as sleep, body temperature, and food intake. Rhythm detection was performed with CircWave and transcription profiles were compared by 2-way analysis of variance and t-tests with Benjamini-Hochberg corrections. Results: Clock gene rhythms were blunted in all tissues, while transcriptome regulation was associated with either clock gene expression, sleep patterns, or food intake in a tissue-specific manner. Clock gene expression was associated with apoptosis pathways in the MBH and with tumor necrosis factor alpha signalling in liver. Food intake-associated genes included cilium movement genes in the MBH and lipid metabolism-associated transcripts in liver. Conclusions: In mice, repeated SR profoundly alters behavioral and molecular diurnal rhythms, disrupting essential signalling pathways in MBH, liver, and eWAT, which may underlie the metabolic and cognitive disturbances observed in sleep-restricted humans such as shift workers
Hepatic gene therapy rescues high-fat diet responses in circadian Clock mutant mice
Objective Circadian Clock gene mutant mice show dampened 24-h feeding rhythms and an increased sensitivity to high-fat diet (HFD) feeding. Restricting HFD access to the dark phase counteracts its obesogenic effect in wild-type mice. The extent to which altered feeding rhythms are causative for the obesogenic phenotype of Clock mutant mice, however, remains unknown. Methods Metabolic parameters of wild-type (WT) and Clock mutant mice (MT) were investigated under ad libitum and nighttime restricted HFD feeding. Liver circadian clock function was partially rescued by hydrodynamic tail vein delivery of WT-Clock DNA vectors in mutant mice and transcriptional, metabolic, endocrine and behavioral rhythms studied. Results Nighttime-restricted feeding restored food intake, but not body weight regulation in MT mice under HFD, suggesting Clock-dependent metabolic dysregulation downstream of circadian appetite control. Liver-directed Clock gene therapy partially restored liver circadian oscillator function and transcriptome regulation without affecting centrally controlled circadian behaviors. Under HFD, MT mice with partially restored liver clock function (MT-LR) showed normalized body weight gain, rescued 24-h food intake rhythms, and WT-like energy expenditure. This was associated with decreased nighttime leptin and daytime ghrelin levels, reduced hepatic lipid accumulation, and improved glucose tolerance. Transcriptome analysis revealed that hepatic Clock rescue in MT mice affected a range of metabolic pathways. Conclusion Liver Clock gene therapy improves resistance against HFD-induced metabolic impairments in mice with circadian clock disruption. Restoring or stabilizing liver clock function might be a promising target for therapeutic interventions in obesity and metabolic disorders
Metabolic effects of TSR.
<p>(A) Pyruvate tolerance test in control and TSR mice (n = 5). Circulating glycerol (B) and triglyceride (C) rhythms in control and TRS mice (n = 3 at each time point). (D) Liver glycogen levels in control and TSR mice (n = 3 at each time point) (E) Leptin levels during light and dark phase in control and TRS mice (n = 10). Control mice are represented by black lines, TSR mice by grey lines; * indicates p<0.05 and **p<0.01 determined by 2-way ANOVA.</p
TSR resets the hepatic circadian clock.
<p>(A) qPCR analysis of diurnal clock gene mRNA profiles in the liver of control and TSR mice (n = 3 at each time point). (B) Luciferase activity in liver slice cultures from control and TSR <i>PER2::LUC</i> mice (n = 8) and (C) quantitation of phase and (D) period. Control mice are represented by black lines, TSR mice by grey lines; * indicates p<0.05 determined by 2-way ANOVA.</p
TSR results in perturbations of diurnal behavioral rhythms.
<p>(A and B) Representative activity recordings of TSR mice. Actograms are double-plotted. Grey shadings indicate dark phases, red boxes indicate period of TSR. (C) Plasma corticosterone in control and TSR mice on Day12 of TSR (n = 3−5). (D) Distribution of light and dark activity during TSR expressed as % of total activity of the same set of animals during control conditions (n = 5 cages of 4 mice each), * indicates p<0.05 and **p<0.01 relative to 0 weeks of TSR, § indicates p<0.05 relative to 1 week of TSR. (E) Food intake during light and dark phase during TSR, * indicates p<0.05 and **p<0.01 relative to 0 weeks of TSR (n = 10−23), § indicates p<0.05 relative to 1 week of TSR determined by 2-way ANOVA. (F) Quantitation of clock gene mRNA by ISH in the SCN of control and TSR mice expressed relative to controls at ZT0 (n = 3 at each time point). (G) Activity of control and TSR mice upon release into DD (n = 3 cages). Grey bars indicate dark phase, red box indicates last period of gentle handling. Control mice represented by black lines, TSR mice by grey lines; * indicates p<0.05 determined by 2-way ANOVA.</p
TSR causes global disruption of transcriptome rhythmicity in the liver.
<p>(A) Heat map illustrating diurnal expression of rhythmically expressed genes sorted for phase in control animals, and their corresponding expression profiles in TSR animals in liver (n = 3 at each time point). (B) Summary of transcriptional effects seen in liver following TSR. (C) Peak times of rhythmically expressed genes under control and TSR conditions. Black bars and lines indicate controls, grey bars and red lines indicate TSR.</p
Dark phase feeding rescues TSR-induced disruption of peripheral clock gene expression and gluconeogenesis.
<p>(A) qPCR analysis of clock gene expression in the liver of control/dark phase fed (DF) and TSR/dark phase fed (DF-TSR) mice. (B) Plasma glycerol and (C) triglyceride rhythms in control/DF and DF-TSR mice. (D) Pyruvate tolerance test in control/DF and DF-TSR mice (n = 5). (E) Plasma corticosterone control/DF fed and DF-TSR mice. (F) Pyruvate tolerance test in non-TRS mice under a TSR-like feeding regimen (n = 5). (A-E) Control mice are represented by black lines, TSR mice by grey lines. (F) <i>Ad libitum</i> fed mice are represented by black lines, TSR-like fed mice are represented by grey lines; * indicates p<0.05 determined by 2-way ANOVA, n = 3 at each time point, unless stated otherwise.</p
Metabolic gene rhythmicity in the liver is affected by shiftwork.
<p>Double plotted expression profiles of rhythmic genes associated with the GO terms ‘Carbohydrate Metabolism’, ‘Lipid Metabolism’ and ‘Amino Acid Metabolism’ sorted for peak time ((A) ZT1, (B) ZT7, (C) ZT13 and (D) ZT19) for both control and TSR groups. (E) Array expression profiles of selected metabolically relevant genes with altered expression profiles in the livers of TSR mice. Control mice are represented by black lines, TSR mice by grey lines; * indicates p<0.05 determined by 2-way ANOVA.</p