Validation of a coupled <em>δ</em>²H_{<em>n</em>-alkane}–<em>δ</em>¹⁸O_sugarpaleohygrometer approach based on a climate chamber experiment.

The hydrogen isotope composition of leaf-wax-derived biomarkers, e.g., long-chain n-alkanes (delta H-2(n-alkane)), is widely applied in paleoclimate. However, a direct reconstruction of the isotope composition of source water based on delta H-2(n-alkane) alone is challenging due to the enrichment of heavy isotopes during evaporation. The coupling of delta H-2(n-alkane) with delta O-18 of hemicellulose-derived sugars (delta O-18(sugar)) has the potential to disentangle this limitation and additionally to allow relative humidity reconstructions. Here, we present delta H-2(n-alkane) as well as delta O-18(sugar) results obtained from leaves of Eucalyptus globulus, Vicia faba, and Brassica oleracea, which grew under controlled conditions. We addressed the questions of (i) whether delta H-2(n-alkane) and delta O-18(sugar) values allow reconstructions of leaf water isotope composition, (ii) how accurately the reconstructed leaf water isotope composition enables relative humidity (RH) reconstruction, and (iii) whether the coupling of delta H-2(n-alkane) and delta O-18(sugar) enables a robust source water calculation. For all investigated species, the n-alkane n-C-29 was most abundant and therefore used for compound-specific delta H-2 measurements. For Vicia faba, additionally the delta H-2 values of n-C-31 could be evaluated robustly. Regarding hemicellulose-derived monosaccharides, arabinose and xylose were most abundant, and their delta O-18 values were therefore used to calculate weighted mean leaf delta O-18(sugar) values.Both delta H-2(n-alkane) and delta O-18(sugar) yielded significant correlations with delta H-2(leaf water) and delta O-18(leaf water), respectively (r(2)=0.45 and 0.85, respectively; p&lt;0.001, n=24). Mean fractionation factors between biomarkers and leaf water were found to be -156 parts per thousand (ranging from -133 parts per thousand to -192 parts per thousand) for epsilon(n-alkane/leaf water) and +27.3 parts per thousand (ranging from +23.0 parts per thousand to 32.3 parts per thousand) for epsilon(sugar/leaf water), respectively. Modeled RHair values from a Craig-Gordon model using measured Tair, delta 2Hleaf water and delta 18Oleaf water as input correlate highly significantly with modeled RHair values (R2=0.84, p&lt;0.001, RMSE = 6 %). When coupling delta H-2(n-alkane) and delta O-18(sugar) values, the correlation of modeled RHair values with measured RHair values is weaker but still highly significant, with R-2=0.54 (p&lt;0.001, RMSE = 10 %). Finally, the reconstructed source water isotope composition (delta H-2(s) and delta O-18(s)) as calculated from our coupled approach matches the source water in the climate chamber experiment (delta H-2(tank water) and delta O-18(tank water)). This highlights the great potential of the coupled delta H-2(n-alkane)-delta O-18(sugar) paleohygrometer approach for paleoclimate and relative humidity reconstructions

Environmental effects of anticholinesterasic therapeutic drugs on a crustacean species, Daphnia magna

The presence of pharmaceutical drugs in the environment is an important field of toxicology, since such residues can cause deleterious effects on exposed biota. This study assessed the ecotoxicological acute and chronic effects of two anticholinesterasic drugs, neostigmine and pyridostigmine in Daphnia magna. Our study calculated 48 h-EC50 values for the immobilization assay of 167.7 μg L(-1) for neostigmine and 91.3 μg L(-1) for pyridostigmine. In terms of feeding behavior, we calculated a 5 h-EC50 for filtration rates of 7.1 and 0.2 μg L(-1) for neostigmine and pyridostigmine, respectively; for the ingestion rates, the calculated EC50 values were, respectively, 7.5 and 0.2 μg L(-1) for neostigmine and pyridostigmine. In the reproduction assay, the most affected parameter was the somatic growth rate (LOECs of 21.0 and 2.9 μg L(-1) for neostigmine and pyridostigmine, respectively), followed by the fecundity (LOECs of 41.9 and 11.4 μg L(-1) for neostigmine and pyridostigmine, respectively). We also determined a 48 h-IC50 for cholinesterase activity of 1.7 and 4.5 μg L(-1) for neostigmine and pyridostigmine, respectively. These results demonstrated that both compounds are potentially toxic for D. magna at concentrations in the order of the μg L(-1)