19 research outputs found
Chloroplast localization of Cry1Ac and Cry2A protein- an alternative way of insect control in cotton
Production and characterization of monoclonal antibody to bovine leukemia virus gp51-SU
Enzootic bovine leukemia (EBL) is a disease of cattle with worldwide distribution. The bovine leukemia virus (BLV), is a typical type C retrovirus, has four genes: gag, pol, pro and env, flanked by long terminal repeats (LTR) and lacking oncogenes. Gene env encodes a precursor protein named Pr72 env which undergoes glycosylation and lysis, giving rise to an envelope glycoprotein gp51-SU with a total of 12 epitopes. The objective of the present study was to produce a panel of monoclonal antibodies (mAbs) against BLV-gp51-SU following the standard immunization protocols. The antigen preparations included cellular lysates, a fraction of cellular lysates to study antibodies against BLV proteins precursors, whole virus particles and semipurified gp51 -SU. Both route of injection (SC and IP) resulted in hyperimmunized mice. As indicated by indirect-ELISA and also yielded high fusion efficiency. The hybridoma colonies were stable and had a tendency to save their ability to secrete antibodies. Fusions produced five positive colonies in screening tests, two of which were against gp51-SU. The targeted hydrophobic domain of the gp51-SU molecule was detected by one of the colonies of mAbs. The two interested mAbs were used for immunohistochemistry on lymph node sections of a cow with lymphosarcoma which can detect immunoreactive materials in sections and also in H&E staining. We conclude the produced panel of mAbs is able to be used in detection of gp51-SU and a new epitope identified on this molecule could be used in IHC method for diagnosis of BLV infection. © IDOSI Publications, 2011.Raziallah Jafari Joozani, Parvaneh Khazrai Nia, Mohammad Taheri, Farhid Hemmatzadeh and Javad Ashrafihela
A new economical scheme of acceptance sampling plan in a two-stage approach based on the maxima nomination sampling technique
Prediction of k-records from a general class of distributions under balanced type loss functions
Absolute value error loss, Balanced loss function, Bayes prediction, Conditional median prediction, Maximum likelihood prediction, LINEX loss, Record values,
Pure-mode correlation functions for cosmic shear and application to KiDS-1000
Large scale structure and cosmolog
Bayes sequential estimation for a particular exponential family of distributions under LINEX loss
AO rule, APO rule, Bayes sequential estimation, LINEX loss function, Transformed chi-square family of distributions, 62L12, 62F10, 62F15,