An assessment of nitrogen fixation in 'organically managed' spring-sown lupins and leaching under a following winter cereal

Three spring-sown species of lupins (Bora, Prima and Wodjil) and peas were compared in terms of N fixation and subsequent leaching under a following winter cereal crop. Although peas out-yielded lupins (5.4 t compared with ca 3.5 t grain, respectively), the yellow lupin (Wodjil) fixed more N than peas (180 compared with 120 kg N ha-1) and all three lupins contained more protein (> 30%) than the peas (22%). Wodjil was the most effective at suppressing weeds, carrying only 12% of the weed burden found in fallow plots, followed by peas (19%). Winter leaching amounted to > 50 kg nitrate-N ha-1 from under the winter cereal, regardless of whether the previous treatment was a legume crop or was left fallow. There were no significant differences in leaching between the three species of lupin. Leachate in the first 350 mm of drainage under the winter cereal exceeded the EU limit on nitrate in drinking water in all treatments. This work is part of a wider collaborative study supported by Defra which covers a range of UK sites

Set in Stone: Building America's New Generation of Arts Facilities, 1994-2008

In 2007, just before the domestic economy experienced a major trauma, the Cultural Policy Center at the Harris School and NORC at the University of Chicago launched a national study of cultural building in the United States. It was motivated by multiple requests from leading consultants in the cultural sector who found themselves involved in a steadily growing number of major building projects -- museums, performing arts centers (PACs), and theaters -- and from foundation officers who were frequently asked to help fund these infrastructure projects. With the generous support of the Andrew W. Mellon Foundation, the Kresge Foundation, and the John D. and Catherine T. MacArthur Foundation, we were able to conduct systematic scientific research on cultural building in the United States between 1994 and 2008 and come to a number of conclusions that have important implications for the cultural sector

Comparing Extraction Methods for Biomarker Steroid Characterisation from Soil and Slurry

Clean water is a precious resource, and policies/programmes are implemented worldwide to protect and/or improve water quality. Faecal pollution can be a key contributor to water quality decline causing eutrophication through nutrient enrichment and pathogenic contamination. The robust sourcing of faecal pollutants is important to be able to target the appropriate sector and to engage managers. Biomarker technology has the potential for source confirmation, by using, for example the biomarker suite of steroids. Steroids have been used in the differentiation of human and animal faeces; however, there is no unequivocal extraction technique. Some of the methods used include (i) Soxhlet extraction, (ii) Bligh and Dyer (BD) extraction, and (iii) accelerated solvent extraction (ASE). The less costly and time intensive technique of ASE is particularly attractive, but a current research gap concerns further comparisons regarding ASE lipid extraction from soils/slurries compared with the more traditional Soxhlet and BD extractions. Accordingly, a randomised complete block experiment was implemented to assess differences between the three extraction methods, differences between the different sample types, and the interactions between these two factors. Following GC-MS, it was found that there was no significant difference between the results of the steroid extraction methods, regardless of the type of sample used, for the quantity of each steroid extracted. It was concluded that ASE could be used confidently instead of the more established steroid extraction methods, thereby delivering time and cost savings

Field Experiments to Help Optimise Nitrogen Fixation by Legumes on Organic Farms

During an organic rotation, the aim is to increase the nitrogen (N) content of the soil (and hence build up soil fertility) by recycling crop residues, applying manures/composts and from N fixed by legumes. IGER, with ADAS, Duchy College Cornwall and Abacus Organic Associates are developing improved guidance for organic farmers in the use of fertility-building crops. The main questions are: how to maximise N fixation and how to make the most efficient use of the fixed N? Available soil mineral N, which is generally thought to reduce N fixation, will be increased by manure applications, cutting/mulching and grazing. We describe an experiment to establish the extent to which animal and green manures can adversely affect N fixation. The results from the first year (2003) were reported recently (Hatch et al., 2004). Here we summarise the findings from 2 years’ results (2003-4) to show the changes that occurred after the legume crop became fully established

Species and Genotype Effects of Bioenergy Crops on Root Production, Carbon and Nitrogen in Temperate Agricultural Soil

Bioenergy crops have a secondary benefit if they increase soil organic C (SOC) stocks through capture and allocation below-ground. The effects of four genotypes of short-rotation coppice willow (Salix spp., ‘Terra Nova’ and ‘Tora’) and Miscanthus (M. × giganteus (‘Giganteus’) and M. sinensis (‘Sinensis’)) on roots, SOC and total nitrogen (TN) were quantified to test whether below-ground biomass controls SOC and TN dynamics. Soil cores were collected under (‘plant’) and between plants (‘gap’) in a field experiment on a temperate agricultural silty clay loam after 4- and 6-years’ management. Root density was greater under Miscanthus for plant (up to 15.5 kg m–3) compared with gap (up to 2.7 kg m–3) whereas willow had lower densities (up to 3.7 kg m–3). Over two years, SOC increased below 0.2 m depth from 7.1 to 8.5 kg m–3 and was greatest under Sinensis at 0-0.1 m depth (24.8 kg m–3). Miscanthus-derived SOC, based on stable isotope analysis, was greater under plant (11.6 kg m–3) than gap (3.1 kg m–3) for Sinensis. Estimated SOC stock change rates over the two-year period to 1-m depth were 6.4 for Terra Nova, 7.4 for Tora, 3.1 for Giganteus and 8.8 Mg ha–1 year–1 for Sinensis. Rates of change of TN were much less. That SOC matched root mass down the profile, particularly under Miscanthus, indicated that perennial root systems are an important contributor. Willow and Miscanthus offer both biomass production and C sequestration when planted in arable soil

Facile Preparation of Organic Nanoparticles by Interfacial Cross-Linking of Reverse Micelles and Template Synthesis of Subnanometer Au−Pt Nanoparticles

A single- and a double-tailed cationic surfactant with the triallylammonium headgroup formed reverse micelles (RMs) in heptane/chloroform containing a small amount of water. The reverse micelles were cross-linked at the interface upon UV irradiation in the presence of a water-soluble dithiol cross-linker and a photoinitiator. The resulting interfacially cross-linked reverse micelles (ICRMs) of the single-tailed surfactant aggregated in a solvent-dependent fashion, whereas those of the double-tailed were identical in size as the corresponding RMs. The ICRMs could extract anionic metal salts, such as AuCl4− and PtCl62−, from water into the organic phase. Au and Pt metal nanoparticles were produced upon reduction of metal salts. The covalent nature of the ICRMs made the template synthesis highly predictable, with the size of the metal particles controlled by the amount of the metal salt and the method of reduction. Nanoalloys were obtained by combining two metal precursors in the same reaction. Reduction of the ICRM-entrapped aurate also occurred without any external reducing agents, and the gold nanoparticles differed dramatically from those obtained through sodium borohydride reduction. The same template allowed the preparation of luminescent Au4, Au8, and Au13−Au23 clusters, as well as gold nanoparticles several nanometers in size, simply by using different amounts of gold precursor and reducing conditions

Syndromics: A Bioinformatics Approach for Neurotrauma Research

Substantial scientific progress has been made in the past 50 years in delineating many of the biological mechanisms involved in the primary and secondary injuries following trauma to the spinal cord and brain. These advances have highlighted numerous potential therapeutic approaches that may help restore function after injury. Despite these advances, bench-to-bedside translation has remained elusive. Translational testing of novel therapies requires standardized measures of function for comparison across different laboratories, paradigms, and species. Although numerous functional assessments have been developed in animal models, it remains unclear how to best integrate this information to describe the complete translational “syndrome” produced by neurotrauma. The present paper describes a multivariate statistical framework for integrating diverse neurotrauma data and reviews the few papers to date that have taken an information-intensive approach for basic neurotrauma research. We argue that these papers can be described as the seminal works of a new field that we call “syndromics”, which aim to apply informatics tools to disease models to characterize the full set of mechanistic inter-relationships from multi-scale data. In the future, centralized databases of raw neurotrauma data will enable better syndromic approaches and aid future translational research, leading to more efficient testing regimens and more clinically relevant findings