52 research outputs found
A rapid and robust tri-color flow cytometry assay for monitoring malaria parasite development
Microscopic examination of Giemsa-stained thin blood smears remains the gold standard method used to quantify and stage malaria parasites. However, this technique is tedious, and requires trained microscopists. We have developed a fast and simple flow cytometry method to quantify and stage, various malaria parasites in red blood cells in whole blood or in vitro cultured Plasmodium falciparum. The parasites were stained with dihydroethidium and Hoechst 33342 or SYBR Green I and leukocytes were identified with an antibody against CD45. Depending on the DNA stains used, samples were analyzed using different models of flow cytometers. This protocol, which does not require any washing steps, allows infected red blood cells to be distinguished from leukocytes, as well as allowing non-infected reticulocytes and normocytes to be identified. It also allows assessing the proportion of parasites at different developmental stages. Lastly, we demonstrate how this technique can be applied to antimalarial drug testing
Spatio-temporal graph convolutional neural network for remaining useful life estimation of aircraft engines
Energy dependence of cross sections for double-electron capture in 48–132-keV C6+ + He collisions
International audienc
Proton exchange membrane fuel cell model for aging predictions: Simulated equivalent active surface area loss and comparisons with durability tests
SYNTHESIS OF ACYLATED ACTIVE METHYLENE COMPOUNDS WITH N-Boc-L-PHENYLALANINE AND THEIR HETEROCYCLIZATION, BOTH ACHIEVED ENANTIOSELECTIVELY
Convolutional neural network based on attention mechanism and Bi-LSTM for bearing remaining life prediction
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