Bulk Genotype Table

This file contains the allele counts (number of occurrences of an allele in a sequencing read) for each heterozygous site found in YJM311 from the YJM311 (parental), simple, intermediate, and complex se- quencing runs, and the G-statistic values at each of these sites for the pairwise comparisons between the simple, intermediate, and complex pools. Note that heterozygous sites where neither allele matched the reference sequence are very rare, so were excluded from these results. Each row in the file contains data on a single heterozygous site identified in YJM311. The coordinates are based on Saccharomyces Genome Database (SGD) release r62, (release date: February 18, 2009)

Mean eye errors performed by MFL, CF, and the control group.

<p>(A) Eye direction errors (B) hypometric steps (C) look-backs (D) hypermetric steps that have been normalized as a ratio per trial across all targets for four conditions (V = Vertical; VR = Vertical Rotated; H = Horizontal; HR = Horizontal Rotated). Both hands were pooled for MFL and CF. Note an increase in oculomotor errors for both MFL and CF during the conditions with rotated visual feedback (VR/HR). Error bars denote 95% Confidence Intervals. + No statistical comparison between the case and the control group could be performed because the control group had a mean and variance of zero. **<i>p</i>′<0.01; ***<i>p</i>′<0.001; ****<i>p</i>′<0.0001.</p

Ballistic hand endpoint data for MFL, CF, and the control group.

<p>Hand movement (A) on-axis constant error (B) off-axis constant error (C) variable error (in mm) for four conditions (V = Vertical; VR = Vertical Rotated; H = Horizontal; HR = Horizontal Rotated) across all targets. Both hands were pooled for MFL and CF. Error bars denote 95% Confidence Intervals. ***<i>p</i>′<0.001.</p

Eye movement error differences between MFL and CF compared with the control group.

<p><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0046619#pone-0046619-t002" target="_blank">Table 2</a> note: Dependent variables (DE = initial direction error; Look-backs = look-backs to hand or cursor; Hypo-steps = hypometric saccadic steps; Hyper-steps = hypermetric saccadic steps) were tested with separated modified t-tests (<i>p</i>′<0.05) for each condition (V = vertical; VR = vertical rotated; H = horizontal; HR = horizontal rotated) for each hand and each visual target (R = right; T = top; L = left; B = bottom). + No statistical comparison between the case and the control group could be performed because the control group had a mean and variance of zero.</p>*<p><i>p</i>′<0.01;</p>**<p><i>p</i>′<0.001;</p>***<p><i>p</i>′<0.0001.</p

Individual hand endpoint ellipses for MFL, CF, and a typical control subject.

<p>Hand movement endpoints to four peripheral targets from the home target in (A) Vertical (B) Vertical Rotated (C) Horizontal (D) Horizontal Rotated. Both hands were pooled for MFL and CF. Open and filled ellipses represent 95% confidence intervals for patients and a typical control, respectively. Circles with cross-hatching represent starting and ending target location. Note that the systematic undershoot seen in both patients is not seen in the horizontal conditions in the + y direction towards the monitor.</p

Task procedure, example patient eye data.

<p>(A) Schematic drawings of the standard center-out reaching movement towards one of four peripheral targets. Reaching movements were done both directly (vertical) and in two basic manipulations: spatial plane dissociation (horizontal) and 180° visuomotor rotation (vertical rotated) employed both separately and in combination (horizontal rotated), as well as a single arbitrary association task. In the arbitrary condition, the maple leaf symbol is shown to indicate a required upward hand and eye movement. (B) Example x (gray dots) and y (black dots) eye position (in mm) for OA patients towards right (positive x) peripheral target during the VR and H conditions. Gray lines represent x hand position and black line represents y hand position (positive is upward) from movement onset to movement offset (short black lines). Horizontal dashed lines demark the location of peripheral targets, while long vertical black lines represent the go signal. Note that the look-back in the H condition was to the cursor representation of the hand, not the hand itself, while the look-back in the VR condition was to the hand.</p

Hand movement timing data for MFL, CF, and the control group.

<p>Mean reaction times (A) ballistic movement times (B) and corrective movement times (C) in msec for both groups for the five conditions (V = Vertical; VR = Vertical Rotated; H = Horizontal; HR = Horizontal Rotated; ARB = Arbitrary) across all targets. Both hands were pooled for MFL and CF. Error bars denote 95% Confidence Intervals. *<i>p</i>′<0.05 ; **<i>p</i>′<0.01; ***<i>p</i>′<0.001; ****<i>p</i>′<0.0001.</p

Hand movement endpoint and error differences between MFL and CF compared with the control group.

<p><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0046619#pone-0046619-t002" target="_blank">Table 2</a> note: Dependent variables (CE = constant error; VE = variable error; DR 180° =  direction reversals in the opposite direction) were tested with separated modified t-tests (<i>p</i>′<0.05) for each condition (V = vertical; VR = vertical rotated; H = horizontal; HR = horizontal rotated) for each hand and each visual target (R = right; T = top; L = left; B = bottom).</p>*<p><i>p</i>′<0.01;</p>**<p><i>p</i>′<0.001.</p

Index of difficulty for the decoupled non-standard conditions relative to the standard condition.

<p>A positive number from 0–1 indicates that the decoupled conditions were more difficult than the standard conditions across all significant dependent variables. Note the marked increase in ID for both patients in the Vertical Rotated (VR) condition relative to the controls. Error bars denote 95% Confidence Intervals. *<i>p</i>′<0.05 ; **<i>p</i>′<0.01.</p

Anatomical MRI scan slices of patient CF (first row) and patient MFL (second row).

<p>The z-coordinates of the axial slices are indicated in blue. Occipital and parietal lesions were mapped and colored in green and red respectively. The major sulci are indicated to guide the localization of the lesions (Cal: calcarine, PO: parieto-occipital, IPS: intra-parietal, CS: central). Note that these MRI scans were acquired at the acute stage of the strokes and that at the time of testing no visual field defect was associated with the occipital lesions. The patients' lesions overlap to the greatest extent at the level of the right caudal superior parietal lobule, which is the pertinent anatomical substrate of their common chronic visuomotor deficits.</p