959 research outputs found
Magnetic Circular Dichroism of Cobalt-Copper and Zinc-Copper Bovine Superoxide Dismutase
Abstract The magnetic circular dichroism of the d-d transitions of Co(II) when substituted for Zn(II) in the Zn(II)-Cu(II) enzyme bovine superoxide dismutase enzyme is reported. Magnetic circular dichroism of the Co(II) chromophore in the Co(II)-Cu(II) enzyme is typical of tetrahedral Co(II) compounds. The magnetic circular dichroism band pattern is almost identical with the magnetic circular dichroism of the anion complexes of Co(II) carbonic anhydrase, implying similar coordination geometries in the two enzyme Co(II) complexes. The Cu(II) chromophore is only weakly induced by the magnetic field, with induced ellipticity an order of magnitude less than that of the Co(II) chromophore. Reduction of the Co(II)-Cu(II) protein causes minor, but significant, changes in the Co(II) site as measured by magnetic circular dichroism
Exploring \pp scattering in the \1N picture
In the large approximation to , the leading \pp scattering
amplitude is expressed as the sum of an infinite number of tree diagrams. We
investigate the possibility that an adequate approximation at energies up to
somewhat more than one can be made by keeping diagrams which involve the
exchange of resonances in this energy range in addition to the simplest chiral
contact terms. In this approach crossing symmetry is automatic but individual
terms tend to drastically violate partial wave unitarity. We first note that
the introduction of the meson in a chirally invariant manner
substantially delays the onset of drastic unitarity violation which would be
present for the {\it current algebra} term alone. This suggests a possibility
of local (in energy) cancellation which we then explore in a phenomenological
way. We include exchanges of leading resonances up to the region.
However, unitarity requires more structure which we model by a four derivative
contact term or by a low lying scalar resonance which is presumably subleading
in the \1N expansion, but may nevertheless be important. The latter two
flavor model gives a reasonable description of the phase shift up
until around , before the effects associated which the
threshold come into play.Comment: 27 LaTex pages + 13 figures (also available in hard-copy
Quality of Private Ground-Water Supplies in Kentucky
About 3.7 million people live in Kentucky, of which 1.9 million (52 percent) live in urban areas (roughly defined as any community with 2,500 or more people) and 1.8 million (48 percent) live in rural areas (University of Kentucky, 1993). Figure 1 summarizes sources of drinking water for Kentucky residents. About 70 percent of Kentuckians get their daily supply of water from surface-water sources - lakes and streams; about 25 percent get their water from ground-water wells; and about 5 percent get their water from other sources - springs, cisterns, ponds, or hauled water
Phase 2 Study of Pemetrexed Plus Carboplatin, or Pemetrexed Plus Cisplatin with Concurrent Radiation Therapy Followed by Pemetrexed Consolidation in Patients with Favorable-Prognosis Inoperable Stage IIIA/B NonāSmall-Cell Lung Cancer
IntroductionThere is no consensus chemotherapy regimen with concurrent radiotherapy (RT) for inoperable stage IIIA/B nonāsmall-cell lung cancer. This trial evaluated pemetrexed with carboplatin (PCb) or cisplatin (PC) with concurrent RT followed by consolidation pemetrexed.MethodsIn this open-label, noncomparative phase II trial, patients with inoperable stage IIIA/B nonāsmall-cell lung cancer (initially all histologies, later restricted to nonsquamous) were randomized (1:1) to PCb or PC with concurrent RT (64ā68 Gy over days 1ā45). Consolidation pemetrexed monotherapy was administered every 21 days for three cycles. Primary endpoint was 2-year overall survival (OS) rate.ResultsFrom June 2007 to November 2009, 98 patients were enrolled (PCb: 46; PC: 52). The 2-year OS rate was PCb: 45.4% (95% confidence interval [CI], 29.5ā60.0%); PC: 58.4% (95% CI, 42.6ā71.3%), and in nonsquamous patients was PCb: 48.0% (95% CI, 29.0ā64.8%); PC: 55.8% (95% CI, 38.0ā70.3%). Median time to disease progression was PCb: 8.8 months (95% CI, 6.0ā12.6 months); PC: 13.1 months (95% CI, 8.3ānot evaluable [NE]). Median OS (months) was PCb: 18.7 (95% CI, 12.9āNE); PC: 27.0 (95% CI, 23.2āNE). The objective response rates (ORRs) were PCb: 52.2%; PC: 46.2%. Grade 4 treatment-related toxicities (% PCb/% PC) were: anemia, 0/1.9; neutropenia, 6.5/3.8; thrombocytopenia, 4.3/1.9; and esophagitis, 0/1.9. Most patients completed scheduled chemotherapy and RT during induction and consolidation phases. No drug-related deaths were reported during chemoradiotherapy.ConclusionsBecause of study design, efficacy comparisons cannot be made. However, both combinations with concurrent RT were active and well tolerated
Portal protein diversity and phage ecology
Ā© 2008 The Authors. This article is distributed under the terms of the Creative Commons License, Attribution 2.5. The definitive version was published in Environmental Microbiology 10 (2008): 2810-2823, doi:10.1111/j.1462-2920.2008.01702.x.Oceanic phages are critical components of the global ecosystem, where they play a role in microbial mortality and evolution. Our understanding of phage diversity is greatly limited by the lack of useful genetic diversity measures. Previous studies, focusing on myophages that infect the marine cyanobacterium Synechococcus, have used the coliphage T4 portal-protein-encoding homologue, gene 20 (g20), as a diversity marker. These studies revealed 10 sequence clusters, 9 oceanic and 1 freshwater, where only 3 contained cultured representatives. We sequenced g20 from 38 marine myophages isolated using a diversity of Synechococcus and Prochlorococcus hosts to see if any would fall into the clusters that lacked cultured representatives. On the contrary, all fell into the three clusters that already contained sequences from cultured phages. Further, there was no obvious relationship between host of isolation, or host range, and g20 sequence similarity. We next expanded our analyses to all available g20 sequences (769 sequences), which include PCR amplicons from wild uncultured phages, non-PCR amplified sequences identified in the Global Ocean Survey (GOS) metagenomic database, as well as sequences from cultured phages, to evaluate the relationship between g20 sequence clusters and habitat features from which the phage sequences were isolated. Even in this meta-data set, very few sequences fell into the sequence clusters without cultured representatives, suggesting that the latter are very rare, or sequencing artefacts. In contrast, sequences most similar to the culture-containing clusters, the freshwater cluster and two novel clusters, were more highly represented, with one particular culture-containing cluster representing the dominant g20 genotype in the unamplified GOS sequence data. Finally, while some g20 sequences were non-randomly distributed with respect to habitat, there were always numerous exceptions to general patterns, indicating that phage portal proteins are not good predictors of a phage's host or the habitat in which a particular phage may thrive.This research was supported in part by funding from NSF (CMORE contribution #87), DOE, The Seaver Foundation and the Gordon and Betty Moore Foundation Marine Microbiology Program to S.W.C.; an NIH Bioinformatics Training Grant supported M.B.S.; MIT Undergraduate Research Opportunities Program supported V.Q., J.A.L., G.T., R.F. and J.E.R.; Howard Hughes Medical Institute funded MIT Biology Department Undergraduate Research Opportunities Program supported A.S.D.; NSERC (Canada) Discovery Grant (DG 298394) and a Grant from the Canadian Foundation for Innovation (NOF10394) to J.P.B.; NSF Graduate Fellowship funding supported M.L.C
Corrigendum "Portal protein diversity and phage ecology"
Author Posting. Ā© The Author(s), 2011. This is the author's version of the work. It is posted here by permission of John Wiley & Sons for personal use, not for redistribution. The definitive version was published in Environmental Microbiology 13 (2011): 2832, doi:10.1111/j.1462-2920.2011.02616.x
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