Low-cost Soil Moisture Sensors Based on Inductive Coils Tested on Different Sorts of Soils

[EN] The use of precision agriculture and the Internet of Things has improved the efficiency of many cultures. Nevertheless, there are a few low-cost options to monitor soil moisture. Moreover, those options depend on the specific characteristics of the soil. In this paper, we attempt to find a sensor, based on mutual inductance, that could be used for more than one sort of soil. We study three prototypes, one of them with casing. The sensors are powered with a voltage of 10 peak to peak volts. One of the soils has a high content of organic matter and sand while the other is rich in sand and silt. The best prototype for the soil with high levels of organic matter has 10 turns on the powered coil and 5 on the induced coil. The best frequency for this sensor is 1340 kHz. For the soil with a significant quantity of silt, the best prototype has 80 turns on the powered coil and 40 on the induced coil. The frequency at which this sensor works best is 229 kHz, which happens to be its peak frequency. With those characteristics regressions lines with R2 values higher than 0.75 can be modeledThis work is partially found by the Conselleria de Educación, Cultura y Deporte with the Subvenciones para la contratación de personal investigador en fase postdoctoral, grant number APOSTD/2019/04, by European Union through the ERANETMED (Euromediterranean Cooperation through ERANET joint activities and beyond) project ERANETMED3-227 SMARTWATIR, and by the European Union with the Fondo Europeo Agrícola de Desarrollo Rural (FEADER) Europa invierte en zonas rurales, the MAPAMA, and Comunidad de Madrid with the IMIDRA, under the mark of the PDR-CM 2014-2020 project number PDR18-XEROCESPED.Parra-Boronat, M.; Parra-Boronat, L.; Lloret, J.; Mauri, PV.; Llinares Palacios, JV. (2019). Low-cost Soil Moisture Sensors Based on Inductive Coils Tested on Different Sorts of Soils. IEEE. 616-622. https://doi.org/10.1109/IOTSMS48152.2019.8939258S61662

Environmentally induced changes in antioxidant phenolic compounds levels in wild plants

[EN] Different adverse environmental conditions cause oxidative stress in plants by generation of reactive oxygen species (ROS). Accordingly, a general response to abiotic stress is the activation of enzymatic and non-enzymatic antioxidant systems. Many phenolic compounds, especially flavonoids, are known antioxidants and efficient ROS scavengers in vitro, but their exact role in plant stress responses in nature is still under debate. The aim of our work is to investigate this role by correlating the degree of environmental stress with phenolic and flavonoid levels in stress-tolerant plants. Total phenolic and antioxidant flavonoid contents were determined in 19 wild species. Meteorological data and plant and soil samples were collected in three successive seasons from four Mediterranean ecosystems: salt marsh, dune, semiarid and gypsum habitats. Changes in phenolic and flavonoid levels were correlated with the environmental conditions of the plants and were found to depend on both the taxonomy and ecology of the investigated species. Despite species-specific differences, principal component analyses of the results established a positive correlation between plant phenolics and several environmental parameters, such as altitude, and those related to water stress: temperature, evapotranspiration, and soil water deficit. The correlation with salt stress was, however, very weak. The joint analysis of all the species showed the lowest phenolic and flavonoid levels in the halophytes from the salt marsh. This finding supports previous data indicating that the halophytes analysed here do not undergo oxidative stress in their natural habitat and therefore do not need to activate antioxidant systems as a defence against salinity.This work has been funded by the Spanish Ministry of Science and Innovation (Project CGL2008-00438/BOS), with contribution from the European Regional Development Fund. Thanks to Dr. Rafael Herrera for critical reading of the manuscript.Bautista, I.; Boscaiu, M.; Lidón, A.; Llinares Palacios, JV.; Lull, C.; Donat-Torres, MP.; Mayoral García-Berlanga, O.... (2016). Environmentally induced changes in antioxidant phenolic compounds levels in wild plants. Acta Physiologiae Plantarum. 38(1):1-15. https://doi.org/10.1007/s11738-015-2025-2S115381Agati G, Biricolti S, Guidi L, Ferrini F, Fini A, Tattini M (2011) The biosynthesis of flavonoids is enhanced similarly by UV radiation and root zone salinity in L. vulgare leaves. 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Is salinity the main ecologic factor that shapes the distribution of two endemic Mediterranean plant species of the genus Gypsophila?

The final publication is available at Springer via http://dx.doi.org/10.1007/s11104-014-2218-2Aims Responses to salt stress of two Gypsophila species that share territory, but with different ecological optima and distribution ranges, were analysed. G. struthium is a regionally dominant Iberian endemic gypsophyte, whereas G. tomentosa is a narrow endemic reported as halophyte. Theworking hypothesis is that salt tolerance shapes the presence of these species in their specific habitats. Methods Taking a multidisciplinary approach, we assessed the soil characteristics and vegetation structure at the sampling site, seed germination and seedling development, growth and flowering, synthesis of proline and cation accumulation under artificial conditions of increasing salt stress and effect of PEG on germination and seedling development. Results Soil salinity was low at the all sampling points where the two species grow, but moisture was higher in the area of G. tomentosa. Differences were found in the species salt and drought tolerance. The different parameters tested did not show a clear pattern indicating the main role of salt tolerance in plant distribution. Conclusions G. tomentosa cannot be considered a true halophyte as previously reported because it is unable to complete its life cycle under salinity. The presence of G. tomentosa in habitats bordering salt marshes is a strategy to avoid plant competition and extreme water stressSoriano, P.; Moruno Manchón, JF.; Boscaiu Neagu, MT.; Vicente Meana, Ó.; Hurtado, A.; Llinares Palacios, JV.; Estrelles, E. (2014). Is salinity the main ecologic factor that shapes the distribution of two endemic Mediterranean plant species of the genus Gypsophila?. Plant and Soil. 384(1-2):363-379. doi:10.1007/s11104-014-2218-2S3633793841-2Alonso MA (1996) Flora y vegetación del Valle de Villena (Alicante). 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Effects of Salt Stress on Three Ecologically Distinct Plantago Species

Comparative studies on the responses to salt stress of taxonomically related taxa should help to elucidate relevant mechanisms of stress tolerance in plants. We have applied this strategy to three Plantago species adapted to different natural habitats, P. crassifolia and P. coronopus both halophytes and P. major, considered as salt-sensitive since it is never found in natural saline habitats. Growth inhibition measurements in controlled salt treatments indicated, however, that P. major is quite resistant to salt stress, although less than its halophytic congeners. The contents of monovalent ions and specific osmolytes were determined in plant leaves after four-week salt treatments. Salt-treated plants of the three taxa accumulated Na+ and Cl- in response to increasing external NaCl concentrations, to a lesser extent in P. major than in the halophytes; the latter species also showed higher ion contents in the non-stressed plants. In the halophytes, K+ concentration decreased at moderate salinity levels, to increase again under high salt conditions, whereas in P. major K+ contents were reduced only above 400 mM NaCl. Sorbitol contents augmented in all plants, roughly in parallel with increasing salinity, but the relative increments and the absolute values reached did not differ much in the three taxa. On the contrary, a strong (relative) accumulation of proline in response to high salt concentrations (600 800 mM NaCl) was observed in the halophytes, but not in P. major. These results indicate that the responses to salt stress triggered specifically in the halophytes, and therefore the most relevant for tolerance in the genus Plantago are: a higher efficiency in the transport of toxic ions to the leaves, the capacity to use inorganic ions as osmotica, even under low salinity conditions, and the activation, in response to very high salt concentrations, of proline accumulation and K+ transport to the leaves of the plants.MAH was a recipient of an Erasmus Mundus pre-doctoral scholarship financed by the European Commission (Welcome Consortium). AP acknowledges the Erasmus mobility programme for funding her stay in Valencia to carry out her Master Thesis.Al Hassan, M.; Pacurar, AM.; López Gresa, MP.; Donat Torres, MDP.; Llinares Palacios, JV.; Boscaiu Neagu, MT.; Vicente Meana, Ó. (2016). Effects of Salt Stress on Three Ecologically Distinct Plantago Species. PLoS ONE. 11(8):1-21. doi:10.1371/journal.pone.0160236S12111

Comparative Analysis of Tolerance to Salt Stress and Water Deficit in Two Invasive Weeds of the Genus Erigeron (Asteraceae)

Erigeron bonariensis and E. sumatrensis are two noxious weeds present in many parts of the world. Their tolerance to salinity and water deficit was analysed at the seed germination stage and during vegetative development. Seed germination was tested in solutions with different concentrations of NaCl and polyethylene glycol (PEG). Growth parameters, photosynthetic pigments, ion accumulation, and antioxidant mechanisms were analysed in plants that were subjected to increasing NaCl solutions, or severe water deficit by completely restricting irrigation. Seed germination was mostly affected by NaCl, but less by PEG in both species. E. bonariensis had a faster germination in all treatments and maintained a higher percentage of germination under the highest concentration of salt applied. Growth responses were similar in the two species, both being more affected by higher salt concentrations than by water deficit. The main differences in the responses of the two species to stress regard K+ and proline concentration. K+ in roots decreased under salt stress in E. sumatrensis, but remained constant in leaves, whereas in E. bonariensis increased in roots and leaves in salt-stressed plants. Proline concentration increased in all E. bonariensis plants under salt stress, but only in those under the highest salt concentration in E. sumatrensis. The results obtained indicate that the two species are relatively tolerant to water deficit and medium salinity but are susceptible to high NaCl concentrations

Principal Component Analyses (PCAs).

<p>Changes in all measured growth parameters and biochemical stress markers were analyzed in salt-treated plants with respect to the control, non-stressed plants of <i>Plantago crassifolia</i> (A), <i>P</i>. <i>coronopus</i> (B) and <i>P</i>. <i>major</i> (C), in correlation to EC (electrical conductivity) of the substrate, at the end of the four-week treatment. Abbreviations: number of leaves (NL), fresh weight percentage (FW%), water content percentage (WC%), sodium ions (Na⁺), potassium ions (K⁺), chloride ions (Cl^-), sorbitol (Sor), proline (Pro), glycine-betaine (GB), total soluble sugars (TSS).</p

Salt-induced inhibition of plant growth.

<p>Number of leaves of <i>Plantago</i> plants after four weeks of treatement with the indicated NaCl concentrations (means ± SD, n = 5). Different lower case letters within each species indicate significant differences among treatments according to Tukey test (α = 0.05).</p

Salt-induced leaf dehydration.

<p>Leaf water content (%) after four weeks of treatment with increasing NaCl concentrations, in the three selected <i>Plantago</i> species (means ± SD, n = 5). Different lower case letters within each species indicate significant differences among treatments according to Tukey test (α = 0.05).</p

Inhibition of plant growth in the presence of increasing salt concentrations.

<p>Leaf fresh weight after four weeks of treatment with the indicated NaCl concentrations, for the three tested <i>Plantago</i> species. For each species, values (means ± SD, n = 5) are shown as percentages of the mean FW of the control plants, considered as 100% (absolute values: 11.35 ± 1.84 g, 10.61 ± 0.86 g, and 25.83 ± 3.51 g for <i>P</i>. <i>crassifolia</i>, <i>P</i>. <i>coronopus</i> and <i>P</i>. <i>major</i>, respectively). Different lower case letters within each species indicate significant differences among treatments according to Tukey test (α = 0.05).</p

Sorbitol accumulation upon salt treatments of <i>Plantago</i> plants.

<p>Sorbitol (Sor) leaf contents after four weeks of treatement with the indicated NaCl concentrations, in the three selected <i>Plantago</i> species (means ± SD, n = 5). Different lower case letters indicate significant differences among treatments according to Tukey test (α = 0.05).</p