Enhancement of antimicrobial activity of antibiotics and antifungals by the use of natural products from Pityrogramma calomelanos (L.) link

The ethanol extract and methanol fraction of Pityrogramma calomelanos (L.) link were evaluated for antibacterial, antifungal and modulatory activities against strains of Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Candida albicans, C. krusei and C. tropicalis. The antimicrobial activity of the natural products was evaluated by the microdilution method associated or not with aminoglycosides and antifungals. The ethanol extract and methanol fraction of P. calomelanos showed good activity against S. aureus when associated with aminoglycosides and with benzoilmetronidazol against species of the genus Candida. These results indicate that P. calomelanos should be studied as a possible source of natural products to combat bacteria and fungi either directly or by modulating the mechanisms of resistance of these microorganisms, enhancing the antimicrobial activity of these drugs and combating microbial resistance

Enhancement of antibiotic activity by Cordia verbenacea DC

Escherichia coli is known to produce enterotoxins whose properties and its role in diarrheal disease has been extensively investigated. Some species of Staphylococcus are often recognized as etiological agents of many animal and human opportunistic infections. This study is the first test of change in resistance of antibiotic activity by Cordia verbenacea DC. against multiresistant strains of Escherichia coli and Staphylococcus aureus. In this study, the hexane and methanol extract of Cordia verbenacea DC. were tested for antibacterial activity alone and in combination with aminoglycosides against bacterial strains. The synergy of the methanolic and hexane were verified by microdilution method. A synergistic effect of both extracts combined with the aminoglycosides was demonstrated. It is therefore suggested that the extracts from Cordia verbenacea DC. could be used as a source of natural products derived from this plant with resistance-modifying antibacterial activity, providing a new weapon against the problem of bacterial resistance to antibiotics.Colegio de Farmacéuticos de la Provincia de Buenos Aire

Enhancement of antibiotic activity by Cordia verbenacea DC

Escherichia coli is known to produce enterotoxins whose properties and its role in diarrheal disease has been extensively investigated. Some species of Staphylococcus are often recognized as etiological agents of many animal and human opportunistic infections. This study is the first test of change in resistance of antibiotic activity by Cordia verbenacea DC. against multiresistant strains of Escherichia coli and Staphylococcus aureus. In this study, the hexane and methanol extract of Cordia verbenacea DC. were tested for antibacterial activity alone and in combination with aminoglycosides against bacterial strains. The synergy of the methanolic and hexane were verified by microdilution method. A synergistic effect of both extracts combined with the aminoglycosides was demonstrated. It is therefore suggested that the extracts from Cordia verbenacea DC. could be used as a source of natural products derived from this plant with resistance-modifying antibacterial activity, providing a new weapon against the problem of bacterial resistance to antibiotics.Colegio de Farmacéuticos de la Provincia de Buenos Aire

Enhancement of antibiotic activity by Cordia verbenacea DC

Escherichia coli is known to produce enterotoxins whose properties and its role in diarrheal disease has been extensively investigated. Some species of Staphylococcus are often recognized as etiological agents of many animal and human opportunistic infections. This study is the first test of change in resistance of antibiotic activity by Cordia verbenacea DC. against multiresistant strains of Escherichia coli and Staphylococcus aureus. In this study, the hexane and methanol extract of Cordia verbenacea DC. were tested for antibacterial activity alone and in combination with aminoglycosides against bacterial strains. The synergy of the methanolic and hexane were verified by microdilution method. A synergistic effect of both extracts combined with the aminoglycosides was demonstrated. It is therefore suggested that the extracts from Cordia verbenacea DC. could be used as a source of natural products derived from this plant with resistance-modifying antibacterial activity, providing a new weapon against the problem of bacterial resistance to antibiotics.Colegio de Farmacéuticos de la Provincia de Buenos Aire

Chemical composition and toxicity of essential oils of Piper spp. against larvae of Aedes aegypti L. (Diptera: Culicidae)

Aedes aegypti L. é um dos vetores do dengue. No Brasil, tem ganhado muita atenção no setor da saúde pública, uma vez que esta doença tem se tornando mais agressiva na forma hemorrágica na popula- ção. Esse estudo teve como objetivo investigar o efeito de óleos essenciais de Piper aduncum, Piper marginatum e Piper nigrum contra larvas de Aedes aegypti. Em um esforço para encontrar uma maneira natural, eficaz e acessível para controlar esta doença endêmica, as atividades dos óleos essenciais, a partir das plantas, foram analisadas por comparação através da medida da CL50 . Os óleos essenciais obtidos por hidrodestilação foram analisados por CG/EM. Os principais componentes identificados foram: β-pineno (32,7%) e E-cariofileno (17,1%) em P. aduncum; isoelemecina (21,7%) e apiol (20,1%) em P. marginatum e E-cariofileno (24,2%) e Óxido-cariofileno (20,1%) em P. nigrum. Os resultados mostram que Piper marginatum apresentou CL50 de 8,29 μg/mL e este trabalho é o primeiro relato de atividade larvicida de P. aduncum. Estes resultados sugerem que os óleos essenciais de espécies do gênero Piper são promissores como larvicidas contra larvas de A. aegypti.Aedes aegypti L. is one of the vectors of dengue fever. In Brazil it has gain much attention in the Public Health sector since this disease has becoming more aggressive in the hemorrhagic form in the population. This study aimed to investigate the effect of Piper aduncum, Piper marginatum and Piper nigrum essential oils against Aedes aegypti larvae. In an effort to find a natural effective and affordable way to control this endemic disease, the larvicidal activities of essential oils from the plants were analyzed for activity comparison by measurement of their LC50 . The essential oils isolated by hydrodistillation were analyzed by GC/MS. The main components identified were: β-pinene (32.7%) and E-caryophyllene (17.1%) in P. aduncum; isoelemecin (21.7%) and apiole (20.1%) in P. marginatum and E-caryophyllene (24.2%) and caryophyllene oxide (20.1%) in P. nigrum. The results show that Piper marginatum presented the LC50 of 8.29 μg/mL and these are the first report about the larvicidal activity of P. aduncum. These results suggest that the essential oil of Piper species are promising as larvicide against A. aegypti larvae.Colegio de Farmacéuticos de la Provincia de Buenos Aire