H2O2 production in Col0

Production of ROS in Col-0. Confocal JPEG files and 8 bits ImageJ converted images. Col-0 leaves after exposure to aphid species Myzus persicae (Mp), M. cerasi (Mc) and Rhopalosihpum padi (Rp) and collected after 3, 6,12 and 24 hours. 2 replicates, R1 and R2

H2O2 production in NADPH-oxidase mutants

Production of ROS in Arabidopsis NADPH-oxidase mutants upon aphid interactions. Confocal JPEG files and 8 bits ImageJ converted images. Leaves of Col-0, the atrbohD-3 and atrbohF-3 mutant lines after exposure to aphids species Myzus persicae (Mp), M. cerasi (Mc) and Rhopalosihpum padi (Rp) and were collected after 3 and 24 hours. 3 replicates, R1, R2 and R3

H2O2 production in Col0 after aphid moult exposure

Production of ROS in Col-0. Confocal JPEG files and 8 bits ImageJ converted images. Col-0 leaves after exposure to aphid skin of Myzus persicae (Mp), M. cerasi (Mc) and Rhopalosihpum padi (Rp) and collected after 24 hours.

In Planta Expression Screens of Phytophthora infestans RXLR Effectors Reveal Diverse Phenotypes, Including Activation of the Solanum bulbocastanum Disease Resistance Protein Rpi-blb2[W]

The Irish potato famine pathogen Phytophthora infestans is predicted to secrete hundreds of effector proteins. To address the challenge of assigning biological functions to computationally predicted effector genes, we combined allele mining with high-throughput in planta expression. We developed a library of 62 infection-ready P. infestans RXLR effector clones, obtained using primer pairs corresponding to 32 genes and assigned activities to several of these genes. This approach revealed that 16 of the 62 examined effectors cause phenotypes when expressed inside plant cells. Besides the well-studied AVR3a effector, two additional effectors, PexRD8 and PexRD3645-1, suppressed the hypersensitive cell death triggered by the elicitin INF1, another secreted protein of P. infestans. One effector, PexRD2, promoted cell death in Nicotiana benthamiana and other solanaceous plants. Finally, two families of effectors induced hypersensitive cell death specifically in the presence of the Solanum bulbocastanum late blight resistance genes Rpi-blb1 and Rpi-blb2, thereby exhibiting the activities expected for Avrblb1 and Avrblb2. The AVRblb2 family was then studied in more detail and found to be highly variable and under diversifying selection in P. infestans. Structure-function experiments indicated that a 34–amino acid region in the C-terminal half of AVRblb2 is sufficient for triggering Rpi-blb2 hypersensitivity and that a single positively selected AVRblb2 residue is critical for recognition by Rpi-blb2